Abstract: Objective: To study the effects of concentrated growth factors on the expression of BSP, Runx2, and ALP, and to seek the mechanism of concentrated growth factors stimulating the osseointegration of titanium implants in rats. Methods: In vitro experiment, MTT and RT-qPCR were used to detect the proliferation and the expression of BSP, Runx2, and ALP in osteoblasts. In vivo experiment, the model of the osseointegration of titanium implants in the femora of rats was established. Micro-CT was used to analyze the bone mineral density, percent bone volume, and intersection surface. Results: Concentrated growth factors significantly increased the proliferation and the expression of BSP, Runx2, and ALP in osteoblasts. In vivo experiment, 8 weeks after implantation, concentrated growth factors significantly increased the bone mineral density, percent bone volume, and intersection surface. Conclusion: Concentrated growth factor may promote the osseointegration of titanium implants in rats by increasing the expression of osteoblast differentiation related genes, such as BSP, Runx2, and ALP.

Key words: Concentration growth factor, Osteoblast Implant