Effect of Silencing Tiam1 Gene by Small RNA Interference on Biological Characteristics of Tongue Squamous Cell Carcinoma Cells

doi:10.13701/j.cnki.kqyxyj.2017.09.010

Abstract

Abstract: Objective: To investigate the effect of silencing Tiam1 gene by small RNA interference (siRNA) on the biological characteristics of tongue squamous cell carcinoma cells. Methods: The human tongue cancer cells of SCC-4 were cultured. All cells were randomly divided into siRNA-Tiam1 group, siRNA-control sequence group, and blank control group. The expressions of Tiam1 gene and protein in different transfection groups were detected by real-time fluorescence quantitative PCR and Western blot, respectively. The cell proliferations in different transfection groups were detected by MTT assay. The cell apoptosis in different transfection groups were detected by flow cytometry. The cell migration and invasion in different transfection groups were detected by Transwell assay. Results: The relative expression levels of Tiam1 gene and protein in the siRNA-Tiam1 group were lower than those in the siRNA-control sequence group and blank control group (P<0.05). MTT assay showed that, compared with siRNA-control sequence group and blank control group, the absorbance A values at 24h, 48h, 72h and 96h in the siRNA-Tiam1 group decreased (P<0.05). Flow cytometry showed that the apoptosis rate in the siRNA-Tiam1 group was significantly higher than that in the siRNA-control sequence group and blank control group (P<0.05). The number of migrating cells and invasive cells in the siRNA-Tiam1 group was lower than that in the siRNA-control sequence group and blank control group (P<0.05). Conclusion: Specific silencing Tiam1 gene could effectively inhibit the proliferation of tongue squamous cell carcinoma cells, accelerate cell apoptosis, and inhibit cell migration and invasion ability.

Key words: Tongue squamous cell carcinoma , Small interference RNA , Tiam1 Biological , characteristics

CLC Number:

R739.8

CHEN Hu-jie, DAI De-hua, DAI Jing, WEI Wei, XIN Hai-tao. Effect of Silencing Tiam1 Gene by Small RNA Interference on Biological Characteristics of Tongue Squamous Cell Carcinoma Cells[J]. Journal of Oral Science Research, 2017, 33(9): 950-953.

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