Effects of MT01 on the ALP Activity and mRNA Expression of MG63 Infected by Porphyromonas Gingivalis.

doi:10.13701/j.cnki.kqyxyj.2016.06.007

Abstract

Abstract: Objective: To study the effect of MT01 on the differentiation of osteoblasts infected by porphyromonas gingivalis (Pg). Methods: The MG63 cells were seeded into 6- well cell culture plates. MT01 at a final concentration of 1 μg·mL^-1 was added and incubated for 3 hours. Then the cells were challenged by Pg (MOI=100∶1). There were four experimental groups: blank group, MT01, Pg and MT01+Pg. ALP activities in the supernatant fluid and the cell lysate after 24 hours were detected by ALP kit. Real-time PCR was used to detect the mRNA expression of ALP after 2, 4, 6, 8, 12, and 24 hours. Results: MT01 enhanced the ALP activity in infected or uninfected conditions. The mRNA expression of ALP in MG63 was upregulated by MT01 post-infected by Pg or not in a time-dependent manner. Conclusion: MT01 can upregulate the mRNA expression of ALP in Pg infected or uninfected MG63 and improve the ALP activity.

CLC Number:

R781.4

GAO Han, SHEN Yu-qin, LIU Yin, HU Tian-qi, FEI Hong-bo, GU Zhong-yi, LI Yang-yang, LIN Chong-tao.. Effects of MT01 on the ALP Activity and mRNA Expression of MG63 Infected by Porphyromonas Gingivalis.[J]. Journal of Oral Science Research, 2016, 32(6): 580-583.

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