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Design of Sectional Porous Dental Implant Based on Finite Element Analysis YAO Chun-yan, ZHANG Wei, LI Kuang-wei, REN Teng-xi, LIU Yun-feng 2017, 33(9): 913-915.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.001 Abstract ( 255 )   PDF (2042KB) ( 222 )   Objective: To determine the geometric parameters of sectional structure of porous implants based on statistical analysis of ordinary implant, and to compare the advantage of porous implant with different pore shape, pore size and location to the solid implant by finite element analysis. Methods: 6D dental implants design software was used to determine the geometric parameters by building UG models. The largest and suitable occlusal force loading was simulated in the finite element software to analyze stress distribution of four different implants in the mandible. Results: The stress distributions of four different implants in the mandible are different. The stress of diamond staggered arrangement of porous implants is significantly less than others. Conclusion: Compared to solid implant, appropriate porous structure implant is far more superior for implant restoration. References | Related Articles | Metrics

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Effect of Rhodiola Rosea Glycosides on Function of Osteoblasts and Related Molecular Mechanism MEI Guo-hua, GUO Xiao-qin, CHEN Jing-jing, WANG Yue, LI Zheng-yang 2017, 33(9): 916-919.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.002 Abstract ( 303 )   PDF (2887KB) ( 261 )   Objective: To study the effects of salidroside (SAL) on proliferation, differentiation, mineralization, and the expression of VEGF depending on HIF-1α of MG-63 cells and related molecular mechanisms. Methods: To study the effects SAL on proliferation, differentiation, mineralization, the expression of VEGF depending on HIF-1α, and related molecular mechanisms, the cell vitality assay, Flow cytometry analysis, semi-quantitative reverse transcription polymerase chain reaction, ELISA and Western blot were used. Results: SAL promoted the proliferation and differentiation of MG-63 cells through regulating the distribution of cell cycle, the mRNA and protein levels and the expression of HIF-1α. SAL could also increase the VEGF production of downstream target genes of MG-63 cells. Conclusion: In vitro experiment study shows that SAL can promote proliferation, differentiation, mineralization, the production of HIF-1α and downstream target genes VEGF of MG-63 cells to promote the formation of new bones and vessels. References | Related Articles | Metrics

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Effect of New Type Multistage Aperture Bioactive Glasses Prepared by Pollen and P123 Dual Template on Proliferation and Differentiation of MG63 Cells WANG Yang,SHEN Yu-qin,LING Xin-lian,LIU Yu-yan,QIN Tian-tian,DIAO Meng-xue,SUN Xin-hua 2017, 33(9): 920-923.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.003 Abstract ( 241 )   PDF (950KB) ( 221 )   Objective: To investigate the effects of new type multistage aperture bioactive glasses which were prepared by pollen and P123 dual template on the proliferation and differentiation of MG63 cells. Methods: MG63 cells were cultured in the material leach liquor (0.2g/ml working concentration, DMEM with 10% fetal bovine serum) for 1, 3, and 5 days. MTT colorimetric method was used to detect the level of cell proliferation. Alkaline phosphatase kits was used to measure the ALP activity. Real-time PCR was used to analyze the mRNA expression of osteogenesis marker, i.e. RUNX-2, SP7, and COL-1. Results: The material promoted the proliferation of MG63 cells, enhanced the activity of ALP, up-regulated the mRNA expression level of markers gene RUNX-2, SP7, and COL-1. Conclusion: The new type multistage aperture bioactive glasses which were prepared by pollen and P123 dual template has low cytotoxicity and the potential to promote the osteogenetic differentiation. References | Related Articles | Metrics

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Therapeutic Effect of Adenovirus-mediated HGF Transfection of ADSCs Combined with Injectable Hydrogel on the Repair of Temporomandibular Joint Arthritic Condylar Cartilage in Rabbits ZHANG Guang-de, LI Rong-liang, YUE Cong-lei, YANG Shi-mao, GUO Yan-wei, FANG Dian-ji 2017, 33(9): 924-927.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.004 Abstract ( 243 )   PDF (3617KB) ( 330 )   Objective: To investigate the therapeutic effect of Ad-HGF-GFP of ADSCs combined with injectable hydrogel on the repair of damaged cartilage in the microenvironment of rabbit temporomandibular arthritis. Methods: ADSCs were isolated from rabbits and cultured in vitro. Cells were transfected with adenovirus-mediated HGF and injectable hydrogel were prepared. Then animals were randomly divided to five groups: group A (blank control); group B; group C (treated with ADSCs); group D (treated with ADSCs combined with injectable hydrogel); group E (treated with Ad-HGF-GFP-ADSCs combined with injectable hydrogel). All animals were sacrificed 3 or 9 weeks after surgery, and condylar cartilages were harvested and analyzed by scanning electron microscope, histomorphometric analysis, and real-time PCR. Statistical analysis was performed with SPSS 17.0 software and statistically significant differences were assessed by t-test. All statistical analysis was considered significant when P<0.05. Results: Electron microscope scanning and histomorphometric analysis indicated improvement of condylar cartilage in group D and E, compared to those in the group B and C. Real-time PCR showed that the 基金项目 国家自然科学基金(编号:81170935) 作者简介 张广德(1977~ ),男,山东人,学士,主治医师,主要从事口腔颌面外科临床治疗工作。 *通讯作者 房殿吉,E-mail:dianjifang@yahoo.com.cn expression of mmp13 gene in group E and A was comparable but decreased in group C and D, the difference was statistically significant. In contrast, the expression of TIMP-1 gene in group E and A was comparable but increased in group C and D, and the difference was statistically significant. Conclusion: Adenovirus-mediated HGF transfection of ADSCs combined with injectable hydrogel could promote the repair of temporomandibular joint arthritic condylar cartilage in rabbits. References | Related Articles | Metrics

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Role of Micro-34a in Induced-mineralization of Human Periodontal Ligament Cells CHENG Meng-wen, ZHOU Yi 2017, 33(9): 928-932.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.005 Abstract ( 339 )   PDF (1123KB) ( 215 )   Objective: To investigate the effect of miR-34a on the osteogenic differentiation of human periodontal ligament cells (hPDLCs). Methods: HPDLCs were obtained through in vitro culture combined with enzyme digestion method and tissue pieces culture method. First, the cells were continuously induced by mineralization induction medium for 3,7, and 14 days. The expression of miR-34a was detected by quantitative real-time PCR (qRT-PCR). Second, hPDLCs were transfected with lentiviral vector containing pre-miR-34a or control to build the miR-34a overexpression model or control model (hPDLCs/34a or hPDLCs/pCDH) and induced by mineralization induction medium for 3, 7, 14, and 21 days. Quantification of osteogenensis-related genes (ALP, Runx2, OCN and BSP) expression, alkaline phosphatase (ALP) activity detection and Alizarin red staining were used to assess the influence of miR-34a on the osteogenic differentiation of hPDLCs. Results: The expression of miR-34a significantly increased in a time-dependent manner (P<0.001) during the osteogenic differentiation. The mineralization nodule formation and ALP activity were all attenuated by miR-34a. ALP gene expression detected by qRT-PCR was reduced after 7 days' induction in hPDLCs/34a. Runx2, OCN and BSP gene expression showed no significant 基金项目 国家自然科学基金(编号:81200812) 武汉市应用基础研究计划项目(编号:2016060101010043) 作者简介 程孟文(1995~ ),女,安徽阜阳人,硕士在读,主要从事口腔修复学研究工作。 *通讯作者 周毅,E-mail: dryizhou@163.com differences. Conclusion: MiR-34a may have a negative effect on the osteogenic differentiation of hPDLCs. References | Related Articles | Metrics

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Effect of Sandblast and Acid Etching on Attachment, Proliferation, and Differentiation of Osteoblasts on Ti-24Nb-4Zr-7.9Sn. FENG Shuai-ru, LIU Xin, PAN Shuang, LIU Hui-mei, NIU Yu-Mei 2017, 33(9): 933-937.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.006 Abstract ( 235 )   PDF (2615KB) ( 274 )   Objective: To evaluate the effect of sandblast and acid etching treatments on attachment, proliferation, and differentiation of osteoblasts on Ti and Ti-24Nb-4Zr-7.9Sn (TNZS). Methods: Samples were divided into four groups: Ti group, Ti-SLA group, TNZS group, and TNZS-SLA group. Ti and TNZS groups were polished with different rough emery papers, Ti-SLA and TNZS-SLA groups were polished with different rough emery papers and sandblasted by Al2O3 particles and acid etched by mixture of hydrochloric acid and sulfuric acid. Surface morphology of the samples was analyzed by scanning electron microscope (SEM). Contact angles were examined by Tensiometer and Contact Angle Meter. Roughness of samples was examined by 3D laser scanning confocal microscope. MC3T3-E1 were seeded on the surface of four groups of samples and evaluated by different means. Results: Ti-SLA and TNZS-SLA surfaces exhibited micro-topographical and nano-topographical modifications and roughness distributed evenly. After treatment, four groups had similar hydrophilicity. The adhesion of MC3T3-E1 preosteoblasts showed that Ti and TNZS had higher attachment than SLA groups (P<0.05). TNZS-SLA showed the highest proliferation and differentiation (P<0.05). Conclusion: SLA surface treatment generates porous microstructures and nanostructures. It can enhance proliferation and differentiation of osteoblasts. TNZS alloy treated by sandblast and acid etching possesses good cytocompatibility. References | Related Articles | Metrics

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Role of PI3K/AKT/mTOR/p70S6K Pathway in Drug Resistance of Tongue Squamous Cells ZHANG Si-ying, PAN Shu-ting, SHUAI Fang-yuan, QIU Jia-xuan 2017, 33(9): 938-942.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.007 Abstract ( 276 )   PDF (6555KB) ( 319 )   Objective: To investigate the role of PI3K/AKT/mTOR/p70S6K signaling pathway in drug resistance of tongue squamous cells. Methods: Tongue squamous cell carcinoma line Cal27 and tongue squamous cell carcinoma cell line Cal27/CDDP with cisplatin resistance were repeatedly treated with the specific inhibitor, LY294002 (PI3K/AKT inhibitor) , Rapamycin (mTOR inhibitor), and LY2584702 (p70S6K inhibitor). Related proteins in PI3K/AKT/mTOR/p70S6K pathway were detected by Western blot. The level of autophagy was detected with Cyto-ID fluorescence staining. Cellular apoptosis was detected by flow cytometry. Results: After applying the pathway inhibitors, the expression level of Beclin1 of the tongue squamous cells were enhanced compared with the control group. Moreover, the ratio of the LC3II to LC3I and the ratio of the Bax to Bcl-2 were increased. The expression of p-AKT, p-mTOR, and p-p70S6K were decreased (P<0.05). The apoptosis and autophagy level were increased in tongue squamous cells, compared with control group. Conclusion: Inhibiting the PI3K/AKT/mTOR/p70S6K signaling pathway shows a significant effect on apoptosis and autophagy in Cal27 and Cal27/CDDP. References | Related Articles | Metrics

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Effects of Three Plant Extracts on Cariogenic Tolerance of Streptococcus Mutans DONG Wei, CHEN Rui, YAN Quan, BAO Guang-jie 2017, 33(9): 943-945.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.008 Abstract ( 325 )   PDF (783KB) ( 290 )   Objective: To study the effects of green tea, wild chrysanthemum, mint extract and their compound on the proliferation, acid production and adhesion of Streptococcus mutans. Methods: Streptococcus mutans were cultured in MS medium containing different concentrations of plant extracts for 24 h, then centrifuged and washed. The bacteria cells were suspended in a certain volume of distilled water, and the optical density was measured by spectrophotometer. The pH of the supernatant was measured using a pH meter after centrifugation. The effect of the extract on the adhesion of Streptococcus mutans was observed by slide adhesion method. Results: Each extract and its compound inhibited the proliferation, acid production and adhesion of Streptococcus mutans (P<0.05). When the concentration was 24 g/L, the effect of green tea on the proliferation of Streptococcus mutans was stronger than that of other extracts (P<0.01). When the concentration was 0.75 g /L, 1.5 g / L and 3 g / L, the inhibitory effect of the compoundon Streptococcus mutans adhesion was stronger than that of other extracts (P<0.05) . Conclusion: All the three plant extracts have a certain anti-caries ability, and showed significant synergistic antibacterial activity. References | Related Articles | Metrics

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Expression and Clinical Significance of Rab38 in Oral Squamous Cell Carcinoma ZHENG Zheng, HE Ying-yang, XIE Di-ya, CHEN Yan, WANG Ze-zheng, HAO Feng-yao, WANG Zhi-yong 2017, 33(9): 946-949.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.009 Abstract ( 253 )   PDF (2334KB) ( 158 )   Objective: To investigate the expression of Rab38 in oral squamous cell carcinoma and its clinical significance. Methods: Rab38 mRNA expression was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in 60 matched OSCC and adjacent non-tumorous tissues. Expression of Rab38 was evaluated by immunohistochemistry in 149 cases of OSCC and matched adjacent non-tumorous tissues. Relationships between protein expression level and clinicopathological parameters were evaluated. Results: Rab38 was over-expressed in OSCC tissues. Clinical analysis indicated that high expression of Rab38 was obviously associated with TNM stage and lymph node metastasis. OSCC patients with high levels of Rab38 expression had shorter overall survival (OS). Multivariate cox regression analysis demonstrated that Rab38 expression remained an independent prognosis. Conclusion: Overexpression of Rab38 is closely related to poor prognosis. Rab38 may contribute to the development of OSCC, and may be used as a biomarker for evaluating outcomes. References | Related Articles | Metrics

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Effect of Silencing Tiam1 Gene by Small RNA Interference on Biological Characteristics of Tongue Squamous Cell Carcinoma Cells CHEN Hu-jie, DAI De-hua, DAI Jing, WEI Wei, XIN Hai-tao 2017, 33(9): 950-953.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.010 Abstract ( 212 )   PDF (2097KB) ( 206 )   Objective: To investigate the effect of silencing Tiam1 gene by small RNA interference (siRNA) on the biological characteristics of tongue squamous cell carcinoma cells. Methods: The human tongue cancer cells of SCC-4 were cultured. All cells were randomly divided into siRNA-Tiam1 group, siRNA-control sequence group, and blank control group. The expressions of Tiam1 gene and protein in different transfection groups were detected by real-time fluorescence quantitative PCR and Western blot, respectively. The cell proliferations in different transfection groups were detected by MTT assay. The cell apoptosis in different transfection groups were detected by flow cytometry. The cell migration and invasion in different transfection groups were detected by Transwell assay. Results: The relative expression levels of Tiam1 gene and protein in the siRNA-Tiam1 group were lower than those in the siRNA-control sequence group and blank control group (P<0.05). MTT assay showed that, compared with siRNA-control sequence group and blank control group, the absorbance A values at 24h, 48h, 72h and 96h in the siRNA-Tiam1 group decreased (P<0.05). Flow cytometry showed that the apoptosis rate in the siRNA-Tiam1 group was significantly higher than that in the siRNA-control sequence group and blank control group (P<0.05). The number of migrating cells and invasive cells in the siRNA-Tiam1 group was lower than that in the siRNA-control sequence group and blank control group (P<0.05). Conclusion: Specific silencing Tiam1 gene could effectively inhibit the proliferation of tongue squamous cell carcinoma cells, accelerate cell apoptosis, and inhibit cell migration and invasion ability. References | Related Articles | Metrics

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Research on Concentrated Growth Factor in Bone Regeneration LUO Ting-yuan, ZHANG Sen-lin 2017, 33(9): 954-957.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.011 Abstract ( 278 )   PDF (3706KB) ( 363 )   Objective: To evaluate local bone formation following surgical implantation of coralline hydroxyapatite (CHA) in combination with concentrated growth factor (CGF) using a rabbit calvarial defect model. Methods: Twenty-four rabbits were included in this randomized study. A circular defect in a diameter of 10mm was prepared on the parietal bone. The first group was filled with CHA/CGF and CHA. The second group was filled with CHA/CGF and void. The third group was filled with CGF and void. The bone formation was analyzed with microscopic computed tomography (micro-CT) and histological examination after 6 and 12 weeks. Results: With micro-CT analysis, bone volume and bone mineral density in the CHA/CGF group were the highest, followed by CGF group, and the blank control group was the lowest (P<0.05). Histological examination showed that new bone was distributed throughout the CHA scaffold in the specimens of CGF/CHA group after 6 weeks, and new bone appeared in the periphery region of the defects in the CGF group. The bone defects of CGF/CHA group were repaired fully with bone after 12 weeks, while those of the CGF group were repaired partly with bone. Conclusion: Combination of CHA and CGF could enhance the bone healing considerably. References | Related Articles | Metrics

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Effects of Inhibition of XBP1 Gene Expression on Biological Characteristics of Oral Squamous Cell Carcinoma by RNA Interference ZHANG Rui, YANG Yan-qiu, ZHONG Shi-yu, CHU Ya-nan 2017, 33(9): 958-961.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.012 Abstract ( 209 )   PDF (1777KB) ( 222 )   Objective: To detect the expression of X box binding protein 1 (XBP1) in human oral squamous cell carcinoma (OSCC) and to investigate the effect of inhibition the expression of XBP1 on proliferation and apoptosis of Tca8113. Methods: The expressions of XBP1 gene in the OSCC and paracancerous tissue were detected by RT-PC. NC-siRNA and XBP1-siRNA were transfected into Tca8113 cells, and the cells without any treatment were set the control group. The cell proliferation and apoptosis were detected after XBP1 gene transfection for 48h by CCK8 test and flow cytometry. The cleaved caspase3, β-catenin, and Cyclin D1 protein expression were detected by Western blot. Results: The mRNA expression of XBP1 gene in OSCC was significantly higher than that in paracancerous tissues (P<0.01). The expression of XBP1 protein and the cell viability were significantly decreased. Cell apoptosis were promoted. The protein expression of cleaved Caspase3 was increased, but the expressions of β-catenin and Cyclin D1 were decreased after XBP1-siRNA transfection (P<0.01). Conclusion: Silencing XBP1 gene expression by RNA interference can inhibit the proliferation of Tca8113 and induce apoptosis by inhibiting the Wnt/ β-catenin signaling pathway. References | Related Articles | Metrics

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Effect of Co-infection with Fusobacteriumnucleatum on Production of Reactive Oxygen Species from Polymorphonuclear Cells Stimulated by Porphyromonas Gingivalis or Aggregatibacter Actinomycetemcomitans LI Yan, YANG Chun-yu, LIU Zong-xia, ZHANG Yan-biao, LIANG Guang-zhi 2017, 33(9): 962-965.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.013 Abstract ( 286 )   PDF (4503KB) ( 238 )   Objective: To investigate the production of reactive oxygen species (ROS) from polymorphonuclear cells (PMNs) stimulated by porphyromonas gingivalis (P. gingivalis, Pg) or aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans, Aa) when co-infected with fusobacterium nucleatum (F. nucleatum, Fn) . Methods: Venous blood of healthy volunteers was obtained with heparin anticoagulation, the PMNs isolated with Percoll was mixed separated with 2’,7’-Dichlorodihydrofluorescindiacetate (DCFH-DA) and then incubated for 15 min in water bath at 37℃. Bacteria of group A and B (groups: A1, Pg; A2, Aa; A3, Fn;B1, Pg + Fn; B2, Aa + Fn; B3, Pg + Aa; B4, Pg + Aa + Fn; B5, Aa + Pg + Fn)were added into the tube for sufficient mixing, and then was incubated (37℃) for 1h and 4h. The amount of ROS produced by PMNs was measured by flow cytometry. Results: After the reaction of periodontopathic bacteria and isolated PMNs at 37℃ for 1h and 4h, the ROS production amounts were respectively A1-16.52; A2-18.74; A3-19.59; B1-17.60; B2-20.09. Among them, B1>A1, and B2>A2 (P<0.05). At the time of 4h, the amounts of ROS production were respectively A1-176.05; A2-203.19; A3-230.36; B1-259.08; B2-274.89. Among them, B1>A1 and B2>A2 (P<0.05). Conclusion: These results suggested that the production of ROS from PMNs by Pg and Aa can be enhanced when co-infected with Fn. References | Related Articles | Metrics

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Free Superficial Circumflex Iliac Artery Perforator Flap for Reconstruction of Soft Tissue Defects Secondary to Resection of Buccal Carcinoma RUAN Min, MA Chun-yue, ZHOU Hui-hong, TAO Xiao-feng, ZHANG Yi-xin, HE Yue 2017, 33(9): 966-969.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.014 Abstract ( 210 )   PDF (2803KB) ( 310 )   Objective: To investigate the therapeutic effect of free superficial circumflex iliac artery perforator (SCIP) flap for reconstruction of soft tissue defects secondary to resection of buccal carcinoma. Methods: From January 2014 to June 2014, six patients with buccal carcinoma received radical resection and immediate reconstructed with SCIP flap. CTA and color Doppler sonography were routinely performed before the surgery. Results: The flap sizes ranged from 30 to 63 cm2. The mean diameter of the superficial circumflex iliac arteries was 0.6 mm and of the veins was 1.1 mm. The mean arterial pedicle length was 6.9 cm, and the venous pedicle length was 7.3 cm. The mean reduction of mouth opening was 0.4 cm. No flap failure was found, and a satisfactory aesthetic outcome was obtained in all cases. Conclusion: The SCIP flap is a reliable, thin and pliable flap with long vascular pedicles 基金项目 国家自然科学基金(编号:81772870;81271112;81472517) 上海市浦江人才计划(编号:15PJD024) 上海市人才发展资助项目(编号:201312) 上海交通大学晨星学者"A"类及"B"类计划 作者简介 阮敏(1978~ ),男,安徽人,副主任医师,博士,主要从事口腔颌面部肿瘤的基础与临床研究。 *通讯作者 何悦,E-mail:doctorruanmin@hotmail.com and hidden donor site, and can achieve satisfactory postoperative buccal contour and mouth opening, making itself a good option for buccal defect reconstruction. References | Related Articles | Metrics

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Clinical Short-term Effects of Nd:YAG Laser and CO2 Laser on Minor Aphthous Ulcer LI Juan, LIU Zong-xiang, DUAN Ning, SUN Peng, WANG Wen-mei 2017, 33(9): 970-973.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.015 Abstract ( 278 )   PDF (802KB) ( 233 )   Objective: To compare the clinical short-term effects of Nd:YAG laser and CO2 laser in the treatment of minor aphthous ulcer (MiRAU). Methods: Sixty-eight cases of clinically diagnosed minor aphthous ulcer (MiRAU) patients were randomly divided into 2 groups, namely, the Nd:YAG laser treatment group (Nd:YAG laser group) and CO2 laser treatment group (CO2 laser group) with 34 cases in each group.The treatment period was 3-5 days. By the method of VAS and DP classification, the short-term curative effect of two groups was evaluated and compared. Results: In the first three days after laser treatment, the pain reduction index value of two groups was recorded, respectively. Index value of the Nd: YAG laser was: (3.27±0.24), (4.23±0.26) and (5.60±0.27), which was significantly higher than that of the CO2 laser group with the index value of (2.56±0.27), (3.40±0.30) and (4.20±0.28) (P<0.05). After the laser treatment,Ulcer healing time was(4.07±0.82)days in Nd:YAG laser group, which was significantly shortened than the(5.12±0.26)days in CO2 laser group (P<0.05). The total effective rate was 100.0% in Nd:YAG laser group and 90.0% in CO2 laser group. Nd: YAG laser group of patients showed no obvious reaction. And CO2 laser group has 6 cases of nervous pain, and 7 cases sensitive to smell. Conclusion: Significant effects on the treatment of MiRAU was observed between Nd:YAG laser and CO2 laser group. By comparison, Nd:YAG laser has more advantages in shortening the healing time and relieving pain. References | Related Articles | Metrics

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Clinical Study on Relationship between Risk of Coronary Heart Disease and Number of Tooth Loss in In-patients of Department of Cardiology ZHOU Tuan-tuan, WANG Chong, ZHANG Li-zhen, WANG Bing-na, LIU xin, XU Cai-xia, REN Xiu-yun 2017, 33(9): 974-977.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.016 Abstract ( 199 )   PDF (806KB) ( 227 )   Objective: To explore the correlation between the risk of coronary heart disease (CHD) and the number of tooth loss. Methods: Totally 506 in-patients result from chest pain were involved in Department of Cardiology of Cardiovascular Disease Hospital of Shanxi Province from May 2016 to November 2016. Basic information, oral health status, and general condition were collected using a standardized questionnaire. Based on the inclusion criteria, finally 328 cases were included in the research. According to the results of coronary angiography, three groups were divided:the non CHD group (degree of major vascular lumen diameter stenosis<50%), the single lesion group, and double or more lesions group. Results: The single factor analysis showed that age, gender, smoking, gingival index, and the number of tooth loss, were statistically significant (P<0.05). The ordinal logistic regression analysis showed that the number of tooth loss was a major risk factor for CHD incidence. Compared with no tooth loss, when the number of tooth loss was between 1-5, the risk of CHD increased by 83% (OR=1.825, 95% CI=1.074-3.1). When the number of tooth loss exceeded 5, the risk of CHD increased 1.65-fold (OR=2.65, 95% CI=1.471-4.956). Conclusion: In this in-patient, the number of tooth loss may be an independent risk indicator of increasing the incidence of CHD, and with the increasing of the number of tooth loss, the risk increasing; The risk inreased with age; And the risk of men was gredter than women. References | Related Articles | Metrics

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Influences of Eysenck Personality Factors on Dental Anxiety and Pain of Jaw Cyst Patients Treated by Cyst Plug after Fenestration and Decompression ZHENG Li, CHEN Ming-sheng, WANG Lu, GU Qiu-hua 2017, 33(9): 978-981.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.017 Abstract ( 211 )   PDF (2045KB) ( 208 )   Objective: To investigate the prevalence of dental anxiety, and study the influences of Eysenck personality factors on dental anxiety and pain of patients wearing cyst plug after fenestration and decompression. Methods: One hundred and twenty-six adult patients wearing cyst plug after fenestration and decompression were investigated with the EPQ, MDAS and VAS. The data was analyzed by SPSS 22.0 software. Results:The prevalence rate of DA in patients wearing cyst plug after fenestration and decompression was 60.32%. The score of MDAS was positively correlated to that of neuroticism (r=0.315) and psychoticism (r=0.241), and negatively correlated to extroversion (r=-0.322). Patients with DA had significantly higher scores of neuroticism and psychoticism and lower scores of extroversion than those without DA (P=0.000). The score of VAS was positively correlated to neuroticism (r=0.329) and psychoticism (r=0.386), and negatively correlated to extroversion (r=-0.237). Patients with DA had significantly higher scores of VAS than those without DA (P=0.009). The scores of MDAS was positively correlated to scores of VAS (r=0.234). Conclusion: Dental anxiety and pains of jaw cyst patients wearing cyst plug after fenestration and decompression are correlated to Eysenck personality. Patients who have higher score of neuroticism and psychoticism and lower scores of extroversion are more likely to suffer from dental anxiety and pain. References | Related Articles | Metrics

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Study on Facial Morphology of Uygur Youth using 3D Scanning in Xinjiang Area of Kashi ZHANG Xu. ZHAO Chun-ping, ZHANG Jia-yu, HE Hui-yu, GE Shu-peng, XIAO Feng, SHI Min 2017, 33(9): 982-986.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.018 Abstract ( 232 )   PDF (2626KB) ( 292 )   Objective: To compare the differences between 2D and 3D measurements of facial morphology of Uygur youthful males and females by using 3D scanner scanning in Xinjiang area of Kashi. Methods: Data from 341 healthy Uygur individuals were collected by 3DCaMega scanner (male 116 and female 225). Geomagic studio software was used to reconstruct 3D morphology, mark landmarks, set 3D coordinate system, confirm the reproducibility of the measurement, and measure 2D and 3D distances between landmarks of each sample. Results: All 9 measurements were confirmed perfect reproducibility (P>0.05). Comparison of facial distance parameters between Uygur males and females. There were significant differences between 2D and 3D distances for Len-Ren, Lex-Rex, N'-Sn and Sn-Mes measurements. No difference was found for Tr-N' and Lal-Ral measurements in 2D distances (P>0.05), but significant diffierence existed in 3D distances (P<0.05). Conclusion: 3D facial measurement is an accurate and reliable anthropometric method to reflect the differences of complex structures of human face. References | Related Articles | Metrics

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Clinical Study of Opalescence Whitening and ICON Penetrating Resin Technology in Bleaching for Dental Fluorosis MENG Qing-fei, ZHANG Jia-di, MENG Jian 2017, 33(9): 987-990.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.019 Abstract ( 489 )   PDF (2400KB) ( 1783 )   Objective: To evaluate the clinical effect of Opalescence whitening and ICON penetrating resin technology in bleaching for dental fluorosis. Methods: Twenty-one patients with dental fluorosis treated from October 2015 to October 2016 were given the treatment of Opalustre microabrasion, Opalescence whitening and ICON penetrating resin technology. Effect of bleaching was evaluated by Vita shade guide matching and teeth sensitivity was analyzed concurrently. Results: The shades for all patients were increased more than 5 degrees after teeth bleaching treatment and the total effective rate was 100%. The incidence rate of teeth sensitivity was 100% after Opalescence whitening, but faded away with one week. Only one patient had teeth sensitivity after ICON resin penetrating method. Conclusion: Opalustre microabrasion, Opalescence whitening, and ICON penetrating resin technology are effective, safe and minimally invasive for dental fluorosis. References | Related Articles | Metrics

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Branch Canal Anatomy of Maxillary First Premolars of Chinese People by Micro-CT BAI Yu-hao, WANG Fang-fang, LI Miao, WANG Ying, TANG Lu 2017, 33(9): 991-994.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.020 Abstract ( 274 )   PDF (2640KB) ( 299 )   Objective: To investigate the branch canal anatomy of the maxillary first premolars of Chinese people with micro-CT. Methods:Sixty-three maxillary first premolars were scanned by micro-CT and root canals were visualized by three-dimentional reconstruction. Branch canals and apical delta were recorded. The incidence, distribution and exit opening direction of branch canals were analyzed. Results: Branch canals of 50 teeth were recorded. The incidence of the branch canals was 79.37%. A total of 124 branch canals were recorded, and 95.16% of branch canals were located at the apical third of the root. The direction of the apical foramen of 33.06% branch canals were found at mesial, and the direction of the apical foramen of 23.39% branch canals was at distal side of the root. Incidence of apical delta was 7.94%. Conclusion: The root canal system of maxillary first premolars was complicated, with a high incidence of branch canals. It is important to know the branch canal morphology so as to increase the success rate of root canal treatment. References | Related Articles | Metrics

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Clinical Effects of Cantilever Implant Bridge in Lower Anterior Teeth WANG Zheng-hua, WANg Yuan, MA Chun-li, Baihetiyaer·Mahesuti, WANG Yuan-yuan, WANG Zhuo 2017, 33(9): 995-998.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.021 Abstract ( 269 )   PDF (791KB) ( 384 )   Objective: To analyze the clinical effects of cantilever implant bridge for lower anterior missing teeth with insufficient space. Methods: There were 30 patients who treated with cantilever and normal fixed implant bridge. The implant mobility before making models was collected and recorded. The PD, CAL, and GCF data were also recorded 0, 3, 6 and 12 months after restoration. The changes were analysed. Results: The indexes were not statistically significant within and between groups. Conclusion: Cantilever implant bridge with reasonable design showed a stable clinical effect, which could reduce the damage and the patient’s economic burden. References | Related Articles | Metrics

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Impact of Cusp Inclinations on Dental Fractures in Cracked Tooth Syndrome Model and Relevant Risk Evaluation XIE Ni-na, WU Cui, WANG Peng-lai, WANG Wen, LIU Zong-xiang 2017, 33(9): 999-1003.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.022 Abstract ( 264 )   PDF (2016KB) ( 339 )   Objective: To explore the impact of cusp inclinations on dental fractures in cracked tooth syndrome model and formulate corresponding risk scale. Methods: Forty maxillary premolars were randomized into four groups. For cracked tooth models, buccal and palatal cusp inclinations were different, with Group IV as blank control. All groups underwent compression loading test and fracture levels were recorded for statistical analysis. Results: The fracture modes included a majority of crown root fractures and a minority of crown fractures in Groups I and II, exclusive crown root fractures in Group III, and exclusive crown fractures in Group IV. Overall, palatal fractures were predominant versus buccal fractures, with exclusive palatal fractures in Group IV, and oblique fractures were overwhelming versus the scanty vertical fractures. Fracture risk classification: grade III was prevalent in Groups I and II, grade IV in Group III, and grades I and II in Group IV only. The fracture risk scores in Groups III and IV had significant statistical differences versus Groups I and II (P<0.05), with insignificant differences between Groups I and II. Conclusion: Cracked teeth are more vulnerable to complex fractures, with increment of cusp inclinations contributable to complex fracture modes, involving deep fracture and high risk scores. References | Related Articles | Metrics

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Repair of Difficult Complete Denture Cases for the Elderly LIU Guo-cun 2017, 33(9): 1004-1006.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.023 Abstract ( 239 )   PDF (773KB) ( 267 )   Objective: To observe the clinical effect of complete denture repair in the elderly patients with the old denture information. Methods: One hundred and thirty-two edentulous patients wearing old denture for more than 10 years were included and divided into two groups according to the age of the old denture. Experimental group: 66 cases were treated with polyether rubber according to the old denture; control group: making new dentures according to the conventional method. Results: Compared with the conventional method, the patients with full dentures made by the old denture were more adaptable and satisfactory. There were statistically significant differences in the masticatory efficiency and comfort satisfaction between two groups three days after wearing the new denture (P<0.05). Conclusion: It is necessary to make full use of old denture information to fabricate new denture to improve the satisfaction for elderly patients. References | Related Articles | Metrics

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Application of Digital Surgical Guide in Immediate Implantation BAO Fan, LIU Juan, GUO Hui, LIU Ying-ting 2017, 33(9): 1007-1011.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.024 Abstract ( 257 )   PDF (5785KB) ( 380 )   Objective: To evaluate the application effect of digital surgical guide in immediate implantation. Methods: Twenty-two patients were included in this study from January 2015 to January 2017. Thirty-two implants were placed precisely with the assist of surgical guide plates. Imagining materials was collected to measure the deviation between the virtual and actual implant position. The crest bone level,M-T bone width, and the soft tissue index at the immediate postoperative or after one year were measured and analyzed. Results: The implant angel deviation was (3.25±1.6) degree, the position deviation was (0.54±0.38)mm, and the length deviation was (0.51±0.38)mm. The difference showed statistical significance. After six months, the vertical bone resorption was (1.3±0.6)mm and M-T bone resorption at alveolar crest was (0.8±0.3)mm. The index of soft tissue mBI was (0.91±0.45), and the PD was (2.05±0.3)mm. After one year follow-up,the mBI was (1.18±0.72), and the PD was (2.10±0.2)mm. The difference of mBi showed statistical significance. Conclusion: The application of surgical guide plates is beneficial to immediate implantation. References | Related Articles | Metrics

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Advancement on Correction of MAPK Signal Pathway and Chronic Periodontitis ZHAO Na, FANG Hui, TANG Ya-ping, LIU Qi. School of Stomatology, Zunyi Medical College 2017, 33(9): 1012-1015.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.025 Abstract ( 345 )   PDF (820KB) ( 546 )   Till now, the exact pathogenesis of chronic periodontitis still needs exploration. Recently, the MAPK signal transduction pathway has became a research hot spot in chronic periodontitis. This review focuses on the regulatory effects of MAPK signals in the inflammatory reactions and immune response in periodontium, and the destroy and regeneration in alveolar of chronic periodontitis. References | Related Articles | Metrics

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Research Progress of MicroRNAs in Field of Periodontics XUE Nan, QI Lin, LIU Huan-ye, ZHANG Yang 2017, 33(9): 1016-1018.  DOI: 10.13701/j.cnki.kqyxyj.2017.09.026 Abstract ( 288 )   PDF (799KB) ( 258 )   MicroRNAs (miRNAs) are endogenously expressed small noncoding RNAs that regulate gene expression. It plays a critical role in cell proliferation, cell differentiation, and organism development. Current researches on miRNAs mainly focus on its antitumor effect while its role in periodontics lacks attention. This review is focusing on the differential expression of miRNAs in periodontitis, osteoblast, and osteoclast differentiation, and discussing their possible regulation mechanism on the basis of the latest progress on researches of miRNAs in periodontitis. References | Related Articles | Metrics