Abstract: Objective: To observe the effect of TGF-β1 gene modified Bone Marrow Stromal Cells (BMSC) combined with Bio-Oss collagen on jaw defects reconstruction. Methods: 18 beagle dogs were randomly divided into three groups. Control group: implant+BMSC+gelatin+Bio-Oss collagen group A: implant+BMSC transfected with 0.5 μg/L TGF-β1+gelatin+Bio-Oss collagen; group B: implant+BMSC transfected with 10 μg/L TGF-β1+gelatin+Bio-Oss collagen. Histological view was applied to measure the repair effect. Meanwhile, the mRNA and protein expressions of OC, BSP and COL-I were detected through RT-PCR and Western blot, respectively. Results: HE staining showed that bone continuity and maturity in group A were superior to those in group B. RT-PCR results showed that the expressions of mRNA in the experimental group A were significantly higher than those in the control group (P<0.05). However, compared with the control group, the mRNA expression levels in the experimental group B were not statistically significant (P>0.05). In addition, Western blot results showed that expressions of OC, BSP, and COL-I protein in the group A were significantly higher than those of control group and group B (P<0.05), while that of group B was slightly higher than that of control group, but without statistically significant (P>0.05). Conclusion: 0.5 μg/L TGF-β1 gene has a better repair effect on promoting osteogenic differentiation of BMSC.

Key words: TGF-β1 gene, Bone marrow stromal cells, Bio-Oss collagen, Gene and protein expression