Role of C4orf7 in Osteogenic Differentiation of Human Periodontal Ligament Cells

doi:10.13701/j.cnki.kqyxyj.2018.02.005

Abstract

Abstract: Objective: To investigate the effect and potential mechanism of C4orf7 on the osteogenic differentiation of human periodontal ligamentcells (PDLCs). Methods: HE and immunohistochemical (IHC) staining were used to detect the expression of C4orf7 in periodontal tissue. PDLCs were isolated and cultured by enzyme digestion method. Then, PDLCs were transfected with lentiviral vector containing C4orf7 plasmid (LV-c4orf7) or empty vector plasmid (LV-con) and induced inmineralization induction medium for 4,7, and 18 d. Quantification of osteogenic genes (ALP, COL1, RUNX2, and OCN) expression, ALP activity assay, and Alizarin red staining were used to assess the influence of C4orf7 on the osteogenic differentiation of PDLCs. Immunoprecipitation assay was performed to detect the interaction between C4orf7 and BMP2, COLⅠ, and COLⅢ. Results: IHC assay showed that C4orf7 was specifically expressed in periodontal ligament tissue. ALP activity and the mineralization nodules formation were attenuated by C4orf7 overexpression. The mRNA expression levels of osteogenic genes (ALP, COL1, RUNX2, and OCN) were all downregulated in C4orf7 transfected cells. Immunoprecipitation assay indicated that C4orf7 could bind with BMP2 while had no interaction with COLⅠ and COL Ⅲ. Conclusion: C4orf7 may have negative effect on osteogenic differentiation of human PDLCs by interaction with BMP2.

Key words: Periodontal ligament cell, C4orf7, Lentiviral vector transfection, Osteogenic differentiation

CLC Number:

R781.4

WU Yun, ZHAO Yan, JI Yao-ting, XIA Hai-bing, ZHANG Bi, WANG Yi-ning. Role of C4orf7 in Osteogenic Differentiation of Human Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2018, 34(2): 125-129.

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