Preliminary Study on Effect of Yolk Antibody against Prevotella Intermedia on Gingivitis during Pregnancy in Rats.

doi:10.13701/j.cnki.kqyxyj.2019.05.013

Journal of Oral Science Research ›› 2019, Vol. 35 ›› Issue (5): 466-470.DOI: 10.13701/j.cnki.kqyxyj.2019.05.013

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Preliminary Study on Effect of Yolk Antibody against Prevotella Intermedia on Gingivitis during Pregnancy in Rats.

JIANG Peng¹, XU Yan^1*, ZHOU Li-li¹, XU Han-ying¹, XIN Bao-jian¹, YE Xin-ru¹, SHEN Ji-long³, CHENG Ting²

1. College &Hospital of Stomatology, Anhui Medical University, Key Lab. of Oral Diseases Research of Anhui Province, Hefei 230032, China;
2. Anhui Anke Biotechnology (Group) Co.,Ltd, Hefei 230032, China;
3. Anhui Key Laboratory of Zoonoses, Anhui Medical University, Hefei 230032, China.

Received:2018-10-18 Online:2019-05-28 Published:2019-05-21

Abstract

Abstract: Objective: To prepare and purify specific yolk immunoglobulin (IgY) against Prevotella intermedia(P.intermedia, Pi)and to evaluate its therapeutic effect on experimental gingivitis in pregnant rats. Methods: Specific IgY was prepared by two-step ammonium sulfate precipitation method. Twenty-four female SD rats were randomly divided into 4 groups: group A (blank control group), group B (negative group): modeled, group C (positive control group): modeled + tinidazole solution, and group D (experimental group): modeled + specific IgY solution. The therapeutic effect of specific IgY was observed by gingival index (GI), plaque index (PLI), body weight change, RT-qPCR, and histopathological sections. Results: Through the treatment of experimental gestational gingivitis in rats, the GI, PLI (P<0.001) and body weight (P<0.05) were improved, and the composition ratio of P. intermedia was significantly decreased after the treatment of subgingival plaque (P<0.05). Conclusion: Anti-P.intermedia--specific yolk antibody can relief the gingival inflammation in rats during pregnancy.

Key words: Prevotella intermedia, Gingivitis, Real-time quantitative polymerase chain reaction

JIANG Peng, XU Yan, ZHOU Li-li, XU Han-ying, XIN Bao-jian, YE Xin-ru, SHEN Ji-long, CHENG Ting. Preliminary Study on Effect of Yolk Antibody against Prevotella Intermedia on Gingivitis during Pregnancy in Rats.[J]. Journal of Oral Science Research, 2019, 35(5): 466-470.

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Preliminary Study on Effect of Yolk Antibody against Prevotella Intermedia on Gingivitis during Pregnancy in Rats.

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