Osteogenesis of Human Dental Follicle Stem Cells in Vitro

doi:10.13701/j.cnki.kqyxyj.2016.08.004

Abstract

Abstract: Objective: To observe the osteogenesis of human dental follicle stem cells(DFSC) in osteogenic medium in vitro. Methods: DFSCs were primarily cultured by tissue and digestion method. Cells were passaged and identified by stem cell surface marker expression using immunohistochemistry and flow cytometry. After osteogenesis induction,osteogenesis ability of the cells was determined with alkaline phosphate (ALP) and Alizarin Red S staining, real time quantitative PCR and western blot. Results: DFSCs showed strong reproductive activity. And the immunohistochemistry staining showed that vimentin expression in DFSCs was positive, while cytokeratin 14 was negative. High expression of mesenchymal stem cell markers CD44, CD90, CD105 and CD146 was detected in DFSCs. Under osteogenesis induction, DFSCs could express osteogenesis or cementoblast cell markers ALP, OCN, BMP2 and RUNX2 in vitro(P＜0.05). Conclusion: DFSC might be a novel candidate for the treatment of periodontal defects in periodontal tissue engineering.

Key words: Dental follicle stem cells, Osteogenic differentiation, Periodontal tissue engineering

CLC Number:

R781.4

WANG Chen,XU Yan, YANG Yang,MENG Ming-li,WANG Jing,ZHOU Yong-min,WANG Xiao-jing. Osteogenesis of Human Dental Follicle Stem Cells in Vitro[J]. Journal of Oral Science Research, 2016, 32(8): 789-793.

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