Expression of MMP-8 and MMP-13 on Mast Cells in Human Chronic Apical Periodontitis

doi:10.13701/j.cnki.kqyxyj.2016.06.013

Abstract

Abstract: Objective: To identify and quantify the expression of MMP-8 and MMP-13 on mast cells (MCs) in different types of human chronic apical periodontitis tissues using double immunofluorescence staining. Mehtods: The specimens (n=90), including healthy control (n=30), periapical cyst (n=30) and periapical granuloma (n=30), were involved in the present study. The tissue samples were fixed in 10% buffered formalin for at least 48 hours and then embedded in paraffin. Serial 5-μm-thick sections were deposited onto SuperFrost/Plus microscope glasses. Routine staining of the sections using hematoxylin & eosin (HE) was performed for morphological evaluation. The number of MMP-8 and MMP-13 positive MCs was identified by double immunofluorescence staining. Results: The density of MMP-8 and MMP-13 positive MCs in the periapical lesions was obviously higher than that in the healthy controls (P<0.01). However, no significant difference of the density of MMP-8 and MMP-13 positive MCs between periapical cyst and periapical granuloma was observed. Conclusion: There is significantly increased number of MCs, along with increased density of MMP-8 and MMP-13 positive MCs in human periapical lesions, which suggests that MMP-8 and MMP-13 positive MCs may play an important role in the pathogenesis of human periapical diseases.

CLC Number:

R781.4

FAN Wan-ting, ZHANG zheng, LI Juan, HUANG Shi-guang.. Expression of MMP-8 and MMP-13 on Mast Cells in Human Chronic Apical Periodontitis[J]. Journal of Oral Science Research, 2016, 32(6): 605-609.

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