Effects of Tanshinol on RANKL/OPG of Alveolar Osteoblasts

doi:10.13701/j.cnki.kqyxyj.2019.09.018

Abstract

Abstract: Objective: To investigate the effects of tanshinol on the RANKL/OPG expression of alveolar osteoblasts. Methods: Alveolar osteoblasts of SD rats were cultured by primary cell culture. The alveolar osteoblasts of the fourth generation were treated with different concentrations (0.01, 0.10, 0.50, 1.00, 5.00 mg/L) of tanshinol serum. The proliferation rate of osteoblasts and the mRNA and protein expression levels of OGP and RANKL were detected by MTT, RT-PCR, and western blotting after the use of different concentrations of tanshinol serum on 1 d, 3 d, and 5 d, respectively. Results: The effect of tanshinol on the proliferation of alveolar osteoblasts varied with concentration and time of drugs. The proliferation rate was the highest when the alveolar osteoblasts were treated with 5 mg/L tanshinol at day 3. Compared with the control group, OGP mRNA and protein expression increased (P<0.05). Conclusion: Tanshinol could promote the proliferation of alveolar osteoblasts by increasing the expression of OGP mRNA and protein.

Key words: osteoblast, OPG/RANKL, alveolar bone, osteoporosis, tanshinol

ZHANG Xiaoyan, LIU Yunxin, WU Tie. Effects of Tanshinol on RANKL/OPG of Alveolar Osteoblasts[J]. Journal of Oral Science Research, 2019, 35(9): 894-897.

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