Journal of Oral Science Research

Expression of NLRP3/Caspase-1 Inflammation Pathway in Induced Rat Periapical Lesions.

WANG Jiao-jiao, WANG Li-na, YE Dan-dan, AO Xiang, DING Yin-hao, QU Wen-shu, NIU Wei-dong.

2016, 32(6): 549-553. DOI: 10.13701/j.cnki.kqyxyj.2016.06.001

Abstract ( 500 )

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Objective: To investigate the expression of NLRP3/Caspase-1 inflammation pathway in induced rat periapical lesions. Methods: 30 Sprague-Dawley (SD) rats were selected. The pulp cavities of the bilateral mandibular first molars were opened and sealed with phosphate buffer saline (PBS) and glass ionomer. The rats were randomly sacrificed at 0, 7, 14, 21 and 28 days. The mandibles were extracted forhematoxylin-eosin (HE) staining to observe the inflammation conditions in the apical area. The expression of NLRP3, Caspase-1 and IL-1β were examined by immunohistochemistry and real-time PCR. Results: NLRP3, Caspase-1 and IL-1β could be detected in the inflammatory periapical tissues, and the expression were remarkably increased when compared with the control group (P<0.05). The number of Caspase-1 and IL-1β positive cells were significantly correlated with the number of NLRP3 positive cells in the periapical lesions (P<0.01). Conclusion: The expression pattern of NLRP3/Caspase-1 inflammation pathway in experimental periapical lesions suggested that NLRP3-mediated signaling pathway might play an important role in periapical immune defense.

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Direct Co-culture of Synovial Stem Cells and Chondrocytes Induces Enhanced Cartilage Tissue Formation.

SHAO Bo, HU Lu-lu, GONG Zhong-cheng, YANG Meng, NING Xiao-ting, WANG Yue, HU Xin, LIU Hui, KEREMU·ABAShi, LIN Bin, YING Xiao-peng, GAO Yun-zu, LIN Zao-quan.

2016, 32(6): 554-558. DOI: 10.13701/j.cnki.kqyxyj.2016.06.002

Abstract ( 236 )

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Objective: To discuss direct co-culture of rat chondrocytes and synovial-derived mesenchymal stem cells at 1:1 and evaluate chondrocytes induced differentiation of stem cells in vitro. Methods: Rat synovial-derived mesenchymal stem cells and chondrocytes were cultivated in vitro in the same medium. The 3rd generation of synovial-derived mesenchymal stem cells and the 1st generation of chondrocytes in the proportion of 1:1 were mixed. The pellet at the final concentration of 1×10⁶/mL was set as the experimental group, and the considerable population of chondrocytes and synovial-derived mesenchymal stem cells was set as the control group. After cultivated in vitro for 21 days, the newly-born cartilage was evaluated through morphological observation, histological stain, and RT-PCR detection products. Results: After cultivated for 21 days, pellet chondroid tissue was formed and presented tenacious texture and milk white. Type-II collagen was identified by immunohistochemical staining. Matrix could be stained by Type-II collagen. RT-PCR results indicated that pellet expressed cartilage specific genes Type-II collagen and proteoglycan. Conclusion: Through direct co-culture, synovial-derived mesenchymal stem cells and chondrocytes can form mature cartilage.

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Cytocompatibility of Ultrasonic Micro-arc Oxidation-sodium Alginate-chitosan Composite Coating on Pure Titanium.

LI De-chao, HE Meng-qiao, LI Mu-qin, GUO Xiao-juan, MENG Xiang-cai.

2016, 32(6): 559-563. DOI: 10.13701/j.cnki.kqyxyj.2016.06.003

Abstract ( 269 )

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Objective: To investigate the cytocompatibility of ultrasonic micro-arc oxidation-sodium alginate- chitosan composite coating on pure titanium. Methods: The specimens in our research were divided into 3 groups: pure titanium ultrasonic micro-arc-oxidation (group A); pure titanium ultrasonic micro-arc-oxidation chitosan group (group B); and pure titanium ultrasonic micro-arc-oxidation-sodium alginate-chitosan group (group C). Scanning electron microscope was used to observe the surface morphology. CCK 8 experiment was used to detect the adhesion rate and proliferation of osteoblasts. Laser confocal microscopy and scanning electron microscope were used to detect cytoskeleton microfilament stretch and surface morphology of osteoblasts. ALP test was used to evaluate the alkaline phosphatase activity. Results: CCK 8 test showed that, with the extension of time, the OD value of group A, B, and C were increased and in the turn at C>B>A. CCK 8 hyperplastic experiments showed that the proliferation rate of group C and B were better than that of group A. Confocal laser microscope showed the cytoskeleton form. In a certain period of time, the spreading area and the number of microfilament and pseudopodia of group C were the best. The ALP activity was significant higher in group C and in the turn of C>B>A (P<0.05). Conclusion: All three materials showed harmonious cytocompatibility, and the best was the ultrasonic micro-arc oxidation-sodium alginate-chitosan composite coating.

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Therapeutic Effect of Lactoferrin on Traumatic Osteoarthritis Preceded by Osteoporosis.

LI Ji-qing, LIU Yi-ming, ZHU Song-song, LI Yun-feng, HU Jing.

2016, 32(6): 564-569. DOI: 10.13701/j.cnki.kqyxyj.2016.06.004

Abstract ( 340 )

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Objective: To evaluate whether Lactoferrin (LF) has a therapeutic effect on cartilage and subchondral bone in osteoporotic (OP) rats with osteoarthritis (OA). Methods: Forty female Sprague Dawley rats were stratified into four groups with 10 animals per group: OA, OA+LF, OP+OA, OP+OA+LF. OA was induced 12 weeks after bilateral ovariectomy (OVX) or sham operation. LF dissolved in 2ml 0.9% normal saline was administered by oral gavages at a dose of 85mg/kg/d, starting from the first day after the induction of OA. After 8-week treatment, the proximal tibia was harvested for histology, micro-computed tomography (micro-CT), immunohistochemical staining, real-time polymerase chain reaction, as well as western blot evaluation. Results: Therapeutic effect of LF was mainly observed in OVX rats, which increased BV/TV by 30.2%, the Tb.Th by 33.7%, and decreased Tb.Sp by 50.1% in micro-CT analysis of the subchondral bone. LF treatment also decreased the mRNA and protein expression of matrix metalloproteinase-13 (MMP-13) and tartrate-resistant acid phosphatase (TRAP), as well as increasing the mRNA and protein expression of runt-related transcription factor 2 (Runx2) in OVX rats. Conclusion: LF treatment reduced traumatic osteoarthritis in OVX rats, which was possibly related to the beneficial effect of LF on subchondral bone tissue indirectly, or direct effect on cartilage by decreasing the expression of MMP-13 and TRAP.

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Genes Related to Fibroblast Proliferation in Raw 264.7 Cells with Overexpressed Mutant SH3BP2.

ZHAN Fa-long, XU Shu-yu, WANG Hai-cheng, LI Cui-ying, JIANG Jiu-hui, XU Xin.

2016, 32(6): 570-574. DOI: 10.13701/j.cnki.kqyxyj.2016.06.005

Abstract ( 290 )

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Objective: To find genes related to fibroblast proliferation by comparing gene expression profile in RAW 264.7 cells with overexpressed wild type and mutant SH3BP2 gene. Methods: Total RNA in RAW 264.7 cells with overexpressed wild type or mutant SH3BP2 gene were extracted respectively by Trizol and analyzed by microarray to compare differences in gene expression profiles. Database search and real time PCR were performed to verify the most significant related genes in fibroblast proliferation. Results: Microarray data indicated that 734 genes show significant expression differences between two cell lines, among which, 313 genes were up-regulated and 421 down-regulated in cells with mutant SH3BP2 compared with wild type. Following investigation with database search and real time PCR, 4 genes were confirmed the top relevant genes to fibroblast proliferation: CXCL10, IL10, Tnfrsf13b and Pf4. Conclusion: Our study showed expression profiles differences between two cell lines. Four genes were verified to be closely related to fibroblast proliferation and might be the potential key component in the mechanism of Cherubism. This study provides us a bright new window into further study of the disease.

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Effect of Occlusal Traumatism on HMGB1 in the Periodontal Tissues of Ovariectomized Mice: an Immunohistochemical Study.

WEI Jia-qi, LIU Hong-zhi, WANG Tian-xiang, LI Chao.

2016, 32(6): 575-579. DOI: 10.13701/j.cnki.kqyxyj.2016.06.006

Abstract ( 360 )

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Objective: To investigatethe effect of occlusal traumatism on the high mobility group box protein 1(HMGB1) expression in ovariectomized(OVX) mice's periodontal tissues. Methods: Twenty-four female Kunming mice were divided into four groups randomly:①control(CON);②occlusaltraumatism(OT);③ovariectomized(OVX);④ovariectomized+occlusal traumatism(OVX+OT).The mice in OVX and OVX+OT group receivedovariectomy at the beginningof the experiment, and then the OT and OVX+OT were subjected toocclusal traumatism at the 7th week after the surgery. All the mice were killed to make paraffin sections at the 8th week. Immunohistochemistry were applied to study the expression of HMGB1, a computerized image analysis system was used to analyze the result. Results: The HE staining showed that the periodontal membrane fibers in micesubjected toocclusal traumatism were disorganized and torn. More TRAP-positive osteoclasts were found in OVX+OT group than other groups.The expression of HMGB1 in OVX+OT group was the highest.Both TRAP-positive osteoclasts and HMGB1-postive cells in eitherOT or OVX groups were a little more than that in CON group. However there was no significant difference between the two groups. Conclusion: HMGB1 may play a role in the periodontal tissue remodeling of OVX mice subjected to occlusal traumatism.

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Effects of MT01 on the ALP Activity and mRNA Expression of MG63 Infected by Porphyromonas Gingivalis.

GAO Han, SHEN Yu-qin, LIU Yin, HU Tian-qi, FEI Hong-bo, GU Zhong-yi, LI Yang-yang, LIN Chong-tao.

2016, 32(6): 580-583. DOI: 10.13701/j.cnki.kqyxyj.2016.06.007

Abstract ( 206 )

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Objective: To study the effect of MT01 on the differentiation of osteoblasts infected by porphyromonas gingivalis (Pg). Methods: The MG63 cells were seeded into 6- well cell culture plates. MT01 at a final concentration of 1 μg·mL^-1 was added and incubated for 3 hours. Then the cells were challenged by Pg (MOI=100∶1). There were four experimental groups: blank group, MT01, Pg and MT01+Pg. ALP activities in the supernatant fluid and the cell lysate after 24 hours were detected by ALP kit. Real-time PCR was used to detect the mRNA expression of ALP after 2, 4, 6, 8, 12, and 24 hours. Results: MT01 enhanced the ALP activity in infected or uninfected conditions. The mRNA expression of ALP in MG63 was upregulated by MT01 post-infected by Pg or not in a time-dependent manner. Conclusion: MT01 can upregulate the mRNA expression of ALP in Pg infected or uninfected MG63 and improve the ALP activity.

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Effect of Caspase-3/8 on Apoptosis of Human Gingival Mesenchymal Stem Cells under Inflammation.

ZHANG Yang, LIU Jie, SHI Hai-gang, LI Xiao-guang, ZHANG Wei, LIU Na1, XU Lu-lu.

2016, 32(6): 584-587. DOI: 10.13701/j.cnki.kqyxyj.2016.06.008

Abstract ( 215 )

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Objective: To explore the the effect of Caspase-3/8 on apoptosis of human gingival mesenchymal stem cells under inflammation. Methods: Gingival tissues with or without inflammation were obtained from the third impacted molars and gingival hyperplasia because of orthodontic treatment, respectively. Patients were all about 20-25 years old. Normal gingival mesenchymal stem cells (NGMSCs) and inflammatory gingival mesenchymal stem cells (IGMSCs) were isolated and cultured. Apoptosis rates of IGMSCs and NGMSCs were determined by V-FITC/PI Annexin double staining. Caspase-3 and Caspase-8 expression differences were detected by Real-Time PCR. Results: Apoptosis rate of IGMSCs (10.44%) was significantly more than that of NGMSCs (2.76%). Expressions of Caspase-3 and Caspase-8 in IGMSCs group were significantly higher than those of NGMSCs group (P<0.01) as well as TNF- α and IL-1β gene expressions. Conclusion: Caspase-3/8 plays an important role in regulating the proliferation and apoptosis of mesenchymal stem cells.

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Bioinformatic Analysis of TP63-related Proteins

BAI Zhong-cheng, WANG Xin, YIN Wei, BIAN Zhuan.

2016, 32(6): 588-591. DOI: 10.13701/j.cnki.kqyxyj.2016.06.009

Abstract ( 257 )

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Objective: To analyze the interactive molecules of TP63 which is related to hypodontia and cleft lip/palate. Methods: The interactive molecules of TP63 were predicted by the STRING database. The molecular biology function was determined by the DAVID database. Results: The STRING database predicted 50 genes that correlated with TP63. Most of them were involved in the cell proliferation and apoptosis process. Conclusion: Bioinformatic analysis tentatively explored the interactive network of TP63 protein.

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Correlation between the Number of Teeth Missing and Carotid Artery Lesions in Patients with Coronary Heart Disease

XU cai-xia, WANG bing-na, CHEN qing, SHI xue-xue, MA qian-hui, REN xiu-yun.

2016, 32(6): 592-595. DOI: 10.13701/j.cnki.kqyxyj.2016.06.010

Abstract ( 227 )

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Objective: To explore the correlation between periodontitis and atherosclerosis by taking the questionnaire survey and oral examination in patients with coronary heart disease (CHD). Methods: Demographic characteristics, blood pressure, the history of diabetes and cardiovascular disease, lipid series results and oral situation of patients with coronary heart disease were recorded. The carotid artery intima-media thickness (CIMT) and atheromatous plaques were detected by carotid ultrasound. Results: The number of teeth missing was positively correlated with occurrence rate of plaques (OR=1.298, 95%CI: 1.100-1.531, P=0.002). There was significant linear correlation between the number of teeth missing and CIMT (b=0.041). The correlation between the number of teeth missing and the diameter of the plaque was statistically significant(r=0.519, P<0.001). There was no significant difference between the number of teeth missing and levels of blood lipid (P>0.05). Conclusion: It is suggested that teeth missing caused by periodontitis might be a risk factor for atherosclerosis.

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Tooth Movement Tendency with Different Traction Positions in Lingual Orthodontics: A 3-Dimensional Finite Element Analysis.

GAO Xue, ZHANG Chun-zhi, ZHANG Dong-liang.

2016, 32(6): 596-600. DOI: 10.13701/j.cnki.kqyxyj.2016.06.011

Abstract ( 234 )

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Objective: To analyze the tooth movement tendency during anterior retraction with micro implant and sliding method in the customized lingual appliance when traction position is different. Methods: An entire 3-D finite element (FEM) model was constructed, including dentition, periodontal ligament (PDL), alveolar bone, and customized lingual appliance. Using this 3-D FEM model, at different traction positions, mesial or distal to the canine, the anterior teeth and posterior teeth movement tendency during anterior retraction in three dimensions was analyzed. Results: When the lingual anterior retraction hooks (LARHs) were placed distal to the canine, the retraction amount in anterior teeth was more uniformly distributed, dentition constriction appeared in canine and second premolar area, whereas the vertical "bowing effect" was more obvious. Conclusion: During space closure by customized lingual appliance, it is better to locate LARHs distal to the canine. In this way, the decrease of intercanine and interpremolar dental arch width counteracts transverse "bowing effect" effectively. Due to the obvious "bowing effect" in vertical, 10 degrees reverse curve arch wire is necessary to counteract the side effect.

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Research on the Periodontal Treatment Timing for Combined Periodontal-endodontic Lesions

ZHOU Wei, CHEN Bin, ZHAO Jun-jie, LI Hou-xuan, YAN Fu-hua.

2016, 32(6): 601-604. DOI: 10.13701/j.cnki.kqyxyj.2016.06.012

Abstract ( 400 )

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Objective: To explore the periodontal treatment timing for combined periodontal-endodontic lesions. Methods: One hundred and thirty-eight patients with combined periodontal-endodontic lesions, from October 2013 to October 2015 in Nanjing Stomatological Hospital, were randomly divided into control group (group A) and the test group (group B). The involved teeth were treated with root canal therapy (RCT) and full mouth supragingival scaling, and then the subgingival scaling and root planning (SRP) were performed 1 week (group A) or 4-6 weeks (group B) later. SBI, PD, AL, PLI, and TM were recorded at baseline, 2 months, 3 months, 6 months and 12 months for statistical analysis. Results: All the indices observed were not significantly different between group A and group B at baseline before RCT. The differences of SBI, PD, TM were statistically significant between two groups before periodontal treatment (P<0.05). When compared with pre-treatment, the difference of the parameters of baseline and of 2 months after periodontal treatment was statistically significant in both groups. However, there were no statistically significant of the parameters at maintenance periods of 3 months, 6 months, and 12 months when compared to 2 months after treatment. There was no significant difference between two groups at any time point (P<0.05). Conclusion: SRP was suggested to perform 1 week after RCT.

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Expression of MMP-8 and MMP-13 on Mast Cells in Human Chronic Apical Periodontitis

FAN Wan-ting, ZHANG zheng, LI Juan, HUANG Shi-guang.

2016, 32(6): 605-609. DOI: 10.13701/j.cnki.kqyxyj.2016.06.013

Abstract ( 351 )

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Objective: To identify and quantify the expression of MMP-8 and MMP-13 on mast cells (MCs) in different types of human chronic apical periodontitis tissues using double immunofluorescence staining. Mehtods: The specimens (n=90), including healthy control (n=30), periapical cyst (n=30) and periapical granuloma (n=30), were involved in the present study. The tissue samples were fixed in 10% buffered formalin for at least 48 hours and then embedded in paraffin. Serial 5-μm-thick sections were deposited onto SuperFrost/Plus microscope glasses. Routine staining of the sections using hematoxylin & eosin (HE) was performed for morphological evaluation. The number of MMP-8 and MMP-13 positive MCs was identified by double immunofluorescence staining. Results: The density of MMP-8 and MMP-13 positive MCs in the periapical lesions was obviously higher than that in the healthy controls (P<0.01). However, no significant difference of the density of MMP-8 and MMP-13 positive MCs between periapical cyst and periapical granuloma was observed. Conclusion: There is significantly increased number of MCs, along with increased density of MMP-8 and MMP-13 positive MCs in human periapical lesions, which suggests that MMP-8 and MMP-13 positive MCs may play an important role in the pathogenesis of human periapical diseases.

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Effect of Orthodontic Traction for Impacted Maxillary Central Incisor on Root Development

XIAO Hui, LI Lin, LIU Jun-feng, LIU Cong-hua.

2016, 32(6): 610-612. DOI: 10.13701/j.cnki.kqyxyj.2016.06.014

Abstract ( 301 )

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Objective: To evaluate the effect of orthodontic traction for impacted maxillary central incisor on root development. Methods: Patients with impacted maxillary central incisors in different growth periods were treated with orthodontic traction. MCT was used to locate and follow up the growth of the roots. The root development was compared. Results: After traction, 17 incisor roots with bending in growth period continued development in correct direction, with normal crown root ratio, but root length was shorter than the contralateral counterpart teeth. Two incisor roots were bended with defect of alveolar bone which led to traction failure. Conclusion: Early diagnosis and early traction are beneficial to the further development of impacted incisor root, but the defect of alveolar bone should be carefully evaluated.

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Investigation on the Cleaning Capability of MTAN to Post Space Walls.

ZHOU Lin, TONG Xue-mei, CHU Man-ru, ZHANG Qiu-xia.

2016, 32(6): 613-616. DOI: 10.13701/j.cnki.kqyxyj.2016.06.015

Abstract ( 218 )

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Objective: To investigate the cleaning capability of MTAN to the smear layer of post space walls after instrumentation. Methods: After screening, forty newly extracted premolars with single root canal were collected, and the crown parts of which were cut off above 2mm of enamelo-cemental junction. The root canals were shaped and obturated. Post space were prepared and divided randomly into 5 groups, and then irrigated by different solution: 10 ml of 0.9% saline (group A); 2.5 ml of 3% sodium hypochlorite followed by 2.5ml of 18% EDTA solution (group B); 5 ml of 3% sodium hypochlorite followed by 5ml of 18%EDTA solution (group C); 5ml of MTAN solution (group D); 10ml of MTAN solution (group E). All the samples were irrigated by 10 ml of saline ultimately. The morphology of post space was observed under field emission scanning electron microscope (FE-SEM), and scored by Peters’ criterion. Results: The smear layer existed in group A; a large amounts of thick smear layer were present and more dentin tubules were obturated in group B; the distribution of smear layer was up to 50% and the dentinal tubules were partly visible in group C; the smear layer was mostly removed in group D and E, and most of the dentinal tubules were open. Conclusion: MATN is effective to remove the smear layer after post space instrumentation.

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Influence of Potassium Mixing Nano-Hydroxyapatite on the Denfin Bond Strength.

HONG Le-guan, GUO Li-ping, YIN Lu.

2016, 32(6): 617-621. DOI: 10.13701/j.cnki.kqyxyj.2016.06.016

Abstract ( 194 )

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Objective: To observe the change of contact angle on dentine surface with the application of potassium mixing nano-hydroxyapatite, and evaluate the effect of potassium mixing nano-hydroxyapatite on the microleakage of Zinc polycarboxylate cements (ZPCC). Methods: The potassium mixing nano-hydroxy-apatite (K-nHA), nano-hydrxyapatite (nHA), and Gluma desensitizer (Gluma) were used on the prepared human molar teeth respectively. Changes of contact angle on the dentinal surface were observed. Pure titanium crowns were manufactured for each prepared teeth with the previous application of K-nHA, nHA, Gluma and cemented with ZPCC. Microleakage was measured by sliver-staining method and data were analyzed by SPSS17.0 statistics software. Results: The contact angle of K-nHA group was lower than those of other three groups and had a statistically difference between each other. Microleakage value(0.839±0.08)mm in K-nHA group was significantly lower than the blank group (0.966±0.09)mm. Microleakage of nHA group (0.920±0.11)mm and Gluma group (0.977±0.09)mm had no notable statistic difference when compared to the blank group. There was no notable statistic difference in microleakage between the K-nHA group and nHA group. Conclusion: K-nHA is a good hydrophilic materials and its contact angle on dentin surface is minimum. K-nHA has a better sealing function which can significantly reduce microleakage.

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Effect of DEMPs on Proliferation and Differentiation of SHED in Vitro.

ZHANG Yu-na, ZHANG Qi, NI Shi-lei, ZHAO Jing, GAO Xue-bin, HUANG Yang, SUN Hong-chen.

2016, 32(6): 622-626. DOI: 10.13701/j.cnki.kqyxyj.2016.06.017

Abstract ( 213 )

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Objective: To investigate the effect of DEMPs on proliferation and differentiation of SHED in vitro and to provide theoretical basis for the application of DEMPs in tooth reparation and regeneration. Methods: The stem cells were collected from human exfoliated deciduous teeth by enzyme digestion and tissue ex-plant method. We assessed the multi-differentiation potentials of the cells by inducement. Healthy teeth wereextracted from cattles, and prepared dentine matrix of the teeth was extracted by 4mol/L guanidine hydrochloride and 0.5mol/L EDTA. The effect of DEMPs on the proliferation activity of SHED was evaluated by MTT method and the effect ofDEMPs on the activity of ALP was also examined. The expression of DMP-1 and DSPPinduced by DEMPs observed by RT-PCR. Results: The proliferation of SHED was induced by DEMPs after three and five days, and increased activity of ALP was induced by DEMPs after five and seven days. The mRNA expression of DSPP and DMP-1 in SHED induced by DEMPs. Conclusion: DEMPs can promote the proliferation and the odontogenic differentiation of SHED.

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Effects of Periodontitis on Oral Health Related Quality of Life in Elderly Diabetics.

LIU Wen-jing, NIU Fang-qiao, HU Yi, LI Gang.

2016, 32(6): 627-630. DOI: 10.13701/j.cnki.kqyxyj.2016.06.018

Abstract ( 270 )

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Objective: To explore the effect of periodontitis on oral health-related quality of life in elderly diabetics, and to provide a theoretical basis for improving their oral health related quality of life. Methods: 149 type 2 diabetics over the age of 60 were included. Data were collected by the dentist through questionnaires and periodontal health examination. The information was analyzed with software such as Foxpro 6.0 and SPSS 19.0. Results: The total score of OHIP-14 in diabetics with periodontitis was P₂₅=13,P₅₀=16,P₇₅=18, the total score of OHIP-14 in diabetics without periodontitis was P₂₅=7,P₅₀=9,P₇₅=11. The difference between two groups was statistically significant (P<0.05). Both groups had the highest score in the domains of functional limitation and physical pain. Scores of diabetics with periodontitis were higher than scores of diabetics without periodontitis except in the physical disability domain (P<0.05). Conclusion: Periodontitis affects oral health related quality of life in the elderly diabetics, especially in the domain of functional limitation and physical pain. Doctors should pay attention to the periodontal status of elderly diabetics, in order to improve their oral health related quality of life.

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Effects of Marsupialization on Periodontal Bone Regeneration of Involved Teeth in Keratocystic Odontogenic Tumor.

WU Yun-hui.

2016, 32(6): 631-634. DOI: 10.13701/j.cnki.kqyxyj.2016.06.019

Abstract ( 224 )

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Objective: To evaluate the bone regeneration around the involved teeth in keratocystic odontogenic tumor (KCOT) after marsupialization. Methods: 12 cases with large mandibular KCOT were treated with enucleation and marsupialization. The area of KCOT, root length of involved teeth exposed to the cavity and thickness of peri-apical new bone were measured using panoramic and CBCT. The relationship between reduction of KCOT lesion's area and thickness of peri-apical new bone was evaluated by Pearson correlation analysis every three months. Results: 56 teeth were exposed to the cavity and average ratio of the root region covered by periodontal bone was 63.69% before marsupialization. In addition, 49 teeth were completely covered by new bone, and the roots of the other 7 involved teeth were partially covered by new bone with the ratio of 80.02%. The average ratio of root region covered by new bone was 97.5% in all the involved teeth. The average area of the cavity every 3 months was decreased by 2.23±0.94 cm², and the average thickness of peri-apical new bone every 3 months was increased by 1.57±0.72 mm. Meanwhile, the decreased area and increased thickness showed a significant correlation (r=0.908). Conclusion: Marsupialization could effectively promote the new bone formation to cover the peri-apical region of involved teeth.

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Propofol Plus Hydromorphone for Postoperative Patients with Tracheostomy Tube in Oral and Maxillofacial Surgery: a Clinical Study.

PENG Wei, LIU Ke-bin, ZHANG Tie-jun.

2016, 32(6): 635-638. DOI: 10.13701/j.cnki.kqyxyj.2016.06.020

Abstract ( 198 )

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Objective: To investigate sedative, analgesic effects of propofol plus hydromorphone given to postoperative patients with tracheostomy tube in oral and maxillofacial surgery. Methods: Forty patients with endotracheal tube after surgical treatment in oral and maxillofacial surgery were randomly divided into P-H group (propofol plus hydromorphone), P-D group (propofol plus dexmedetomidnie). P-H group received propofol 0.5-1.0 mg/kg/h intravenous infusion plus hydromorphone 4-8μg/kg/h, and P-D group received propofol 0.5-1.0 mg/kg/h intravenous infusion plus dexmedetomidnie 0.2-0.6μg/kg/h. Ramsay score, heart rate, blood pressure, respiratory and other changes were compared between two groups. Time check points included before administration (T0), 30 minutes after administration (T1), 1 hour after administration (T2), 2 hours after administration (T3), 6 hours after administration (T4), 12 hours after administration (T5) and 10 minutes after extubation (T6). The data were processed with SPSS 16.0 software package. Results: There was no significant difference for arterial blood gas analysis, oxygen saturation, mean arterial pressure, respiratory rate and ramsay score between two groups at all time points. However,the changes of heart rate were significantly different between the two groups at T2,T5 and T6. Conclusion: Similar sedative and analgesic effects can be gained in treating postoperative patients with tracheostomy tube in oral and maxillofacial surgery with P-H compared to with P-D. Besides, the former does not result in bradycardia.

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Relationship between Occlusal Tooth Wear and Chewing Side Preference and Lateral Occlusion.

MENG Meng, CHAI Zhi-guo, XIE Cheng, ZHANG Shao-feng, MA Chu-fan.

2016, 32(6): 639-642. DOI: 10.13701/j.cnki.kqyxyj.2016.06.021

Abstract ( 202 )

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Objective:To investigate the relationship between tooth wear and chewing side preference as well as lateral occlusion. Methods: The data were collected by a questionnaire survey and clinical examination. Then t-test was applied to analyze the relationship between occlusal tooth wear and chewing side and preference as well as lateral occlusion. Results: There were 133 subjects (52%) who reported a chewing side preference: 77 (30%) for the right side and 56 (22%) for the left in all of 257 subjects. Chewing side preference was significantly correlated with OTWI scores (P<0.05). If analyzed for the different dental regions separately, the significant difference of OTWI score only existed in the molar region (P<0.05). Of all subjects, 23 (8.9%) had canine protected guidance, 213 (82.9%) had group function, 21 (8.6%) were not measurable at the left side. And 22 (8.6%) subjects had canine protected guidance, 217 (84.4%) had group function, 18 (7.0%) were not measurable at the right side. Subjects with group function had higher mean OTWI scores than those with canine protected guidance in the molar region (P<0.05). Conclusion:Occlusal tooth wear of the chewing side preference was severer than the other side. The molar region occlusal tooth wear of group function was severer than that of canine protected guidance in the molar region.

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Two Minimally Invasive Maxillary Sinus Lifting and Simultaneously Placement of Implants from the Top of Alveoli with 3~5 Years Clinic follow-up Study.

GAO Wei, LI Liang-yu, ZHANG Feng.

2016, 32(6): 643-647. DOI: 10.13701/j.cnki.kqyxyj.2016.06.022

Abstract ( 216 )

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Objective: To compare 2 minimally invasive treatment procedures for maxillary sinus lifting and simultaneously placement of implants. Methods: Seventy cases (81 implants) underwent sinus floor bone graft and simultaneously placement surgery. The mean time of follow up was 36.78±13.74 months. Results: There were 2 implants loose or lost, no clinical complaint of the maxillary sinus area, and X-ray examination of 79 implants showed well osseointegration in 6 to 72 (36.78 ± 13.74) months postoperatively. Conclusion: When residual alveolar bone height was 6-8mm, no significant difference in bone loss was recorded between two minimally invasive applications. While residual alveolar bone height was 2-5mm, a simplified surgical procedure for accessing the maxillary sinus via crestal approach by DASK tools could be adopted. It made the patients less pain and higher satisfaction.

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Inflammatory Myofibroblastic Tumor of Parotid Gland: Analysis of 6 Cases.

ZHANG Chen-yu, WEN Wei-sheng, TAO Ye.

2016, 32(6): 648-651. DOI: 10.13701/j.cnki.kqyxyj.2016.06.023

Abstract ( 267 )

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Objective: To investigate the clinical manifestation, pathological characteristics, diagnosis, therapy and prognosis of Inflammatory myofibroblastic tumor (IMT) of parotid gland. Methods: Six cases of IMT of parotid gland treated in our hospital from 2011 to 2015 were reviewed and analyzed. Results: Six patients, composed of 3 male and 3 female (age 6-75 years), had the IMT of parotid gland in superficial lobe. All the patients were treated by surgery, while 2 of them received hormone therapy after surgery. The patients were followed up for 2-5 years with no recurrence. Conclusion: IMT is a rare benign neoplasm. It is composed of large amounts of spindle cells and infiltrating inflammatory cells such as lymphocytes and plasma cells. IMT of parotid gland is more rare and easy to misdiagnose. Diagnose is relied on the pathology. Treatment is mostly relied on surgery with supplementary hormonotherapy. Prognosis is good and recurrence rate is relatively low

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Clinical Observation of the Efficiency of Mini-Invasive Method for Caries Removal.

HUA Zhi-xiang, CAI Chuan-bao.

2016, 32(6): 652-653. DOI: 10.13701/j.cnki.kqyxyj.2016.06.024

Abstract ( 273 )

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Objective: To investigate the efficiency of caries removal by mini-invasive method using carisolv. Methods: Seventy-six patients with carious lesions were devided randomly into two groups: mini-invasive (carisolv) group and control group, both of which had 38 cases. Carious lesion was removed by mini-invasive method or traditional high speed drilling respectively. The intensity of pain and clinical efficiency of the two groups were observed and analyzed. Results: Patients in the mini-invasive group felt less pain and got better clinical effect than the control group,and the results had l significant differences. Conclusion: Mini-invasive method of caries removal was found more safe and efficient than traditional drilling. The mini-invasive method can be widely used in clinic.

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Research progress on dentine as bone grafting material.

SHENG Ding,ZHONG Wei-jian.

2016, 32(6): 656-658. DOI: 10.13701/j.cnki.kqyxyj.2016.06.026

Abstract ( 296 )

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Jaw bone defects may lead to the functional and morphological abnormality, and make restoration of dental implants more difficult. To avoid secondary injury caused by harvesting of autologous bone, seeking a superior bone substitute material is a focus of present researchers. Dentine has been paid attention in recent years because of its merits of easy availability, simple preparation and reliable bone formation effects. This paper reviewed the mechanism of bone formation, preparation methods, characteristics and clinical application of dentine used as bone grafting material.

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Role of RANK-RANKL-OPG Signaling Pathway in Bone Remodeling

LI Xue, ZHOU Yan-min, MA Shan-shan, MA Yao, GUO Tian-qi, XU Zhao-nan.

2016, 32(6): 659-671. DOI: 10.13701/j.cnki.kqyxyj.2016.06.027

Abstract ( 525 )

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Bone is a dynamic organization which sustains its balance and structural integrity through bone remodeling that composed of bone resorption and bone formation. Receptor activator of nuclear factor kB (RANK)-receptor activator of nuclear factor kB ligand (RANKL)-osteoprotegerin (OPG) has major effects on bone remodeling, especially in the osteoblast lineage to regulate the differentiation and activity of osteoclasts. In addition, the further research of this pathway will have important guiding significance for the clinical treatment of osteoporosis and cardiovascular disease. This article aims to explain the RANK-RANKL-OPG signaling pathway's molecular mechanism in bone.

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