Effect of anti-Streptococcus Mutans Yolk Antibody on Virulence Factors and Plaque Biofilm induced by Streptococcus Mutans.

doi:10.13701/j.cnki.kqyxyj.2019.05.009

Abstract

Abstract: Objective: The anti-Streptococcus mutans egg yolk immunoglobulin (anti-S. mutans-IgY) was prepared and purified, and its effect on the extracellular polysaccharides capacity, glucosyltransferase activity, biofilm formation and activity of S. mutans was observed. Methods: Ten 150 day-old laying hens were immunized with S. mutans and IgY was isolated from the egg yolk by the water dilution (WD) method and 2 steps of ammonium sulfate purification. ELISA was used to detect changes in titer after immunization. The anthrone method was used to observe the water soluble glucan (WSG) and water insoluble glucan (WIG), the Neson-Somogyi method was used to determine the activity of GTF enzyme, and the XTT method was used to study the effect of IgY on the total amount of biofilm. Results: After 4 immunizations, the highest titer of the antibody was 1:256000, which was maintained for 40 days. IgY inhibited the WIG synthesis of S.mutans, and decreased the S.mutans reducing sugar and enzyme activity (P<0.01). The XTT method showed that the inhibition rate of IgY to S. mutans biofilm was17.02%-74.13%. Microscopy showed that the green viable bacteria and structure decreased and the biofilm formation was significantly inhibited as the concentration of the drug solution increased. Conclusion: IgY can inhibit the proliferation of S. mutans and has an anti-caries effect.

Key words: Streptococcus mutans, Immunoglobulin Y, Glucosyltransferase, Water- insoluble glucan, Dental plaque biofilm

ZHOU Li-li, XU Yan, JIANG Peng, XU HAN-ying, XIN Bao-jian, SHEN Ji-long, CHENG Ting. Effect of anti-Streptococcus Mutans Yolk Antibody on Virulence Factors and Plaque Biofilm induced by Streptococcus Mutans.[J]. Journal of Oral Science Research, 2019, 35(5): 448-452.

References

[1] Kaur G, Rajesh S, Princy SA. Plausible drug targets in the Streptococcus mutans, quorum sensing pathways to combat dental biofilms and associated risks [J]. Indian J Microbiol, 2015, 55(4):349-356.
[2] 仇元新,方绍伟,秦宗长.变异链球菌葡萄糖基转移酶转录调控因子的研究进展[J].口腔医学研究,2017,33(7):109-111.
[3] Lei L, Yang Y, Mao M, et al. Modulation of biofilm exopolysaccharides by the Streptococcus mutans vicX gene [J]. Front Microbiol, 2015, 6:1432.
[4] Smith DJ, King WF, Godiska R. Passive transfer of immunoglobulin Y antibody to Streptococcus mutans glucan binding protein B can confer protection against experimental dental caries [J]. Infect Immun, 2001, 69(5):3135-3142.
[5] 王晓静,徐燕,孟明理, 等. 抗中间普氏菌卵黄抗体的制备及其对实验性妊娠期龈炎的预防作用[J].安徽医科大学学报,2017,52(8):1154-1159.
[6] Packiavathy IASV, Agilandeswari P, Musthafa KS, et al. Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolite methyl eugenol against Gram negative bacterial pathogens [J]. Food Res Int, 2012, 45(1):85-92.
[7] Koo H. In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens [J]. Arch Oral Biol, 2000, 45:141-148.
[8] Huang R, Li M, Gregory RL. Effect of nicotine on growth and metabolism of Streptococcus mutans [J]. Eur J Oral Sci, 2012, 120(4):319-325.
[9] Wróblewska M, Struzycka I, Mierzwiñska-Nastalska E. Significance of biofilms in dentistry [J]. Przegl Epidemiol, 2015, 69(4):739-744.
[10] Dongyeop K, Geelsu H, Yuan L, et al. Cranberry flavonoids modulate cariogenic properties of mixed-species biofilm through exopolysaccharides-matrix disruption [J]. PLoS One, 2015, 10(12):e0145844.
[11] Liu S , Wei Y , Zhou X , et al. Function of alanine racemase in the physiological activity and cariogenicity of Streptococcus mutans [J]. Sci Rep, 2018, 8(1):5984.

[1]	ZHAO Xia, XU Jiahao, XIA Shang, ZHANG Chen, WANG Wanchun. Construction of Water-soluble Farnesol Nano-micelle and Its Antibacterial Effect on Streptococcus Mutans [J]. Journal of Oral Science Research, 2020, 36(9): 875-878.
[2]	ZHANG Mengjie, LU Wei, HOU Mengyao, JIANG Xiu, WANG Feihu, TAO Rui. Preliminary Study on Dual-species Biofilm of Enterococcus faecalis and Streptococcus mutans. [J]. Journal of Oral Science Research, 2020, 36(12): 1128-1131.
[3]	HAN Linxiu, WANG Wang, WANG Ling, YANG Weihong,LIU Yao, LIU Xingrong. Effect of HtrA on Expression of gbpC of Streptococcus Mutans Isolated from Children with High Cario-susceptibility. [J]. Journal of Oral Science Research, 2020, 36(1): 61-64.
[4]	GUO Xinwei, ZHAO Hongyan, CHENG Boqun, QIAN Xin, ZHANG Zhimin. Effect of Fluoride-resistant Streptococcus Mutans on Adhesion of CAD/CAM Ceramics to Teeth [J]. Journal of Oral Science Research, 2019, 35(8): 752-756.
[5]	YANG Yao-yao, ZHANG Zhi-min, ZHAO Hong-yan, GUO Xin-wei, LIU Lu, LING Xiao-xu, QU Tian. Effect of Fluoride on Biofilm Formation of Streptococcus Mutans Fluoride-resistant Strains [J]. Journal of Oral Science Research, 2019, 35(6): 546-550.
[6]	QUAN Xu, XU Tong-tong, ZHANG Hui-yan, WANG He-ling, WANG Chun-yang, CUI Yun-xia, FU Qi-yue, MENG Wei-yan.. Effects of D-Histidine on Streptococcus Mutans Biofilm. [J]. Journal of Oral Science Research, 2019, 35(2): 176-179.
[7]	SUN Tong-zheng, YANG Fang. Research Progress in Prediction of Dental Caries Based on FQ-PCR [J]. Journal of Oral Science Research, 2019, 35(1): 13-15.
[8]	KONG Jing-jing, LIANG Xiang-yang, LI Chun-nian, DAI Xin-peng, MA Xiang-tao. Inhibition Effects of Magnolol on Streptococcus Mutans and Demineralized Enamel caused by Carbonated Beverages [J]. Journal of Oral Science Research, 2018, 34(7): 788-792.
[9]	GUO Xiao, QIU Wei, ZHOU Xue-dong, CHENG Lei, LI Ming-yun. Gene Expression, Purification, and Activity Determination of Alanine Racemase from Streptococcus Mutans [J]. Journal of Oral Science Research, 2018, 34(6): 597-600.
[10]	LI Wen-yue, GAO Shuang, ZHANG Zhi-ying, ZHANG Hong, CHAO Bo, WU Yu-feng, LI He, WANG Chun-meng, ZHANG Zhi-min. Construction of Streptococcus Mutans Fluoride-resistant Strain with GtfB Gene Inactive. [J]. Journal of Oral Science Research, 2018, 34(5): 490-494.
[11]	FAN Yue, MENG Xiang-feng. Application of in Situ Synthesized Silver Nanoparticles to Antibacterial Modification of Resin Adhesives [J]. Journal of Oral Science Research, 2018, 34(2): 161-164.
[12]	Cao Xi-xi, Ye Qian-lin, Zhou Li-bo, Fan Ming-wen, Liu Chang. Study on Inhibitory Effect of Ginsenoside Rh2 on Cariogenic Bacteria Biofilms [J]. Journal of Oral Science Research, 2018, 34(12): 1302-1306.
[13]	XU Jing-jing, TAO Ting-liang, LI Yu-hong.. Anti-caries Effect of A Bacteriophage Lysin. [J]. Journal of Oral Science Research, 2018, 34(10): 1052-1056.
[14]	DONG Wei, CHEN Rui, YAN Quan, BAO Guang-jie. Effects of Three Plant Extracts on Cariogenic Tolerance of Streptococcus Mutans [J]. Journal of Oral Science Research, 2017, 33(9): 943-945.
[15]	QIU Yuan-xin, FANG Shao-wei, QIN Zong-chang.. Research Progress of Regulator of Streptococcus Mutans Glucosyltransferase. [J]. Journal of Oral Science Research, 2017, 33(7): 792-794.