Expression of NLRP3/Caspase-1 Inflammation Pathway in Induced Rat Periapical Lesions.

doi:10.13701/j.cnki.kqyxyj.2016.06.001

Abstract

Abstract: Objective: To investigate the expression of NLRP3/Caspase-1 inflammation pathway in induced rat periapical lesions. Methods: 30 Sprague-Dawley (SD) rats were selected. The pulp cavities of the bilateral mandibular first molars were opened and sealed with phosphate buffer saline (PBS) and glass ionomer. The rats were randomly sacrificed at 0, 7, 14, 21 and 28 days. The mandibles were extracted forhematoxylin-eosin (HE) staining to observe the inflammation conditions in the apical area. The expression of NLRP3, Caspase-1 and IL-1β were examined by immunohistochemistry and real-time PCR. Results: NLRP3, Caspase-1 and IL-1β could be detected in the inflammatory periapical tissues, and the expression were remarkably increased when compared with the control group (P<0.05). The number of Caspase-1 and IL-1β positive cells were significantly correlated with the number of NLRP3 positive cells in the periapical lesions (P<0.01). Conclusion: The expression pattern of NLRP3/Caspase-1 inflammation pathway in experimental periapical lesions suggested that NLRP3-mediated signaling pathway might play an important role in periapical immune defense.

CLC Number:

R781.34

WANG Jiao-jiao, WANG Li-na, YE Dan-dan, AO Xiang, DING Yin-hao, QU Wen-shu, NIU Wei-dong.. Expression of NLRP3/Caspase-1 Inflammation Pathway in Induced Rat Periapical Lesions.[J]. Journal of Oral Science Research, 2016, 32(6): 549-553.

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