Effects of Bone Marrow Mesenchymal Stem Cells Derived Exosomes on Proliferation and Differentiation of Osteoblasts

doi:10.13701/j.cnki.kqyxyj.2019.04.023

Abstract

Abstract: Objective: To evaluate the effects of bone marrow mesenchymal stem cells (BMSCs) derived exosomes on the proliferation and differentiation of osteoblasts. Methods: BMSCs were cultured in vitro and exsomes were isolated by ultracentrifugation from supernatants. After treated with BMSCs derived exosomes, the proliferation of osteoblasts was detected by CCK-8 assay. Alkaline phosphatase activity (ALP) analysis and alizarin red staining were adopted to evaluate the effect of BMSCs derived exosomes on mineralization process of osteoblasts. Real-time PCR was utilized to detect the expression of odontogenesis-related genes. Results: BMSCs derived exosomes enhanced the proliferation of osteoblasts and upregulated the expression levels of odontogenesis-related genes. The ALP activity and calcium accumulation of osteoblasts were increased significantly as well. Conclusion: BMSCs derived exosomes could promote the proliferation and differentiation of osteoblasts.

Key words: Bone marrow mesenchymal stem cells, Exosome, Osteoblast, Proliferation, Differentiation

CHEN Yan, JIANG Sheng-jun, PENG You-jian. Effects of Bone Marrow Mesenchymal Stem Cells Derived Exosomes on Proliferation and Differentiation of Osteoblasts[J]. Journal of Oral Science Research, 2019, 35(4): 401-404.

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