Expression and Significance of Vitronectin in Blood Serum of Oral Squamous Cell Carcinoma

doi:10.13701/j.cnki.kqyxyj.2018.01.011

Abstract

Abstract: Objective: To observe the expression of blood serum vitronectin (VTN) in oral squamous cell carcinoma (OSCC), and to explore its clinical significance and the degree of differentiation, as well as the correlation between cervical lymph node metastasis. Methods: Western blot method was used to detect VTN expression in 26 OSCC patients without lymph node metastasis, including 18 OSCC patients with lymph node metastasis, 8 oral tissue atypical hyperplasia patients, 20 healthy people from Department of Oral and Maxillofacial Surgery, affiliated Stomatological Hospital of Harbin Medical Universit from 2016,08,01-2017,05,30. Results: VTN was highly expressed in the blood serum of healthy people (36849.10±1127.37) than that of OSCC patients without lymph node metastasis (22518.12±1301.25). VTN was also highly expressed in the blood serum of OSCC patients without lymph node than that of OSCC patients with lymph node metastasis (9459.28±629.58, P<0.001). VTN was 36725.50±1484.41 in the blood serum of patients with atypical hyperplasia of oral mucosa. The expression of VTN in OSCC patients with different differentiated degree and without lymph node metastasis was analyzed. The results showed that VTN was highly expressed in the blood serum of high differentiated OSCC patients (59009.13±145.75) than that of moderately differentiated OSCC patients (39468.82±1135.61). And its expression was also higher than that of poorly differentiated OSCC patients (22437.00±1286.38, P<0.001). Conclusion: VTN may be associated with the malignant transformation and mestastasis of OSCC, and expression of serum VTN may help OSCC diagnosis and disease progression assessment.

Key words: Vitronectin, Oral squamous cell carcinoma, Western blotting

CLC Number:

739.8

TIAN Bing, HU Teng-long, JING Guang-ping, YU Yang, ZHONG Ke-tao, WANG Hai-tao, YU Qing. Expression and Significance of Vitronectin in Blood Serum of Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2018, 34(1): 44-46.

References

[1] Mayne ST,Morse DE,Winn DM. Cancers of the oral cavity and pharynx [M]//Schottenfeld D,Fraumeni JF,editors.Cancer epid-emiology and prevention.3rd ed.New York:Oxford University Pr-ess,2006∶674-696
[2] Omura K. Current status of oral cancer treatment strategies:s-urgical treatments for oral squamous cell carcinoma [J]. Int J Clin Oncol,2014,19(3)∶423-430
[3] Brocklehurst PR,Baker SR,Speight PM. Oral cancer screening:what have we learnt and what is there still to achieve [J]. Future Oncol,2010,6(2)∶299-304
[4] 何黎明,郭新程,陶山松,等.CCND1,ORAOV1,ERCC1在舌鳞状细胞癌中的表达和临床意义[J].口腔医学研究,2015,31(6)∶597-600
[5] 朱伟,刘颖芝,李文学,等.玻连蛋白对肝癌细胞株凋亡的抑制效应及可能的机制[J].毒理学杂志,2014,28(3)∶180-184
[6] Ortega Martínez I, Gardeazabal J, Erramuzpe A, et al. Vitronectin and dermcidin serum levels predict the metastatic progression of AJCC I-II early-stage melanoma [J]. Int J Cancer,2016,139(7)∶1598-1607
[7] Jonathan S. Thacker, Derrick H. Yeung,W. Richard Staines, John G. Mielke.Total protein or high-abundance protein:which offers the best loading control for western blotting [J]. Analytical Biochemistry,2016,496∶76-78
[8] 胡梅艳,孙晓红.细胞外基质、基质金属蛋白酶与恶性肿瘤关系的研究进展[J].肿瘤药学,2016,6(1)∶26-30
[9] Kim RH, Coates JM,Bowles TL, et al. Arginine deiminase as a novel therapy for prostate cancer induces autophagy and caspasein dependent apoptosis [J]. Cancer Res,2009,69(2)∶700-708
[10] Xie Y, Mustafa A, Yerzhan A, et al. Nuclear matrix metalloproteinases:functions resemble the evolution from the intracellular to the extracellular compartment [J]. Cell Death Discov,2017,(3)∶17036
[11] 靳勇勇.整合素连接激酶和基质金属蛋白酶-9在涎腺腺样囊性癌中的表达及意义[J].口腔医学研究,2016,32(7)∶764-767

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