IGF1-PI3K/mTOR Pathway Has Regulatory Effects on Cytokines in T cells and Keratinocytes Co-culture System

doi:10.13701/j.cnki.kqyxyj.2019.04.022

Abstract

Abstract: Objective: To investigate the influence of IGF1-PI3K/MTOR signaling pathway, which was abnormally activated in OLP, on the inflammatory cytokines in the interaction of T cells and keratinocytes in oral lichen planus (OLP). Methods: A transwell co-culture system composed of T cells and oral keratinocytes was used as a model of OLP in vitro. The effects of activated T cells pretreated with LY294002, rapamycin, and IGF1 on the levels of IL-2 and TNF-α in supernatant from T cell alone and co-culture system were detected via ELISA, respectively. Results: Compared to the T cells cultured alone, the interaction of keratinocytes and T cells could increase the secretion of TNF-α (P<0.05), but has no impact on the level of IL-2. Whereas the aberrant IGF1-PI3K/MTOR signaling decreased the IL-2 and TNF-α secretion in the co-culture system (P<0.05). Conclusion: The IGF1-PI3K/MTOR signaling may modulate the cytokine networks in the crosstalk between T cells and keratinocytes and participate in the progression of OLP.

Key words: Oral lichen planus, T cells, Keratinocytes, IGF-PI3K/MTOR pathway

MA Rui-jie, TAN Ya-qin, ZHOU Gang. IGF1-PI3K/mTOR Pathway Has Regulatory Effects on Cytokines in T cells and Keratinocytes Co-culture System[J]. Journal of Oral Science Research, 2019, 35(4): 397-400.

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