Effects of DVDMS-PDT on Proliferation and Migration of Human Tongue Squamous Carcinoma Cell Line HSC-4

doi:10.13701/j.cnki.kqyxyj.2018.11.015

Abstract

Abstract: Objective: To study the proliferation and migration of oral tongue squamous cell carcinoma HSC-4 cells induced by DVDMS-PDT. Methods: To detect the cytotoxicity of DVDMS and the uptake of drugs by cells, the cells were divided into control group and DVDMS-PDT treatment group with different concentrations (0.1, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, and 8.0 mg/L, respectively). The laser energy density were 0.75, 1.50, 3.00, 6.00J/cm². The effects of different drug concentrations and light energy density on cell viability were analyzed by CCK-8. Scratch assay and Transwell experiment were used to observe the changes of cell migration ability. Results: DVDMS toxicity experiments showed that the single drug had less cytotoxicity, and combined with laser irradiation, it could significantly inhibit the proliferation of HSC-4 cells (P<0.05). Compared with the control group, the healing rate of scratch assay was decreased,and the number of transwell penetrated cells was decreased (P<0.05). DVDMS concentration at 2.0 mg/L, 6 hours incubation, and light energy density above 3.00J/cm² could effectively kill HSC-4 cells. Conclusion: DVDMS-PDT can effectively kill the tongue squamous cell carcinoma cell line and inhibit the migration of HSC-4 cells. Photosensitizer concentration, incubation time, and light energy density are important factors affecting the therapeutic effect.

Key words: Sinoporphyrin sodium, Photodynamic therapy, Tongue squamous cell carcinoma, Migration

HUANG Yu-si, ZHANG Lei, LIU Le-tian, MA Yao, LIU Xin. Effects of DVDMS-PDT on Proliferation and Migration of Human Tongue Squamous Carcinoma Cell Line HSC-4[J]. Journal of Oral Science Research, 2018, 34(11): 1208-1211.

References

[1] Jansson L, Kalkali H, Niazi FM. Mortality rate and oral health-a cohort study over 44 years in the county of Stockholm [J]. Acta Odontol Scand, 2018, 76(4)∶299-304
[2] Straten DV, Mashayekhi V, Bruijn HSD, et al. Oncologic photodynamic therapy: basic principles, current clinical status and future directions [J]. Cancers (Basel), 2017, 9(2). pii∶E19.
[3] 方起程. 癌症光动力治疗和新抗癌光敏剂华卟啉钠[J]. 中国新药杂志, 2014, 23(13)∶1540-1545
[4] Liu Y, Wang P, Liu Q, et al. Sinoporphyrin sodium triggered sono-photodynamic effects on breast cancer both in vitro and in vivo [J]. Ultrasonics Sonochemistry, 2016, 31∶437-448
[5] 胡建敏.新型光敏剂华卟啉钠介导的光动力疗法对小鼠乳腺癌抗肿瘤效应研究[D].陕西师范大学,2015
[6] Shi R, Li C, Jiang Z, et al. Preclinical study of antineoplastic sinoporphyrin sodium-PDT via in vitro and in vivo models [J]. Molecules, 2017, 22(1),pii∶E112
[7] Filonenko EV, Kaprin AD, Alekseev BYA, et al. 5-Aminolevulinic acid in intraoperative photodynamic therapy of bladder cancer (results of multicenter trial) [J]. Photodiagnosis Photodyn Ther, 2016, 16∶106-109
[8] 赵艳,张壁,陈琦,等.STAT3促进舌鳞癌细胞侵袭与转移的实验研究[J].口腔医学研究, 2015, 31(7)∶688-690
[9] Salimi Sartakhti J, Manshaei MH, Sadeghi M. MMP-TIMP interactions in cancer invasion: An evolutionary game-theoretical framework [J]. J Theor Biol, 2017, 412(18)∶17-26
[10] Wu L, Wang X, Liu Q, et al. Sinoporphyrin sodium mediated photodynamic therapy inhibits the migration associated with collapse of F-actin filaments cytoskeleton in MDA-MB-231 cells [J]. Photodiagnosis Photodyn Ther, 2016, 13∶58-65
[11] Rong P, Yang K, Srivastan A, et al. Photosensitizer loaded nano-graphene for multimodality imaging guided tumor photodynamic therapy[J]. Theranostics, 2014, 4(3)∶229-239

[1]	GUAN Mengying, HE Lina, PAN Shuang, LI Yanping, LIU Huimei, NIU Yumei. Effects of Canonical Wnt Signaling Pathway on Proliferation, Migration, and Odontogenic Differentiation of hDPSCs [J]. Journal of Oral Science Research, 2020, 36(9): 861-865.
[2]	PEI Xiyan, SUN Feng. Research Progress on Minimally Invasive Non-surgical Periodontal Therapy [J]. Journal of Oral Science Research, 2020, 36(7): 622-625.
[3]	LI Tianke, ZHANG Ying, ZHANG Suxin, CHEN Zhong, BAO Yang, ZHANG Guanhua. Identification of Hub Genes in Tongue Squamous Cell Carcinoma Based on Weighted Gene Co-expression Network Analysis [J]. Journal of Oral Science Research, 2020, 36(5): 423-427.
[4]	WANG Xueteng, NIE Minhai, ZUO Dongchuan, DENG Hao, ZENG Jin. Effect of TRPM4 Channel on Proliferation and Migration of Human Buccal Mucosal Fibroblasts [J]. Journal of Oral Science Research, 2020, 36(5): 490-495.
[5]	WEI Diyuan, YAN Zhimin. Research Progress and Application Potential of Photodynamic Therapy in Treating Oral Candidiasis [J]. Journal of Oral Science Research, 2020, 36(12): 1087-1090.
[6]	ZHOU Qun, QIU Jiaxuan. Plumbagin Induces Tongue Squamous Cell Carcinoma Cells Apoptosis via ROS-mediated JNK Signaling Pathway [J]. Journal of Oral Science Research, 2020, 36(10): 921-924.
[7]	XIE Long, SANG Lei, SONG Zhuoying, JIANG Yanjun. Diagnostic Values of Salivary MicroRNA-375 for OSCC and Its Effects on Biological Function of OSCC Cells [J]. Journal of Oral Science Research, 2019, 35(9): 883-888.
[8]	DENG Xuan, YANG Fang, TANG Xiqing. Effect of Down-regulation of Mucin-1 on Growth of Xenografts of Human Tongue Squamous Cell Carcinoma Tca-8113 in Nude Mice [J]. Journal of Oral Science Research, 2019, 35(8): 776-780.
[9]	MENG Wenxia, FENG Lu, LIU Shuguang, ZHOU Huixi, LI Zhiqiang. Effect of Oral Lichen Planus-associated Myofibroblasts on Migration and Tube-formation of Vascular Endothelial Cells [J]. Journal of Oral Science Research, 2019, 35(8): 806-809.
[10]	WANG Rui, LI Li-heng, XU Ai-wen, AN Feng. Lentivirus Mediated Down-regulation of Med19 Expression Enhances Sensitivity of Tongue Squamous Cell Carcinoma to Cisplatin [J]. Journal of Oral Science Research, 2019, 35(6): 559-562.
[11]	ZHOU Tong, ZHANG Tong-fei, ZHANG Ze-bing, QUAN Hai-ying. Influence of MSA on Proliferation, Migration, Apoptosis, and Cell Cycle of Oral Squamous Cell Carcinoma Tca8113 and KB Cells [J]. Journal of Oral Science Research, 2019, 35(6): 568-572.
[12]	ZHANG Xin-yu, WU Shuang-yan, WU Hong, XU Zhu-qing, WANG Yun-ying, XU Xiao-na, LI Xin, ZHENG Jian-jin. Effect of Human Umbilical Cord Mesenchymal Stem Cells on Invasion and Migration of Cal-27 Cells from Tongue Squamous Cell Carcinoma. [J]. Journal of Oral Science Research, 2019, 35(5): 443-447.
[13]	LI Yong-wei, QIU Xun-ding, GU Ming, DENG Yi, XIE Li-li. Effect of FMNL2 Gene on Invasion, Migration, and NF-κB Signal in Oral Squamous Cell Carcinoma. [J]. Journal of Oral Science Research, 2019, 35(2): 137-141.
[14]	SUN Yuhong, YANG Liu, WANG Shuo, XIA Lulu, GUO Li, CAO Zhengguo, HUA Xianming. Combination of Periodontal and Orthodontic Treatment for Severe Chronic Periodontitis Patient with Upper Migrated Incisor: A Case Report [J]. Journal of Oral Science Research, 2019, 35(11): 1100-1102.
[15]	WEI Wei, TANG Yi, DAI Jing, CHEN Hujie. Effects of MicroRNA-218-5p Targeting ABCG2 Gene on Proliferation, Migration, and Invasion of Tca-8113 Cells. [J]. Journal of Oral Science Research, 2019, 35(10): 984-988.