Calcification of Vascular Smooth Muscle CellInduced by Porphyromonasgingivalis.

doi:10.13701/j.cnki.kqyxyj.2018.02.006

Abstract

Abstract: Objective: To explore the effects of porphyromonasgingivalis(P. gingivalis) on calcification of vascular smooth muscle cell (VSMC). Methods: The primary culture of VSMC was isolated from the aortas of C57BL/6 mice. VSMC was infected with heat-killed P. gingivalis at different MOI. The viability of VSMC was examined after 12, 24, 48 and 72 hours respectively. The calcification and calcium of VSMC were detected after 21 days. Then, P. gingivalis was co-cultured with aorta of mice to investigate the calcification of aorta. Results: VSMC was identified by immunofluorescence staining with specific antibody α-SMA positive. The viability of VSMC stimulated by P. gingivalis(MOI: 10,100) was significantly increased after 24 and 48 hours.All MOI groups enhanced cell viability after72 hours (P<0.05). The calcium content was positively correlated with P. gingivalis MOI (P<0.05). Then, the calcification of aorta induced by P. gingivalis was observed. Conclusion: VSMC viability and calcification areinduced by P. gingivalis, which also results in aortic wall calcification.

Key words: Vascular Calcification, P.gingivalis, VSMC, Periodontitis, Atherosclerosis

CLC Number:

R781.4

TANG Lu, XUE Dong, BAI Yu-hao, WANG Peng-cheng, LI Wen-ting, DONG Yan, ZHOU Wei, ZENG Hong-yan, ZHAO Ying. Calcification of Vascular Smooth Muscle CellInduced by Porphyromonasgingivalis.[J]. Journal of Oral Science Research, 2018, 34(2): 130-133.

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