Effect of Simvastatin Carried by PLGA/CPC Combining with BMSCs on the Rat Critical Calvarial Defect

Journal of Oral Science Research ›› 2015, Vol. 31 ›› Issue (10): 1032-1035.

Previous Articles Next Articles

Effect of Simvastatin Carried by PLGA/CPC Combining with BMSCs on the Rat Critical Calvarial Defect

YU Xiang-ru^1,2, HAN Xiao-qian³, CHENG Liang¹, GAO Xing¹, ZHANG Xiao-xiao¹, LIU Chang¹

1. Department of Prosthodontics, Stomatology Hospital, Jilin University, Changchun 130021, China;
2. Department of Prosthodontics, Changchun Stomatology Hospita, Changchun 130021, China;
3. Department of Stomatology, Afflited Hospital of Binzhou Medical College, Binzhou 256600, China

Received:2015-04-23 Online:2015-10-28 Published:2016-05-04

Abstract

Abstract: Objective: To investigate the possibilities and restoration effect of simvastatin carried by PLGA/CPC combining with BMSCs for reconstructing tissue-engineering bone in the calvarias of rats with critical sized defects. Methods: A total of 24 male rats were divided into three groups randomly with 8 in each group. Two circular 5 mm diameter defects were prepared on both sides of rat calvarial bone. The 16 calvarial bone defects in each group were randomly divided as simvastatin/PLGA/CPC/BMSCs group (BMSCs signed by Brdu) (n=4), simvastatin/PLGA/CPC group (n=4), PLGA/CPC group (n=4), and blank control group (n=4). After 4, 8, 12 weeks post-operation, all specimens were assessed using general observation, HE, and immunohistochemistry methods to evaluate the bone regeneration. Results: After 4, 8 and 12 weeks post-operation, histological results showed that the bone quality and bone formation quantity at the group with simvastatin/PLGA/CPC/BMSCs were much better than those of other groups. The results of immunohistochemistry showed that the positive expression of osteocalcin at simvastatin/PLGA/CPC/BMSCs group was much higher than that of other three groups. Conclusion: Simvastatin PLGA/CPC scaffold materials combined with BMSCs can induce new bone formation in critical sized calvarial defect, form high quality bone, and shorten the bone healing time when compared with other three groups.

Key words: PLGA Calcium phosphate cement, Critical sized bone defect, Simvastatin, Bone marrow stem cells

CLC Number:

R782.2

YU Xiang-ru, HAN Xiao-qian, CHENG Liang, GAO Xing, ZHANG Xiao-xiao, LIU Chang. Effect of Simvastatin Carried by PLGA/CPC Combining with BMSCs on the Rat Critical Calvarial Defect[J]. Journal of Oral Science Research, 2015, 31(10): 1032-1035.

References

[1] Xu L, Lv K, Zhang W, et al. The healing of critical-size calvarial bone defects in rat with rhPDGF-BB, BMSCss, and β-TCP scaffolds [J]. Journal of Materials Science: Materials in Medicine, 2012, 23(4)∶1073-1084
[2] Senn N. On the healing of aseptic bone cavities by implantation of antiseptic decalcified bone [J]. The American Journal of the Medical Sciences, 1889, 98(3)∶219-247
[3] Dos Santos L A, Carrodeguas R G, Boschi A O, et al. Fiber-enriched double-setting calcium phosphate bone cement [J]. Journal of Biomedical Materials Research Part A, 2003, 65(2)∶244-250
[4] Peter S J, Kim P, Yasko A W, et al. Crosslinking characteristics of an injectable poly (propylene fumarate)/β-tricalcium phosphate paste and mechanical properties of the crosslinked composite for use as a biodegradable bone cement [J]. Cambridge University Press, 1998, 5(3)∶87
[5] Hutmacher D W. Scaffolds in tissue engineering bone and cartilage [J]. Biomaterials, 2000, 21(24)∶2529-2543
[6] Li R H, Wozney J M. Delivering on the promise of bone morphogenetic proteins [J]. Trends in biotechnology, 2001, 19(7)∶255-265
[7] 韩晓谦. 载辛伐他汀 PLGA/CPC 复合骨髓基质干细胞构建组织工程骨的实验研究[D].吉林大学,2013
[8] Ma Li, Liu Da-jun, Li De-tian, et al. Effects of different separate methods and culture conditions on growth proliferation and biological characteristics of rabbit bone marrow mesenchymal stem cells [J]. Journal of Clinical Rehabilitative Tissue Engineering Research,2008,12(1)∶38
[9] Takenaka M, Hirade K, Tanabe K, et al. Simvastatin stimulates VEGF release via p44/p42 MAP kinase in vascular smooth muscle cells [J]. Biochemical and biophysical research communications, 2003, 301(1)∶198-203
[10] Kagami H, Agata H. The potential of somatic stem cells for alveolar bone tissue engineering [J]. Int J Oral-Med Sci, 2010, 9(2)∶1-10
[11] Tai I, Fu Y C, Wang C K, et al. Local delivery of controlled-release simvastatin/PLGA/HAp microspheres enhances bone repair [J]. International journal of nanomedicine, 2013, 8∶3895

Effect of Simvastatin Carried by PLGA/CPC Combining with BMSCs on the Rat Critical Calvarial Defect

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

[1]	XIN Xirui, ZHOU Yanmin. Research Progress of Simvastatin in Promoting Osseointegration and Improving Poor Osteogenic Conditions [J]. Journal of Oral Science Research, 2019, 35(8): 734-737.
[2]	WANG Ke, ZHAO Xu, LI Yi, ZHANG Xue, WANG Yin-jing.. Preparation and Characterization of Hollow Hydroxyapatite Microspheres Loaded with Simvastatin as Pulp Capping Agent. [J]. Journal of Oral Science Research, 2016, 32(7): 707-711.
[3]	ZHANG Wen-li, LI Shu-hui, CHEN Cheng, et al. Study of PRF Combined with Autogenous Bone Marrow Stem Cells on Alveolar Bone Defect. [J]. Journal of Oral Science Research, 2015, 31(4): 336-339.