Effect of Canonical Wnt Signalling Pathway on the Osteogenic Differentiation and Proliferation of PDLCs.

doi:10.13701/j.cnki.kqyxyj.2016.03.004

Abstract

Abstract: Objective: To investigate the effects of canonical Wnt signalling pathway on the osteogenic osteogenic differentiation of human periodontal ligament cells (HPDLCs). Methods: HPDLCs were obtained through in vitro culture combined with enzyme digestion method and tissue pieces culture method. The existence of canonical Wnt signalling pathway was examined by RT-PCR and immunocytochemistry. Lithium chloride (LiCl) was applied during osteogenesis, and both Alizarin red staining and Alkaline phosphatase activity detection were used to investigate influence of Wnt/β-catenin signaling pathway on the osteogenic differentiation of PDLCs．CCK-8 assay was performed to study the effect of activated canonical Wnt signalling pathway on the proliferation of PDLCs. Results: The expression of canonical Wnt signaling pathway conponents were strongly expressed in PDLCs. Negative effects of canonical Wnt signaling pathway on osteoblast differentiation were proved by Alizarin red staining and Alkaline phosphatase activity detection (P<0.01). CCK-8 assay also showed the inhibition of canonical Wnt signaling pathway on the proliferation of PDLCs. Conclusion: Activation of canonical Wnt pathway may inhibit the osteogenic differentiation and proliferation of PDLCs.

Key words: Human periodontal ligament cells, Osteogenic differentiation , Canonical , Wnt signalling pathway

CLC Number:

R781.4

LIAO Hai-qing, CAO Zheng-guo. Effect of Canonical Wnt Signalling Pathway on the Osteogenic Differentiation and Proliferation of PDLCs.[J]. Journal of Oral Science Research, 2016, 32(3): 224-227.

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