Research on Concentrated Growth Factor in Bone Regeneration

doi:10.13701/j.cnki.kqyxyj.2017.09.011

Abstract

Abstract: Objective: To evaluate local bone formation following surgical implantation of coralline hydroxyapatite (CHA) in combination with concentrated growth factor (CGF) using a rabbit calvarial defect model. Methods: Twenty-four rabbits were included in this randomized study. A circular defect in a diameter of 10mm was prepared on the parietal bone. The first group was filled with CHA/CGF and CHA. The second group was filled with CHA/CGF and void. The third group was filled with CGF and void. The bone formation was analyzed with microscopic computed tomography (micro-CT) and histological examination after 6 and 12 weeks. Results: With micro-CT analysis, bone volume and bone mineral density in the CHA/CGF group were the highest, followed by CGF group, and the blank control group was the lowest (P<0.05). Histological examination showed that new bone was distributed throughout the CHA scaffold in the specimens of CGF/CHA group after 6 weeks, and new bone appeared in the periphery region of the defects in the CGF group. The bone defects of CGF/CHA group were repaired fully with bone after 12 weeks, while those of the CGF group were repaired partly with bone. Conclusion: Combination of CHA and CGF could enhance the bone healing considerably.

Key words: Coralline hydroxyapatite, Concentrated growth factors, Bone defect, Repairment

CLC Number:

R782.1

LUO Ting-yuan, ZHANG Sen-lin. Research on Concentrated Growth Factor in Bone Regeneration[J]. Journal of Oral Science Research, 2017, 33(9): 954-957.

References

[1] Chen Y, Cai Z, Zheng D, et al. Inlay osteotome sinus floor elevation with concentrated growth factor application and simultaneous short implant placement in severely atrophic maxilla [J]. Scientific Reports, 2016, 6∶27348
[2] Intini G. The use of platelet-rich plasma in bone reconstructions therapy [J]. Biomaterials, 2009,30∶4956-4966
[3] Schaaf H, Strechbein P, Lendeckel S, et al. Sinus lift augmentation using autogenous bone grafts and platelet-rich plasma: radiographic results [J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2008, 106(6)∶673-678
[4] Dohan DM, Choukroun J, Diss A, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate, part Ⅱ platelet-related biologic features [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endon, 2006,101∶e45-50
[5] Sezgin Y, Uraz A, Taner IL, et al. Effects of platelet-rich fibrin on healing of intra-bony defects treated with anorganic bovine bone mineral [J]. Brazilian Oral Research, 2017, 31(0)∶e15
[6] Sacco L, Lecture[C]. International Academy of Implant Prosthesis and Osteoconnection, 2006.12.4
[7] 于鑫玮,高承志.自体静脉血在不同离心条件下特定生长因子成分测定与分析性研究[J].中国医药导刊, 2016,18(9)∶865-868
[8] Kim JM, Lee JH, Park IS. New bone formation using fibrin rich block with concentrated growth factors in maxillary sinus augmentation [J]. J Korean Assoc Oral Maxillofac Surg, 2011, 37(2)∶278-286
[9] Tadic, A, Puskar T, Petronijevic B. Application of fibrin rich blocks with concentrated growth factors in pre-implant augmentation procedures [J]. Medicinski pregled, 2014, 67(5-6)∶177-180
[10] 黄娜,吴锋,李平,等.应用浓缩生长因子行上颌窦内提升后种植体周围骨变化[J].中国组织工程研究,2015,19(47)∶7575-7582
[11] Simonpieri A, Choukroun J, Del CM, et al. Simultaneous sinus-lift and implantation using microthreaded implants and leukocyte- and platelet-rich fibrin as sole grafting material: a six-year experience [J]. Implant Dentistry, 2011, 20(1)∶2-12
[12] 杨立明,陈淑萍,李恩洪.浓缩生长因子在即刻种植修复应用中的牙龈美学研究[J].中国美容医学,2015,24(1)∶63-65
[13] Luo Z, Zhang Q, Zhang Z, et al. Performance of coralline hydroxyapatite in sinus floor augmentation: a retrospective study [J]. Clin Oral Invest, 2013, 17(9)∶2003-2010
[14] 杨烁,徐淑兰,肖希娟,等.可降解珊瑚羟基磷灰石与脱矿无机牛骨在上颌窦提升术后改建的临床对比研究[J].实用口腔医学杂志,2013,29(3)∶334-338

[1]	XU Laijun, LI Jiyao. Research Progress on Gellan Gum Composite in the Field of Bone Defect [J]. Journal of Oral Science Research, 2020, 36(6): 512-515.
[2]	LI Ailing, ZHOU Hong. Clinical Application of “Sandwich” Osteotomy for Implant Restoration at Deficient Anterior Alveolar Ridges [J]. Journal of Oral Science Research, 2020, 36(3): 293-297.
[3]	XUE Guoping, LIU Qingmei. An Update of Low-level Laser Therapy Applied for Bone Regeneration of Jaws [J]. Journal of Oral Science Research, 2019, 35(9): 830-832.
[4]	YU Wenfeng, ZHAO Shijun, LV Minmin, CUI Fenglin, KANG Meizhen, ZHANG Zhiyuan. Clinical Application of Concentrated Growth Factors in Guided Bone Regeneration of Maxillary Anterior Region [J]. Journal of Oral Science Research, 2019, 35(7): 676-680.
[5]	LI Ji-yin, HE Ping. Clinical Application Progress of Autogenous Tooth Bone Graft Material in Repairing Alveolar Bone Defect. [J]. Journal of Oral Science Research, 2019, 35(3): 212-214.
[6]	ZHENG Jun-yuan, HE Li, HU Tu-qiang, WANG Mei-jun. Half-year Effect of Er-YAG in the Treatment of Periodontal Angular Bone Defect of Premolars in Elderly Patients: A Case Report [J]. Journal of Oral Science Research, 2019, 35(3): 307-308.
[7]	WANG Chao, XIA Rong, LIU Rui, XIAO Nan-nan. Experimental Study on Reconstruction of Alveolar Ridge Defects with Personalized Titanium Mesh Based on 3D Printing Technology [J]. Journal of Oral Science Research, 2019, 35(1): 80-83.
[8]	QI Man-lin, ZHOU Yan-min.. Properties of Polyetherketoneketone and Its Application in Stomatology. [J]. Journal of Oral Science Research, 2018, 34(9): 939-942.
[9]	ZHOU Jing, SUN Xiao-lin, QI Man-lin, DING Xin-xin, LI Xue, ZHOU Yan-min. Application of Platelet Rich-Fibrin in Posterior Immediate Implantations: A Case Report [J]. Journal of Oral Science Research, 2018, 34(1): 92-93.
[10]	ZHONG Zhi-hua, TANG Yi, SHI Bin.. Application of Bicon Implant and Maxillary Sinus Inner Lift Technology for Upper Posterior Missed Teeth. [J]. Journal of Oral Science Research, 2017, 33(7): 787-789.
[11]	ZHU Zhong-yan, ZHANG Lu, LIU Min. Effects of Autologous Concentrated Growth Factors Extract on MC3T3-E1 Cells [J]. Journal of Oral Science Research, 2017, 33(11): 1147-1150.
[12]	JIN Xia-ying, ZHONG Wei-jian, MA Guo-wu. Application of Autologous Tooth as Bone Graft Material in Upper Anterior Teeth Implant Restoration: A Case Report [J]. Journal of Oral Science Research, 2017, 33(11): 1228-1229.
[13]	XIAO Qiong, DONG Lu, YANG Qin-qiu,SUN Yong, CHEN Hong-liang, ZHAO Feng, LU Shuai. Microstructure Studies on Platelet-rich Fibrin Repairing Bone Defects in the Beagle Dog [J]. Journal of Oral Science Research, 2016, 32(8): 794-799.
[14]	DANG Hai-xia, HUO Xin-ru, ZHANG Wen-yuan, ZHANG Lin, WANG Fu.. 14, 15- Epoxyeicosatrienoic Acid Promotes Bone Regeneration of Mouse Calvarial Defect. [J]. Journal of Oral Science Research, 2016, 32(7): 692-695.
[15]	LIU Wei, CHEN Jie, HU Kai-jin, ZHANG Lin-lin, DING Yu-xiang. Sustain-released Gelatin Microspheres Loaded with CGRP/SP for Repairing Bone Defect of Osteoporosis Rabbits. [J]. Journal of Oral Science Research, 2016, 32(3): 228-233.