Effect of New Type Multistage Aperture Bioactive Glasses Prepared by Pollen and P123 Dual Template on Proliferation and Differentiation of MG63 Cells

doi:10.13701/j.cnki.kqyxyj.2017.09.003

Abstract

Abstract: Objective: To investigate the effects of new type multistage aperture bioactive glasses which were prepared by pollen and P123 dual template on the proliferation and differentiation of MG63 cells. Methods: MG63 cells were cultured in the material leach liquor (0.2g/ml working concentration, DMEM with 10% fetal bovine serum) for 1, 3, and 5 days. MTT colorimetric method was used to detect the level of cell proliferation. Alkaline phosphatase kits was used to measure the ALP activity. Real-time PCR was used to analyze the mRNA expression of osteogenesis marker, i.e. RUNX-2, SP7, and COL-1. Results: The material promoted the proliferation of MG63 cells, enhanced the activity of ALP, up-regulated the mRNA expression level of markers gene RUNX-2, SP7, and COL-1. Conclusion: The new type multistage aperture bioactive glasses which were prepared by pollen and P123 dual template has low cytotoxicity and the potential to promote the osteogenetic differentiation.

Key words: Multistage aperture bioactive glasses, MG63 , Proliferation , Differentiation

CLC Number:

R339.2

WANG Yang,SHEN Yu-qin,LING Xin-lian,LIU Yu-yan,QIN Tian-tian,DIAO Meng-xue,SUN Xin-hua. Effect of New Type Multistage Aperture Bioactive Glasses Prepared by Pollen and P123 Dual Template on Proliferation and Differentiation of MG63 Cells[J]. Journal of Oral Science Research, 2017, 33(9): 920-923.

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