Cellular Compatibility and Antibacterial Properties of Magnesium Alloy after Ultrasonic Microarc Oxidation-doped Zn-HA Silane-phytic Acid Treatment.

doi:10.13701/j.cnki.kqyxyj.2019.10.021

Abstract

Abstract: Objective: To test the cellular biocompatibility and antibacterial property of magnesium alloy after ultrasonic microarc oxidation-doped Zn-HA silane-phytic acid treatment. Methods: The magnesium alloy UMAO-silane-phytic was designed as the control group. Zn-HA at concentration of 2 g/L, 4 g/L, and 6 g/L were added respectively as the experimental group. The compatibility of different treatment membrane cells was detected by CCK-8 kit, scanning electron microscope, and laser confocal microscope. Acridine orange/ethidium bromide (AO/EB) double fluorescent staining was used to detect the antibacterial. Results: The optical density (OD) value of cell adhesion and proliferation measured by CCK-8. The OD values were in the order at: group C>group D>group B>group A. The　number and shape of cells in the group C had the best extension. The antibacterial of 6 g/L Zn-HA group was obviously superior to other groups. Conclusion: The UMAO-doped 4 g/L Zn-HA silane-phytic acid treatment of magnesium alloy had the best biocompatibility. The UMAO-doped 6g/L Zn-HA silane-phytic acid treatment of magnesium alloy had the strongest antibacterial ability.

Key words: magnesium alloy, ultrasonic microarc oxidation, Zn-HA, silane phytic acid, cellular compatibility, antibacterial, properties

ZHANG Peng, ZHOU Libo, ZHANG Huiming, ZHANG Meiling, JIANG Feng, HUANG Yubo, LI Muqin, LI Dechao. Cellular Compatibility and Antibacterial Properties of Magnesium Alloy after Ultrasonic Microarc Oxidation-doped Zn-HA Silane-phytic Acid Treatment.[J]. Journal of Oral Science Research, 2019, 35(10): 1001-1004.

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