Effect of Fluoride-resistant Streptococcus Mutans on Adhesion of CAD/CAM Ceramics to Teeth

doi:10.13701/j.cnki.kqyxyj.2019.08.008

Abstract

Abstract: Objective: To investigate the effect of Streptococcus mutans and its fluoride-resistant strains on the bonding interface between three kinds of ceramics and enamel, dentin, and fluid resin. Methods: Fluoride-resistant strains of Streptococcus mutans were induced. And ceramic-enamel/dentin/fluid resin specimens were prepared. The specimens were immersed in Streptococcus mutans UA159, its fluoride-resistant strain, and BHI culture medium, respectively. The bonding interface was observed under stereomicroscope and electron microscope for 14 days. Results: (1) Ceramic-adhesive interface: the cracks between ceramic and adhesive dipped in UA159 were more than those dipped in fluoride-resistant strains; the cracks between resin-based ceramic Lava Ultimate and adhesive were the most; (2) adhesive-teeth interface: the cracks between adhesive and fluid resin were the least; UA159 and its fluoride-resistant strains had similar influence on the interface. Conclusion: Streptococcus mutans and its fluoride-resistant strains can destroy the interface of ceramic-adhesive-dental tissue, and the most destructive effect is happened in resin-based ceramics.

Key words: Fluoride-resistant Streptococcus mutans, Streptococcus mutans, CAD/CAM, interface destruction

GUO Xinwei, ZHAO Hongyan, CHENG Boqun, QIAN Xin, ZHANG Zhimin. Effect of Fluoride-resistant Streptococcus Mutans on Adhesion of CAD/CAM Ceramics to Teeth[J]. Journal of Oral Science Research, 2019, 35(8): 752-756.

References

[1] Ruse ND, Sadoun MJ. Resin-composite blocks for dental CAD/CAM applications [J]. J Dent Res, 2014, 93(12):1232-1234.
[2] Singh J, Khalichi P, Cvitkovitch DG. Composite resin degradation products from BisGMA monomer modulate the expression of genes associated with biofilm formation and other virulence factors in Streptococcus mutans [J]. J Biomed Mater Res A, 2009, 88(2):551-560.
[3] Bourbia M, Ma D, Cvitkovitch DG, et al. Cariogenic bacteria degrade dental resin composites and adhesives [J]. J Dent Res, 2013, 92(11):989-994.
[4] Huang B, Sadeghinejad L, Adebayo OIA, et al. Gene expression and protein synthesis of esterase from Streptococcus mutans are affected by biodegradation by-product from methacrylate resin composites and adhesives [J]. Acta Biomater, 2018, 81:158-168.
[5] Schmitter M, Seydler B. Minimally invasive lithium disilicate ceramic veneers fabricated using chairside CAD/CAM: a clinical report [J]. J Prosthetic Dent, 2012, 107(2):71-74.
[6] Liao Y, Chen J, Brandt BW, et al. Identification and functional analysis of genome mutations in a fluoride-resistant Streptococcus mutans strain [J]. PLoS One, 2015, 10(4):e0122630.
[7] Capa N, Say EC, Celebi C. Microtensile bond strengths of adhesively bonded polymer-based CAD/CAM materials to dentin [J]. Dent Mater J, 2019, 38(1):75-85.
[8] 汪嘉,何智妍,冉淑君,等.耐氟变形链球菌对树脂-牙本质粘接强度的影响[J].上海口腔医学, 2018, 27(3):239-243.
[9] 王南燕.变形链球菌侵袭对4种复合树脂粘结界面老化的影响[J].口腔医学研究, 2016, 32(1):83-86.
[10] Rosa WL, Piva E, Silva AF. Bond strength of universal adhesives: A systematic review and meta-analysis [J]. J Dent, 2015, 43(7):765-776.
[11] Parlar Oz O, Secilmis A, Aydin C. Effect of laser etching on glass fiber posts cemented with different adhesive systems [J]. Photomed Laser Surg, 2018, 36(1):51-57.
[12] Nedeljkovic I, De Munck J, Ungureanu AA, et al. Biofilm-induced changes to the composite surface [J]. J Dent, 2017, 63(1):36-43.
[13] Shokati B, Tam LE, Santerre JP. Effect of salivary esterase on the integrity and fracture toughn ess of the dentin-resin interface [J]. J Biomed Mater Res B Appl Biomater, 2010, 94(1):230-237.
[14] Jaffer F, Finer Y, Santerre JP. Interactions between resin monomers and commercial composite resins with human saliva derived esterases [J]. J Dent Res, 2013, 92(11):989-994.

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