Effect of In Vitro Indirect Co-culture of Rabbit Dental Pulp Stem Cells with Schwann Cells on Proliferation and Neuronal Differentiation of Rabbit Dental Pulp Stem Cells

doi:10.13701/j.cnki.kqyxyj.2020.10.018

Abstract

Abstract: Objective: To study the effects of indirect co-culture of rabbit dental pulp stem cells (rDPSCs) and rabbit schwann cells (rSCs) in vitro on each other's proliferation and the possibility of rSCs inducing rDPSCs into neural differentiation. Methods: The indirect co-culture system of rSCs/rDPSCs was constructed by Transwell indirect co-culture system, and MTT method was adopted to detect the cells' proliferation. Immunofluorescence staining and Western bolt were used to study the expression of S-100β and NF-H and the possibility of rSCs to induce neural differentiation of rDPSCs. Results: When rDPSCs and rSCs were indirectly co-cultured in a 1∶2 ratio for 5 days, rDPSCs could facilitate the proliferation of rSCs (P<0.05), and rSCs also had obvious effect on the proliferation of rDPSCs (P<0.05). After indirect co-culturet with rSCs for 7 days and 14 days, rDPSCs became neuron-like cells, S-100β and NF-H were expressed and both presented a higher level after 14 days (P<0.01). Conclusion: In the indirect co-culture system of rDPSCs and rSCs, rDPSCs and rSCs can significantly promote each other's proliferation after 5 days, while rSCs can induce the neural differentiation of rDPSCs.

Key words: rabbit dental pulp stem cells, rabbit Schwann cells, transwell, cell proliferation, neural differentiation

ZHANG Weijia, PAN Shuang, LI Yanping, HE Lina, WU Zuodong, NIU Yumei. Effect of In Vitro Indirect Co-culture of Rabbit Dental Pulp Stem Cells with Schwann Cells on Proliferation and Neuronal Differentiation of Rabbit Dental Pulp Stem Cells[J]. Journal of Oral Science Research, 2020, 36(10): 968-972.

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