Expression and Effect of S100A8 and S100A9 in Chronic Sialadenitis

doi:10.13701/j.cnki.kqyxyj.2022.06.008

Abstract

Abstract: Objective: To investigate the expression and function of S100A8 and S100A9 proteins in chronic submandibular gland sialadenitis. Methods: A rat model of chronic submandibular gland sialadenitis was constructed and human submandibular gland tissues were collected. The histomorphology of chronic sialadenitis and control submandibular glands were observed by HE staining, and the expressions of S100A8 and S100A9 in rat and human chronic submandibular gland tissue were observed by immunohistochemical staining, with aim to explore the mechanism of S100A8 and S100A9 proteins in the progression of chronic sialadenitis. Results: As the ligation time increased, the number of inflammatory cells increased in intralobular interstitium, and the ductal and acinar cells atrophied, accompanied with interstitial fibrosis hyperplasia. The expressions of S100A8 and S100A9 in sialadenitis groups of rats and human were more than those in normal groups. Conclusion: The expression of S100A8 and S100A9 increased with the development of chronic sialadenitis, which could be used as marker proteins for the progression of sialadenitis.

Key words: S100A8, S100A9, submandibular sialadenitis, chronic sialadenitis, calprotectin

WANG Xiaofeng, WEI Lili, CHENG Yong, LI Bo. Expression and Effect of S100A8 and S100A9 in Chronic Sialadenitis[J]. Journal of Oral Science Research, 2022, 38(6): 523-528.

References

[1] Xu JH, Su YX, Cheng MH, et al. Using Tc-99m pertechnetate scintigraphy to predict the outcome of sialoendoscopy in obstructive submaxillaritis [J]. Clin Nucl Med, 2010, 35(2): 77-79.
[2] Korndörfer IP, Brueckner F, Skerra A. The crystal structure of the human (S100A8/S100A9)2 heterotetramer, calprotectin, illustrates how conformational changes of interacting alpha-helices can determine specific association of two EF-hand proteins [J]. J Mol Biol, 2007, 370(5): 887-898.
[3] Silvers R, Stephan JR, Griffin RG, et al. Molecular basis of Ca(Ⅱ)-induced tetramerization and transition-metal sequestration in human calprotectin [J]. J Am Chem Soc, 2021, 143(43): 18073-18090.
[4] Wang S, Song R, Wang Z, et al. S100A8/A9 in inflammation [J]. Front Immunol, 2018, 9: 1298.
[5] Clark HL, Jhingran A, Sun Y, et al. Zinc and manganese chelation by neutrophil S100A8/A9 (calprotectin) limits extracellular aspergillus fumigatus hyphal growth and corneal infection [J]. J Immunol, 2016, 196(1): 336-344.
[6] Ometto F, Friso L, Astorri D, et al. Calprotectin in rheumatic diseases [J]. Exp Biol Med (Maywood), 2017, 242(8): 859-873.
[7] Schrezenmeier EV, Barasch J, Budde K, et al. Biomarkers in acute kidney injury-pathophysiological basis and clinical performance [J]. Acta physiologica (Oxford, England), 2017, 219(3): 554-572.
[8] Sands BE. Biomarkers of inflammation in inflammatory bowel disease [J]. Gastroenterology, 2015, 149(5): 1275-1285.e2.
[9] Dastych M, Gottwaldová J, ermáková Z. Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis? [J]. Clin Chem Lab Med, 2015, 53(4): 599-603.
[10] Sharma R, Deshmukh US, Zheng L, et al. X-linked Foxp3 (Scurfy) mutation dominantly inhibits submandibular gland development and inflammation respectively through adaptive and innate immune mechanisms [J]. J Immunol, 2009, 183(5): 3212-3218.
[11] Kim J, Lee SW, Park K. CXCR4 regulates temporal differentiation via PRC1 complex in organogenesis of epithelial glands [J]. Int J Mol Sci, 2021, 22(2): 619.
[12] Li W, Wei L, Wang F, et al. An experimental chronic obstructive sialadenitis model by partial ligation of the submandibular duct characterised by sialography, histology, and transmission electron microscopy [J]. J Oral Rehabil, 2018, 45(12): 983-989.
[13] Nishii K, Usui M, Yamamoto G, et al. The distribution and expression of S100A8 and S100A9 in gingival epithelium of mice [J]. J Periodontal Res, 2013, 48(2): 235-242.
[14] 孙文华,陈国庆,田卫东.S100A9在糖尿病大鼠牙周组织中的表达及其作用研究[J].华西口腔医学杂志,2016,34(4): 419-424.
[15] Yen T, Harrison CA, Devery JM, et al. Induction of the S100 chemotactic protein, CP-10, in murine microvascular endothelial cells by proinflammatory stimuli [J]. Blood, 1997, 90(12): 4812-4821.
[16] Nukui T, Ehama R, Sakaguchi M, et al. S100A8/A9, a key mediator for positive feedback growth stimulation of normal human keratinocytes [J]. J Cell Biochem, 2008, 104(2): 453-464.
[17] Youssef P, Roth J, Frosch M, et al. Expression of myeloid related proteins (MRP) 8 and 14 and the MRP8/14 heterodimer in rheumatoid arthritis synovial membrane [J]. J Rheumatol, 1999, 26(12): 2523-2528.
[18] Van Lent PL, Grevers L, Blom AB, et al. Myeloid-related proteins S100A8/S100A9 regulate joint inflammation and cartilage destruction during antigen-induced arthritis [J]. Ann Rheum Dis, 2008, 67(12): 1750-1758.
[19] Williams DW, Greenwell-Wild T, Brenchley L, et al. Human oral mucosa cell atlas reveals a stromal-neutrophil axis regulating tissue immunity [J]. Cell, 2021, 184(15): 4090-4104.e15.
[20] Viemann D, Strey A, Janning A, et al. Myeloid-related proteins 8 and 14 induce a specific inflammatory response in human microvascular endothelial cells [J]. Blood, 2005, 105(7): 2955-2962.
[21] Foell D, Wittkowski H, Vogl T, et al. S100 proteins expressed in phagocytes: a novel group of damage-associated molecular pattern molecules [J]. J Leukoc Biol, 2007, 81(1): 28-37.
[22] Ryckman C, Vandal K, Rouleau P, et al. Proinflammatory activities of S100: proteins S100A8, S100A9, and S100A8/A9 induce neutrophil chemotaxis and adhesion [J]. J Immunol, 2003, 170(6): 3233-3242.
[23] Jazzar AA, Shirlaw PJ, Carpenter GH, et al. Salivary S100A8/A9 in Sjögren's syndrome accompanied by lymphoma [J]. J Oral Pathol Med, 2018, 47(9): 900-906.
[24] Rammes A, Roth J, Goebeler M, et al. Myeloid-related protein (MRP) 8 and MRP14, calcium-binding proteins of the S100 family, are secreted by activated monocytes via a novel, tubulin-dependent pathway [J]. J Biol Chem, 1997, 272(14): 9496-9502.
[25] Urban CF, Ermert D, Schmid M, et al. Neutrophil extracellular traps contain calprotectin, a cytosolic protein complex involved in host defense against Candida albicans [J]. PLoS Pathog, 2009, 5(10): e1000639.
[26] Wang J, Vodovotz Y, Fan L, et al. Injury-induced MRP8/MRP14 stimulates IP-10/CXCL10 in monocytes/macrophages [J]. FASEB J, 2015, 29(1): 250-262.
[27] Javkhlan P, Hiroshima Y, Azlina A, et al. Lipopolysaccharide-mediated induction of calprotectin in the submandibular and parotid glands of mice [J]. Inflammation, 2011, 34(6): 668-680.
[28] Li J, Li B, Cheng Y, et al. The synergistic effect of NOD2 and TLR4 on the activation of autophagy in human submandibular gland inflammation [J]. J Oral Pathol Med, 2019, 48(1): 87-95.