Study on Anti-inflammatory Effects of PMX205 in Vitro

doi:10.13701/j.cnki.kqyxyj.2017.12.002

Abstract

Abstract: Objective: To explore the anti-inflammatory effects of C5aR antagonist PMX205. Methods: The toxicity of PMX205 for the mouse macrophage (RAW264.7) was tested with MTT assay. RAW264.7 cells were co-cultured with gingipains (G) to simulate the inflammatory environment in vitro and the anti-inflammatory effects of PMX205 was observed. The experiment was divided into six groups: negative control group, G group, and G + PMX205(0.1,1,5,10 mg/L)group. After cultured for 24 h, qRT-PCR and ELISA were used to detect IL-6, TNF-α, IL-10 and TGF-β1. Flow cytometry was used to detect the M1 marker molecule CD86 and M2 marker molecule CD206. Results: MTT results showed that PMX205 had little effect on the viability of RAW264.7. qRT-PCR and ELISA results showed that IL-6 and TNF-α expression levels of G group were higher than the negative control group. The expression of IL-6 and TNF-α were decreased in G + PMX205 group, and TGF-β1 and IL-10 were higher than those in G group. Flow cytometry showed that CD86 positive rate was increased and the CD206 positive rate was decreased in G group. In G+PMX205 group the CD86 was decreased, the CD206 was higher than those in G group. Conclusion: In vitro cell model experiments, PMX205 showed good anti-inflammatory effects

Key words: Periodontitis , Gingipain , PMX205, Macrophage , Polarization

CLC Number:

R781.4

LI Ge-ge, TANG Qiu-ling, PAN Jia-hui, WANG Liu-ran, MENG Yang, YUE Yi-yun, DING Xiao-han, LIU Dong-ning, YU Wei-xian. Study on Anti-inflammatory Effects of PMX205 in Vitro[J]. Journal of Oral Science Research, 2017, 33(12): 1246-1249.

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