Effects of Zoledronic Acid on Biological Characteristics of Gingival Fibroblasts

doi:10.13701/j.cnki.kqyxyj.2017.11.009

Abstract

Abstract: Objective: To investigate the effect and mechanism of zoledronic acid on proliferation, apoptosis, and differentiation of gingival fibroblasts. Methods: Primary human gingival fibroblasts (HGFs) isolated from the gingival tissue were treated with 0.5, 1, 5, and 10 μmol/L zoledronic acid for 24, 48, and 72h. Cell proliferation was detected by CCK8 assay. Cell apoptosis was detected by flow cytometry in 10 μmol/L zoledronic acid treated HGFs cells for 72h. Alkaline phosphatase (ALP) activity was detected by ALP kit, the expression of Runx2 and OPN protein were detected by Western blot as well as p65 and IκBα protein after treated with 10 μmol/L zoledronic acid for 72h. Results: Zoledronic acid inhibited proliferation of HGFs in a dose dependent manner (P<0.01). Apoptosis rate of cells in zoledronic acid was significantly higher than that of control group. ALP activity and the expression of Runx2 and OPN protein were significantly higher than those of control group, whereas the protein expressions of p65 and IκBα were significantly lower than the control group (P<0.01). Conclusion: Zoledronic acid can inhibit the proliferation but promote the apoptosis and differentiation of HGFs by downregulating NF-κB signaling pathway.

Key words: Zoledronic acid, Gingival fibroblast, Proliferation, Apoptosis, Differentiation, NF-κB pathway

CLC Number:

R781.4

DONG Wen-jie, WANG Yu-kun, YUE Wen-jiang. Effects of Zoledronic Acid on Biological Characteristics of Gingival Fibroblasts[J]. Journal of Oral Science Research, 2017, 33(11): 1169-1172.

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