Molecular Mechanism of MicroRNA-9 Influencing Invasion of Salivary Adenoid Cystic Carcinoma Cells

doi:10.13701/j.cnki.kqyxyj.2019.06.013

Abstract

Abstract: Objective: To investigate the effect and regulatory mechanisms of microRNA-9 on invasion of human salivary adenoid cystic carcinoma (SACC) cells. Methods: The expressions of microRNA-9 (miR-9) from ACC-2 cells and ACC-M cells were analyzed using qRT-PCR after transfected with miR-9 lentivirus. Cell invasion was measured by transwell experiment after transfection. Western blot was used to detect the protein expression levels of matrix metalloproteinases family and tumor-related signaling pathways in SACC cell lines after transfection. SPSS 16.0 was used to analyze the data. Results: MiR-9 expression was remarkably down-regulated in the ACC-M cells in contrast to ACC-2 cells (P<0.01). After 72h transfection, the expression of miR-9 was increased in both ACC cells (P<0.01). MiR-9 overexpression triggered to a decreased cell invasion after transfection for ACC-M cells (P<0.01). The expressions of MMP-2 and MMP-9 proteins were significantly decreased after transfection in the ACC-M cells, while AKT protein phosphorylation was significantly decreased. Conclusion: Upregulation of miR-9 can inhibit the invasion of SACC cells. The mechanism might relate to the down-regulation of MMP-2 and MMP-9 mediated by AKT signaling pathway.

Key words: Adenoid cystic carcinoma, MicroRNA, Matrix metalloproteinase, AKT signaling pathway

XU Chuan, LI Ya-nan, QIAO Dan, GU Huan, ZHONG Luo, WU Jian, YU Tao. Molecular Mechanism of MicroRNA-9 Influencing Invasion of Salivary Adenoid Cystic Carcinoma Cells[J]. Journal of Oral Science Research, 2019, 35(6): 563-567.

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