Direct Co-culture of Synovial Stem Cells and Chondrocytes Induces Enhanced Cartilage Tissue Formation.

doi:10.13701/j.cnki.kqyxyj.2016.06.002

Abstract

Abstract: Objective: To discuss direct co-culture of rat chondrocytes and synovial-derived mesenchymal stem cells at 1:1 and evaluate chondrocytes induced differentiation of stem cells in vitro. Methods: Rat synovial-derived mesenchymal stem cells and chondrocytes were cultivated in vitro in the same medium. The 3rd generation of synovial-derived mesenchymal stem cells and the 1st generation of chondrocytes in the proportion of 1:1 were mixed. The pellet at the final concentration of 1×10⁶/mL was set as the experimental group, and the considerable population of chondrocytes and synovial-derived mesenchymal stem cells was set as the control group. After cultivated in vitro for 21 days, the newly-born cartilage was evaluated through morphological observation, histological stain, and RT-PCR detection products. Results: After cultivated for 21 days, pellet chondroid tissue was formed and presented tenacious texture and milk white. Type-II collagen was identified by immunohistochemical staining. Matrix could be stained by Type-II collagen. RT-PCR results indicated that pellet expressed cartilage specific genes Type-II collagen and proteoglycan. Conclusion: Through direct co-culture, synovial-derived mesenchymal stem cells and chondrocytes can form mature cartilage.

CLC Number:

R329.2

SHAO Bo, HU Lu-lu, GONG Zhong-cheng, YANG Meng, NING Xiao-ting, WANG Yue, HU Xin, LIU Hui, KEREMU·ABAShi, LIN Bin, YING Xiao-peng, GAO Yun-zu, LIN Zao-quan.. Direct Co-culture of Synovial Stem Cells and Chondrocytes Induces Enhanced Cartilage Tissue Formation.[J]. Journal of Oral Science Research, 2016, 32(6): 554-558.

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