Abstract: Objective: To study the effects of salidroside (SAL) on proliferation, differentiation, mineralization, and the expression of VEGF depending on HIF-1α of MG-63 cells and related molecular mechanisms. Methods: To study the effects SAL on proliferation, differentiation, mineralization, the expression of VEGF depending on HIF-1α, and related molecular mechanisms, the cell vitality assay, Flow cytometry analysis, semi-quantitative reverse transcription polymerase chain reaction, ELISA and Western blot were used. Results: SAL promoted the proliferation and differentiation of MG-63 cells through regulating the distribution of cell cycle, the mRNA and protein levels and the expression of HIF-1α. SAL could also increase the VEGF production of downstream target genes of MG-63 cells. Conclusion: In vitro experiment study shows that SAL can promote proliferation, differentiation, mineralization, the production of HIF-1α and downstream target genes VEGF of MG-63 cells to promote the formation of new bones and vessels.

Key words: Salidroside (SAL) , Osteoblast, Hypoxia inducible factor-1α (HIF-1α) , Vascular endothelial , growth factor (VEGF)