Effect of Plumbaginon on Invasion and Migration of Tongue Squamous Cell Carcinoma Cells and Its Mechanism

doi:10.13701/j.cnki.kqyxyj.2018.01.010

Abstract

Abstract: Objective: To investigate the relationship between Glucose transporter isoform 1 (GLUT1) and metastasis of tongue squamous cell carcinoma (TSCC) and the effect and mechanism of plumbagin (PLB) on the invasion and migration of TSCC cells. Methods: The relationship between metastasis of TSCC and GLUT1 or Matrix metalloprotein 2 (MMP2) was detected by immunohistochemical assay. The proliferation inhibition effect and the half inhibitory concentration (IC50) of plumbagin on TSCC cells were detected by MTT assay. The invasion ability and migration ability were detected by Transwell assay and wound healing test, respectively. And the expressions of p53, GLUT1, and MMP2 were evaluated by Western blot. Results: The high expression of GLUT1 and MMP2 was closely related to the metastasis of TSCC (P<0.05). MTT assay showed that the IC50 of PLB to TSCC cells for 24h was 6.78μmol/L. Transwell assay and wound healing test showed that PLB and NSC207895 and WZB117 significantly reduced the number of transmembrane cells and cell mobility. Western blot showed that the expression of p53 increased, while the expression of GLUT1 and MMP2 decreased after treatment of PLB (P<0.05). The expression of GLUT1 and MMP2 decreased (P<0.05) after treatment of NSC207895. And the expression of MMP2 decreased (P<0.05) after treatment of WZB117. However, the expression of p53 was not changed (P>0.05). Conclusion: The high expression of GLUT1 is closely related to the metastasis of TSCC. Plumbagin can inhibit the invasion and migration of TSCC through p53/GLUT1/MMP2 pathway.

Key words: Tongue squamous cell carcinoma, Invasion, Migration, Plumbagin, Glucose transporter isoform 1

CLC Number:

R739.8

ZHOU Xiong-ming, NA Si-jia, PAN Shu-ting, QIU Jia-xuan. Effect of Plumbaginon on Invasion and Migration of Tongue Squamous Cell Carcinoma Cells and Its Mechanism[J]. Journal of Oral Science Research, 2018, 34(1): 39-43.

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