Abstract: Objective: To observe the microstructure of new bone formed from PRF induction and evaluate the features and advantages of PRF on bone repair by the femoral condyle defect model inbeagle dogs, providingnew experimental basis forosteanagenesis and mandibular reconstruction. Methods: The PRF membrane was prepared by means of Choukroun. PRF, Bio-Oss bone substitute materials and autologous cancellous bone were randomly filled into bone defects in the femoral condyle of dog. At 12 weeks and 24 weeks,the dogswere sacrificedand the bone was retrieved. Micro-CT was used to detect and analyze geometry and structure information of new bone. Results: New bone formedfrom PRF hadplate-shape and thicker trabecular structure with good continuity and maturity, which was highly close to that inthe autogenous bone group and better than that in the Bio-Oss group. During the observation period, the average values were closeto natural bone. In bone regeneration process, PRF osteoinduction showed concentric features. Conclusion: PRF could induce the formation of new bone, microstructures of which were similar to physiological conditions and showed central regeneration, thus showing a prospect for clinical application.

Key words: Platelet-rich fibrin, Bone defects, Osteoinduction, Micro-CT