Effects of microRNA-210-3p on Proliferation and Differentiation of Human Dental Pulp Cells under Hypoxic Condition.

doi:10.13701/j.cnki.kqyxyj.2019.10.015

Abstract

Abstract: Objective: To evaluate the effects of miR-210-3p on proliferation and differentiation of human dental pulp cells under hypoxic condition. Methods: Human dental pulp cells were isolated, and the expression of miR-210-3p was detected in human dental pulp cells under normoxia and hypoxic condition. After transfection of miR-210-3p inhibitors into human dental pulp cells, the proliferation effect under hypoxia was measured by CCK-8 assay. Alkaline phosphatase staining, real-time PCR and alizarin red staining were adopted to evaluate the regulatory effects of miR-210-3p inhibition on differentiation of human dental pulp cells under hypoxic condition. Results: Human dental pulp cells cultured under hypoxic condition were highly expressed with miR-210-3p. In addition, miR-210-3p promoted proliferation and early differentiation of human dental pulp cells under hypoxia while the late differentiation was inhibited by miR-210-3p in low oxygen. Conclusion: miR-210-3p involves in the regulation of proliferation and differentiation in human dental pulp cells under hypoxic condition.

Key words: microRNA-210-3p, hypoxia, human dental pulp cells, proliferation, differentiation

YUAN Xiaoqin, HUAN Zhongping, WANG Hongtao. Effects of microRNA-210-3p on Proliferation and Differentiation of Human Dental Pulp Cells under Hypoxic Condition.[J]. Journal of Oral Science Research, 2019, 35(10): 970-974.

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