Journal of Oral Science Research

Journal of Oral Science Research ›› 2017, Vol. 33 ›› Issue (4): 367-370.DOI: 10.13701/j.cnki.kqyxyj.2017.04.005

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Effects of Ultrashort Wave on Proliferation and Osteogenic Differentiation of Rat BMSCs.

SONG Yan-yan1, LIU Ling2#, ZHOU Qi-qi1, HAN Xiang-zhen1, ZHAO Xiao-qi1, GE SHU-peng1, HE Hui-yu1*.   

  1. 1. Department of Prosthodontics, the First Affiliated Hospital of Xinjiang Medical University. Urumqi 830054, China;
    2. Department of Rehabilitation Medicine, the First Affiliated Hospital of Xinjiang Medical University. Urumqi 830054, China.
  • Received:2016-09-30 Online:2017-04-20 Published:2017-04-24

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Abstract: Objective: To investigate the effect of ultrashort wave on the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs). Methods: rBMSCs were isolated from SD rats and treated by ultrashort wave fields for 15mins/d. Cell proliferation was determined by CCK-8 method after 1, 4 and 7d respectively. Intracellular alkaline phosphatase (ALP) activity was measured by BCTP/NBT kit after the cells were cultured for 3d. Real-time PCR was used to compare the expression of osteogenesis related gene runt-related transcription factor-2 (RUNX2) after the cells were cultured for 7d. Results: Ultrashort wave significantly enhanced the proliferation (P<0.05) and osteogenic differentiation of rBMSCs, which was indicated by the high ALP production and up-regulated RUNX2 (P＜0.05). Conclusion: Ultrashort wave promoted the proliferation and osteogenic differentiation of rBMSCs.

Key words: Ultrashort wave , BMSCs, Proliferation , Osteogenic differentiation

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