Effect of TRAF6 Knockdown on Osteogenic Differentiation of Human Periodontal Ligament Cell Treated with LPS

doi:10.13701/j.cnki.kqyxyj.2017.11.012

Abstract

Abstract: Objective: To observe the effect of tumor necrosis factor receptor-related factor 6 (TRAF6) knockdown on osteogenic differentiation of human periodontal ligament cell (hPDLC) stimulated by LPS. Methods: The TRAF6 siRNA and control siRNA were transiently transfected into hPDLC. The cells were stimulated with 10μg/ml LPS. The silence efficiency of TRAF6 was detected by RT-PCR and Western blot. The expression of ALP was detected by alkaline phosphatase assay kit. The expression of Runx-2 and type I collagen (Col-I) gene was detected by RT-PCR. Results: The expression of TRAF6 mRNA and protein in TRAF6 siRNA group was significantly decreased (P<0.001). The expression of ALP in LPS-stimulated group was significantly lower than that in non-LPS-stimulated group (P<0.05). The expression of ALP, Runx-2, and Col-I mRNA in TRAF6 siRNA was significantly higher than that in the other three groups (P<0.05). Conclusion: TRAF6 knockdown can alleviate the inhibitory effect of LPS on osteogenesis differentiation of hPDLC, which indicates that TRAF6 may affect the development of periodontitis and may be a potential therapeutic target for periodontitis.

Key words: Tumor necrosis factor, Receptor associated factor 6, RNA interfering, Osteogenic differentiation, Lipopolysaccharides

CLC Number:

R781.4

CHEN Qing-yong, LI Xia, ZHANG Fang, WANG Jin-hua. Effect of TRAF6 Knockdown on Osteogenic Differentiation of Human Periodontal Ligament Cell Treated with LPS[J]. Journal of Oral Science Research, 2017, 33(11): 1181-1184.

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