Sustain-released Gelatin Microspheres Loaded with CGRP/SP for Repairing Bone Defect of Osteoporosis Rabbits.

doi:10.13701/j.cnki.kqyxyj.2016.03.005

Abstract

Abstract: Objective: To evaluate the impact of sustain-released gelatin microspheres loaded with CGRP/SP on the osteogenesis of the bone defect in osteoporosis rabbits. Methods: New Zealand white rabbit model was established by ovariectomy. The DEXA was used to detect the rabbits lumbar vertebrae BMD. Sustain-released gelatin microspheres loaded with 10^-6mol/L, 10^-8mol/L and 10^-10mol/L of CGRP/SP were prepared via emulsion cross-linking method. Twenty-four rabbit models were randomly assigned to receive sustain-released gelatin microspheres loaded with different concentrations of CGRP/SP into the bone defects. The release in vitro within 1 month was measured by ELISA method. The rabbits were killed 3 months after the implanting to evaluate the impact of osteogenesis. Results: Gelatin microspheres loaded with CGRP/SP had better effect than the blank control group. The effect reaches the best when the concentration of SP was 10^-6mol/L and CGRP was 10^-8mol/L. And the effect reached the best when the concentration of SP and CGRP was 10^-6mol/L. Conclusion: Gelatin microspheres loaded with CGRP/SP could improve the new bone formation significantly in contrast to the blank control group. Higher concentration was better.

Key words: Calcitonin gene related peptide , Substance P , Gelatin microspheres, Bone osteoporosis , Bone defect

CLC Number:

R782.2

LIU Wei, CHEN Jie, HU Kai-jin, ZHANG Lin-lin, DING Yu-xiang. Sustain-released Gelatin Microspheres Loaded with CGRP/SP for Repairing Bone Defect of Osteoporosis Rabbits.[J]. Journal of Oral Science Research, 2016, 32(3): 228-233.

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