Abstract: Objective: To study the effectsof exosomes secreted byoral squamous cell carcinoma cells (OSC-4) on their biological behaviors. Methods: Exosomesderived from OSC-4 cells were separated from the medium. The effects of exosomes on the OSC-4 cell proliferation, migration and invasion were detected by MTT, wound healing, andtranswellassays, respectively. Results: Gold-labeling CD9 was detected in the isolated exosomes. MTT assay showed that the OSC-4 cells proliferation ability was increased after treated with exosomes(P<0.05). Wound healing and transwell assays results showed that the migration and invasion ability of OSC-4 cells were also increased after treated byexosomes (P<0.05). Conclusion: Exosomes secreted byoral squamous cell carcinomacells couldpromote their proliferation, migration, and invasion.

Key words: Oral squamous cell carcinoma, Exosome, Biological behavior