Journal of Oral Science Research

Study on the Cytoskeleton of Cultured Goat Temporomandibular Joint Disc Cells

KONG Nan-nan, ZHANG Wen-xia, KANG Hong, BAO Guang-jie

2015, 31(10): 953-956.

Abstract ( 225 )

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Objective: To study the morphology and quantify the cytoskeleton (CSK)-actin in primary and passaged goat temporomandibular joint disc (TMJ) cells, and to illustrate the differences of actin protein during passaging using laser scanning confocal microscope (LCSM). Methods: The primary, 1st, 2nd, 3rd and 4th (P0, P1, P2, P3, P4) TMJ disc cells were inoculated into 6-well plates with square coverslips placed in each well. The CSK-actin of each generation of TMJ disc cells was stained by immunofluorescence staining and observed under the LCSM and later quantitatively analyzed by a fluorescence intensity software. Results: Filamentous actin mainly located uniformly at the outer periphery of cell membrane. The CSK-actin became thicker and clearer during cell passaging, and thick fiber bundles formed obviously in P4 generation. The fluorescence intensity also increased during passaging and was significantly higher in P4 than in P1, P2 and P3. Conclusion: The morphology of the CSK-actin changed during the passaging of TMJ disc cells. This finding might provide basis for seed cell choose in TMJ disc engineering.

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Differential Expression of Candida Albicans ALS Gene Family During Biofilm Formation In Vitro

HE Miao, SONG Guang-tai, BIAN Zhuan

2015, 31(10): 957-960.

Abstract ( 186 )

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Objective: To investigate the differential expression of Candida albicans (C. albicans) agglutinin-like sequence (ALS) gene family during biofilm formation on biomaterial surfaces in vitro. Methods: The biofilm model of C. albicans standard strain ATCC 10231 and ATCC 90028 was established in vitro. Expression of the eight members in C. albicans ALS gene family was studied by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) of total RNA from the in vitro biofilm samples on biomaterial surfaces at three distinct developmental phases. Results: ALS1, ALS2, ALS3 and ALS5 transcripts were detected predominantly and consistently over time during biofilm formation. The expression of ALS6, ALS7 and ALS9, especially ALS4, was weak. Transcript amounts of ALS1, ALS2 and ALS3 were up-regulated significantly in intermediate phase and then down-regulated in maturation phase. Strain variability of ALS gene expression was not observed in this study. Conclusion: ALS1, ALS2, ALS3 and ALS5 are predominantly expressed during biofilm formation in vitro. Targeted inhibition of these ALS genes provides potential therapeutic targets to control C. albicans biofilm-associated diseases.

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Fabrication and Characterization of Gallium-containing Hydroxyapatite Coating on Titanium Substrate

HU Xiao-hui, DONG Yi-wen, JIN Yi-fan, MA Jian-feng, LIU Jing-song Wenzhou Medical University, Wenzhou 325027, China

2015, 31(10): 961-964.

Abstract ( 154 )

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Objective: To study the synthesis and characterization of gallium-containing hydroxyapatite coatings. Methods: Gallium-containing hydroxyapatite coatings were prepared by means of sol-gel, which were then deposited on titanium substrates with dip-withdrawal technique. The as-prepared coatings were then calcinated. Surface morphology of the coatings was tested by scanning electron microscope (SEM). The presence of functional groups for coatings was observed by X-ray diffraction spectra (XRD) and Fourier transform infrared spectroscopy spectra (FTIR). The bonding strengths of the samples were performed by pull-out test and the concentrations of Ga3+ and Ca2+ which were released from the coatings were measured by atomic absorption spectrometry (AAS). Results: Compared with HA coatings, the crystallinity of Ga-HA was higher. The concentration of released Ga3+ and Ca2+ decreased after the heat treatment to the coatings. Conclusion: The introduction of Ga increased the crystallinity but little changed the whole structure of HA coatings. The incorporation of Ga decreased the degradation of the coatings. The present study suggested the promising application of Ga-HA coatings.

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Effect of Cytolysin of Enterococcus Faecalis on the Inflammatory Progress of Rat Reinfected Periapical Periodontitis

ZHANG Ming, LU Bing-ling, ZHANG Jin-xiu, HUANG Xiao-jing, LEI Li-shan

2015, 31(10): 965-968.

Abstract ( 173 )

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Objective: To compare the inflammatory progress and the expression of TNF-α in rat-reinfected periapical periodontitis models induced by different genotypes of Enterococcus faecalis (E. faecalis). Methods: After pulp exposure of the bilateral maxillary first molars of rats for 3 weeks, the molars were divided to 4 groups: disinfection group, chronic apical inflammation group, hemolytic E. faecalis reinfected group and nonhemolytic E. faecalis reinfected group. Then the hematoxylin-eosin staining and immumohistochemical staining of TNF-α were conducted. Results: The inflammation degree of the disinfection group was 0. After 1 week in both reinfected groups, the inflammation degree reached to 3, and it lasted to the 3rd week. When E. faecalis was reinfected at the 4th week, the inflammation degree was dropped to 2. It was noticed that the inflammation degree of the nonhemolytic group at the 6th week was reduced to 1 which was the same as chronic apical inflammation group, while the degree of the hemolytic group maintained at level 2 at the same time. The expression of TNF-α was the lowest in the disinfection group. The expression of TNF-α in both reinfected groups was higher than that of the chronic group. At the second week, the expression of TNF-α in both reinfected groups reached the climax, and then decreased. The expression of TNF-α in hemolytic group was higher than that in nonhemolytic group at all time points. Conclusion: Cytolysin (Cyl) may influence the healing progress of E. faecalis reinfected periapical periodontitis and prolong the inflammation process. The expression of TNF-α was related to the inflammation progression of E. faecalis reinfected periapical periodontitis.

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Adhesion and Proliferation of Adipose Derived Stem Cells Sheet on Support Material

LI Fang-fei, LIU Na, YANG Xi, LAN Gui-hua, DENG Man-jing

2015, 31(10): 969-973.

Abstract ( 156 )

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Objective: To construct cells sheet from rat adipose derived mesenchymal stem cells (ADSCs), and investigate its adhesion and proliferation on dentin disc and ceramic bovine bone (CBB). Methods: ADSCs were extracted from adipose tissue of mice at 7 days of age. MTT was used to determine the proliferation capacity of ADSCs and ADSCs sheet on CBB and dentin disc. Multilineage differentiation of ADSCs into osteoblasts, adipocytes, and chondrocytes was also detected. The adhesion ability of ADSCs on CBB and dentin discs was examined by scanning electron microscope (SEM). Results: Purified ADSCs cells had excellent proliferation capacity. They could differentiate into osteoblasts, adipocytes, and chondrocytes. SEM results showed that ADSCs sheet had better adhesion on dentin disc than on CBB paticles. Conclusion: ADSCs sheet has higher proliferation and adhesion ability on dentin disc than CBB particles, which lays the foundation for cells sheet to treat periodontal defects.

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Cell Toxicity and Blood Compatibility of Zr Based Amorphous Alloy for Dentistry

CAO You-ya, SUN Yu, CHENG Xiang, LIU Gui-ying, MA Yue, SUN Ying-bo, WANG Fang 1

2015, 31(10): 974-977.

Abstract ( 180 )

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Objective: To evaluate the cell toxicity and hemo-compatibility of the Zr based amorphous alloy with high strength and modulus of elasticity for dentistry. Methods: Zr based amorphous alloy was performed the hemolysis test by contacting with fresh human blood. MTT method was applied to study the cytotoxicity of this alloy. Results: The level of cell toxicity of Zr based amorphous alloy was 0, which meant no cell toxicity. The Zr based amorphous alloy had low hemolytic rate, long dynamic clotting time, similar anticoagulant capability to pure titanium and Ti6Al4V. The platelet adhesion test implied that Zr-Cu-Al-Ag alloy had seldom pseudopodium phenomenon. Conclusion: Zr-Cu-Al-Ag alloy has adequate cytocompatibilty and blood compatibility, which might meet the basic need of oral implantation.

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Effect of Modified Cerium Oxide Nanoparticles on Mechanical Properties of Denture Resin Base

CHU Ming-hui, GAO Shi-jun, WEI Si-yi, REN Guo-xin, WANG Dong-xia, XIAO Yuan-yuan

2015, 31(10): 978-980.

Abstract ( 220 )

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Objective: To investigate the effect of nano-CeO₂ modified by stearic acid on mechanical properties of denture resin base. Methods: Modified nanoparticles were added in the monomer of denture base materials (PMMA) at the ratios of 0%, 1%, 2%, 3%, 4% and 5% (m:m). Standard specimens were prepared. Impact strength and bending strength were detected in each group. Results: The addition of modified nano-CeO² improved the mechanical properties of resin denture base. The best combination property was obtained at the ratio of 3%. The impact strength, flexural strength and flexural modulus were (4.186 ± 0.149) J/cm², (88.865 ± 2.345) MPa, and (1.539 ± 0.096 ) GPa, respectively (P<0.05). Conclusion: The addition of modified nano-CeO² can improve the mechanical properties of denture resin base.

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Effect of β-SiC Nanowires on Mechanical Properties of POSS Composite Resin

LI Ya-jing, XIE Wei-li, LIU Zi-chuan, SUN Ya-jie, GUO Bin, CHEN Lan-zhu, LIU Yi-zhi

2015, 31(10): 981-983.

Abstract ( 127 )

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Objective: To study effect of different β-SiC nanowires additive on the mechanical properties of POSS composite resin. Methods: β-SiC nanowires were added to POSS composite resin at different mass fraction of 0%, 0.5%, 1.0%, and 1.5%. The mechanical properties of modified resin specimen were detected, including hardness, elasticity modulus, and abrasive resistance. Results: Statistical analysis showed that hardness and elasticity modulus were the highest in the 0.5% group (P<0.05), and the scratch depth in the 0.5% group was the least (P<0.05). Conclusion: Appropriate addition of β-SiC nanowires significantly enhanced the mechanical properties of POSS composite resins.

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Construction of Streptococcus Viridans LuxS Gene Homologous Recombinant Plasmid

LU Xiao, LIU Shao-juan, ZHANG Jin-cai, LIU Qin, LIN Shan, PENG Xiang-ming

2015, 31(10): 984-987.

Abstract ( 143 )

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Objective: To construct a recombination plasmid containing erythromycin resistance gene and the upstream and downstream homologous sequence of LuxS gene of streptococcus viridans (S. viridans) with multidrug resistance. Methods: Erythromycin resistance gene and the upstream and downstream homologous sequence of LuxS were cloned respectively by using plasmid PMG36E and DNA of S. viridans template. Then the genes were ligated into multiple cloning site (MCS) of vector pUC19 and transformed into competent E. coli. Finally, the transformants which were resistant to erythromycin and ampicillin were selected. Results: Erythromycin resistance gene and the upstream and downstream homologous sequence of LuxS were successfully ligated into enzyme digestion site of vector pUC19 accurately. The PCR agarose gel electrophoretic analysis and the sequencing results were correct. Conclusion: The S. viridans recombinant plasmid with LuxS geneknock-out was constructed and could be used for constructing S. viridans LuxS mutans in future.

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Clinical Effect of MTA Apical Barrier Technique Used in the Open Apices Teeth Accompanied with Radicular Cyst

WANG Rong, CAI Yu

2015, 31(10): 988-990.

Abstract ( 218 )

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Objective: To evaluate the clinical effect of MTA apical barrier technique used in the open apices teeth accompanied with radicular cyst. Methods: Twenty-four patients diagnosed with open apices teeth accompanied by radicular cyst were included in this study. All teeth were the upper anterior teeth. Before the surgery all teeth were cleaned and shaped. In the surgery, the open apices were shaped and pluged with 3-5mm MTA after removing the cyst. Until the MTA completely set about one week later, the root filling was performed in the open apices teeth. The patients were followed-up for at least two years. Results: Good result was achieved in all cases. The two-year postoperative X-ray films showed that the apical radiolucent areas of the teeth with pre-existing radicular cyst completely disappeared. No new radiolucency was found around the roots. Conclusion: MTA apical barrier technique is effective in the treatment of open apices teeth accompanied with radicular cyst.

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In Vitro Inhibitive Activity of Metabolites of Bacillus Subtilis Isolated from Oral Cavity

YOU Xiang-lei, WANG Shao-guo, ZENG Sa, CHE Chun-xiao, ZHOU Jian-ye, Wang Jia-jia, LI Zhi-qiang, HE Xiang-yi

2015, 31(10): 991-994.

Abstract ( 190 )

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Objective: To isolate Bacillus subtilis and verify whether its metabolic product could inhibit the major cariogenic bacteria. Methods: The dental plaque was achieved by scratching method, and Bacillus subtilis were then isolated using plate culture method, which were identified through morphology and 16S rDNA gene sequence analysis. The inhibitory activity of the metabolic products against the major cariogenic bacteria was observed by use of the agar well diffusion method. Results: Three strains of bacteria were isolated from the dental plaque, which were identified as Bacillus subtilis through morphology and 16S rDNA gene sequence analysis. The metabolic products showed significant inhibitory activity to Streptococcus mutans, Viscous actinomycetes and Lactobacillus acidophilus, especially Viscous actinomycetes. Conclusion: Bacillus subtilis can be isolated from the dental plaque, and its metabolic products can inhibit the major cariogenic bacteria. Bacillus subtilis is expected to play an important role in prevention and control of caries disease as an inhibitive bacterium.

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Association between Chronic Periodontitis and Vitamin D Receptor Gene Polymorphisms among Uygur Adults in Moyu County of Xinjiang

WU Long, LIN Jing, WANG Dong-lin, ZHAO Jin

2015, 31(10): 995-999.

Abstract ( 167 )

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Objective: To investigate the association between Vitamin D receptor (VDR) gene BsmI and TaqI polymorphisms and chronic periodontitis (CP) among Uygur adults in Moyu County of Xinjiang. Methods: The buccal swabs of 185 subjects, including 95 CP patients and 90 healthy controls, were randomly selected from epidemiological investigation of Uygur adults in Moyu County from March to April 2013. VDR BsmI and TaqI were tested with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to investigate the association between VDR gene polymorphism and CP. Results: There was statistically significant difference in the frequencies of VDR BsmI genotypes and alleles between the CP patients and healthy controls (P=0.016,P=0.003). There was no statistically significant difference in the frequencies of VDR TaqI genotypes and alleles between two groups. Chronic periodontitis and healthy controls only show significant difference in VDR BsmI allele frequencies in male and female. One way logistic analysis showed that bb genotype and allele b might be the risk factors of CP. Conclusion: The susceptibility to chronic periodontitis among Uygur adults in Moyu County of Xinjiang is related to the BsmI polymorphism of VDR gene, the bb genotype and allele b may be the risk factors of CP in Uygur adults.

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Expression and Clinical Significance of IGF-1R and IGFBP-3 in Oral Squamous Cell Carcinoma

JIN Cheng-xue, LI Ming-he, YUE Li-wei, ZHANG Ru-hui

2015, 31(10): 1000-1003.

Abstract ( 146 )

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Objective: To detect the expression of IGF-1R and IGFBP-3 in oral squamous cell carcinoma (OSCC), and explore its clinical and pathological significance. Methods: The expression levels of IGF-1R and IGFBP-3 in 66 cases of OSCC, 20 cases of adjacent tissues of OSCC and 50 cases of normal oral mucosa tissues were detected by immunohistochemistry of S-P staining. The data was statistically analyzed by SPSS19.0. Results: The expression of IGF-1R and IGFBP-3 in OSCC group, adjacent tissue of OSCC and normal group was successively decreased and significantly different (P＜0.05). The expression of IGF-1R and IGFBP-3 was negatively correlated in OSCC tissues (P<0.05). Conclusion: There were significant correlations between the expression of IGF-1R, IGFBP-3 and clinical and pathological features of OSCC.

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Effect of Dental Implant Surgery on Levels of Serum BGP and AKP, Saliva Calcium and Phosphorus in Patients with Postmenopausal Osteoporosis

YANG Lei, ZHANG Li, ZHANG Yong, DAN Jin-xue

2015, 31(10): 1004-1007.

Abstract ( 136 )

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Objective: To investigate the effect of dental implant surgery on levels of serum bone gla protein (BGP), alkline phosphatase (AKP), saliva calcium, and phosphorus in patients with postmenopausal osteoporosis. Methods: Seventy-four patients were grouped according to gender and their bone quality, menopausal status. Their saliva and serum were collected at different time points. Results: The levels of estrone, estradiol, and testosterone in Group A and B were significantly lower than those of Group C (P<0.05). The levels of BGP and AKP in Group A and B were higher those of Control group (P<0.05). Dental implant surgery significantly enhanced the BGP levels. Additionally, the saliva calcium and phosphorus had the same tendency as BGP. Conclusion: Lower estrogen level in postmenopausal patients resulted in osteoporosis, and led to the increase of BGP and AKP in serum, and calcium and phosphorus in saliva compared to the control group. After dental implant surgery, the level of calcium in saliva was positively associated with the level of BGP.

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Effect of Initial Periodontal Therapy on Alveolar Bone in Patients with Chronic Periodontitis: an Evaluation with CBCT

ZHAO Hai-jiao,TAN Li-si, WANG Hong-yan, ZHAO Jian, PAN Chun-ling, PAN Ya-ping

2015, 31(10): 1008-1012.

Abstract ( 148 )

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Objective: To evaluate the difference of alveolar bone height and density of patients with periodontitis before and after initial periodontal therapy by cone beam computed tomography, and to analyze the influences of age and severity degree to provide clinical basis for further therapy design. Methods: Fifty patients with chronic periodontitis were recruited including15 with mild periodontitis, 18 with moderate type and 17 with severe type. The alveolar bone height and bone density were measured with Simplant software and analyzed by t-test to compare the difference between baseline and 6 months after treatment. Results: All patients' alveolar bone height and density improved after 6 months therapy. For the patients younger than 50 years old, alveolar bone density in patients with mild, moderate periodontitis 6 months after treatment were significantly higher than that before treatment (P<0.05). Alveolar bone height in severe periodontitis after treatment also improved, but showed no significant difference. However, the efficacy of therapy decreased as age increased, as there was no significant difference between before and after treatment. As to different tooth sites, mesial and distal sites of premolar and molar improved much more than other sites, as demonstrated by changes of both alveolar bone height and density (P<0.05). Conclusion: Initial periodontal therapy can effectively improve the alveolar bone height and density of patients with chronic periodontitis. CBCT and Simplant software can help to evaluate changes of alveolar bone throughout the treatment.

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Three Dimensional Finite Element Analysis of Defected Mandibular Premolar Restored with Post and Core Crown

SONG Liang, XU Bin, CHEN Hui-juan, Liu Liu-hui, HU Kai-ming

2015, 31(10): 1013-1016.

Abstract ( 160 )

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Objective: To investigate the effect of different degrees of dentin defect on the stress distribution of post and core restored mandibular premolar by three dimension finite element analysis. Methods: Spiral CT based three-dimensional finite element models of post and core restored mandibular premolar were established. The ferrule heights of the defective area were respectively 0mm, 1mm, 2mm. The defect sites and extent were respectively the whole crown defect, 1/3 of the mesial and distal proximal surface, 1/2 of distal proximal surface, and 1/3 of distal proximal surface. The stress distribution of the defect models restored by fiber post and resin core was analyzed. Results: The maximum shear stress occurred in the whole crown defect models and the models without ferrule. The maximum principal stress appeared in the mesiodistal dentin defect models. Conclusion: Fiber post and resin core restoration should be avoided where the dentin ferrule is not left. When the buccal-lingual dentin width is insufficient, the dentin ferrule height should be increased to secure the fiber post and resin core restoration.

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Application of ⁹⁹Tc^m-MIBI SPECT-CT for Differential Diagnosis of Benign and Malignant Parotid Tumors

LIU Hong-wei, LI Ning-yi

2015, 31(10): 1017-1019.

Abstract ( 123 )

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Objective: To evaluate the clinical application of ⁹⁹Tc^m-methoxyisobutylis-onitrile (⁹⁹Tc^m-MIBI) SPECT-CT for differential diagnosis of benign and malignant parotid tumors before treatment. Methods: SPECT-CT fusion images of parotid regions were obtained from 30 patients with unilateral parotid gland tumor before surgery. ^99mTc-MIBI was used as the imaging agent. The results were compared with their pathological diagnoses and clinical examination. The chi-square test and t-test were used for statistical analysis. Results: The sensitivity, specificity and accuracy of ⁹⁹Tc^m-MIBI SPECT-CT fusion imaging in determining the malignant parotid tumors were 84.61%, 94.12% and 90.00%, respectively. Sixteen of 17 patients with benign tumors showed negative images (94.11%, 16/17), while 1 of 17 patients showed positive images (5.89%, 1/17). In addition, 12 of 13 patients with malignant tumors showed positive images (92.31%, 12/13), while 1 of 13 patients showed negative image (7.69%, 1/13). There were significant differences between two groups by chi-square test. The uptake ratios of benign and malignant parotid tumors were 1.42±0.6 and 2.32±0.58 (P＜0.05), respectively. Conclusion: ⁹⁹Tc^m-MIBI SPECT-CT might be a useful method for differential diagnosis of benign and malignant parotid tumors before treatment.

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Study of Fluid Shear Stress on Osteogenic Differentiation of Human Adipose-derived Stromal Cells in Vitro

CUI Jun, JIA Li, LIU Xue-hen, CUI Guang-hui

2015, 31(10): 1020-1023.

Abstract ( 160 )

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Objective: To investigate the influence of fluid shear stress (FSS) on osteogenic differentiation of human adipose-derived stromal cells (hADSCs) in vitro. Methods: hADSCs were cultured and proliferated in vitro, and subjected to a FSS of 3 dyne/cm2 for 30min every day. Then the hADSCs were observed under inverted microscope. The growth curve was evaluated by cell differentiation ability which was measured by MTT. Moreover, the osteoblastic differentiation ability was also tested by ALP activity and mineralization activity. Results: After treated by FSS, hADSCs were characterized by large size with a relatively large nucleus and plenty cytoplasm. The orientation of hADSCs growth was consistent with the direction of FSS and the growth rate was enhanced by FSS. What's more, ALP activity and mineralization activity of hADSCs were both increased significantly after FSS stimulation. Conclusion: hADSCs exhibited the osteoblastic differentiation ability after treated with FSS, which could be used as the seed cells for bone tissue engineering.

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Quantitative Analysis of Triangular Vermilion Flap in Unilateral Cleft Lip Repair

WANG Shan, YANG Xue-cai, LIU Mei-xia, SUN Jian, CHEN Li-qiang, DU De-jiang

2015, 31(10): 1024-1028.

Abstract ( 132 )

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Objective: To analyze the preoperative data for the design of vermilion triangular flap, and evaluate the reconstruction effect by quantitative analysis of postoperative data in vermilion reconstruction in unilateral cleft lip. Methods: The measured data before and after operation in 26 cases of unilateral cleft were compared with the healthy side in the Department of Oral and Maxillofacial Surgery of the Affiliated Hospital of Qingdao University. The preoperative measured data included bilateral lip length, dry vermilion length, flap and bottom length of both cleft and non-cleft sides. The relationship of preoperative data were analyzed by medical statistics methods and used for designing the vermilion triangular flap. The postoperative measured data included lip length, dry vermilion and lip height of both cleft and non-cleft side. The correlation of the above data was analyzed by medical statistics method. The results of the designed vermilion triangular were evaluated from the subjective and objective aspects, including the shape and symmetry of lip vermilion. Results: There was a significant correlation between the degree of cleft lip fissure and the design of bottom length of vermilion triangular. The preoperative height difference was positively correlated with the bottom length of vermilion triangular (P＜0.05). The average scores for subjective evaluation items were more than four in all 26 cases. Objective evaluation of postoperative height of dry vermilion and lip in both cleft and non-cleft side showed no differences. Conclusion: The triangular vermilion flap designed in this paper can achieve satisfied upper lip vermilion in the unilateral cleft lip repair.

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Application of PDCA in the Quality Control of Root Canal Obturation

TONG Fang-li, ZENG Xiong-qun, LI Mei, YUAN Pei-yan, WANG Ying-ying, CHEN Lei

2015, 31(10): 1029-1031.

Abstract ( 137 )

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Objective: To evaluate the effect of PDCA on the quality control of root canal obturation. Methods: The root canal proper-filling rate before and after the application of PDCA was compared. Results: The total root canal proper-filling rate reached the PDCA target. The root canal proper-filling rate of molar improved from 85.84% to 90.45%. There was significant difference in the molar root canal proper-filling rate before and after the PDCA cycle. Conclusion: The application of PDCA facilitates the improvement of molar's root canal obturation and excerts a positive effect on the quality control of root canal obturation.

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Effect of Simvastatin Carried by PLGA/CPC Combining with BMSCs on the Rat Critical Calvarial Defect

YU Xiang-ru, HAN Xiao-qian, CHENG Liang, GAO Xing, ZHANG Xiao-xiao, LIU Chang

2015, 31(10): 1032-1035.

Abstract ( 145 )

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Objective: To investigate the possibilities and restoration effect of simvastatin carried by PLGA/CPC combining with BMSCs for reconstructing tissue-engineering bone in the calvarias of rats with critical sized defects. Methods: A total of 24 male rats were divided into three groups randomly with 8 in each group. Two circular 5 mm diameter defects were prepared on both sides of rat calvarial bone. The 16 calvarial bone defects in each group were randomly divided as simvastatin/PLGA/CPC/BMSCs group (BMSCs signed by Brdu) (n=4), simvastatin/PLGA/CPC group (n=4), PLGA/CPC group (n=4), and blank control group (n=4). After 4, 8, 12 weeks post-operation, all specimens were assessed using general observation, HE, and immunohistochemistry methods to evaluate the bone regeneration. Results: After 4, 8 and 12 weeks post-operation, histological results showed that the bone quality and bone formation quantity at the group with simvastatin/PLGA/CPC/BMSCs were much better than those of other groups. The results of immunohistochemistry showed that the positive expression of osteocalcin at simvastatin/PLGA/CPC/BMSCs group was much higher than that of other three groups. Conclusion: Simvastatin PLGA/CPC scaffold materials combined with BMSCs can induce new bone formation in critical sized calvarial defect, form high quality bone, and shorten the bone healing time when compared with other three groups.

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Comparison of Cone Beam CT and Periapical Film in Diagnosis of Tooth Root Fracture

WANG Xiao-le, LIAO Jian-lin, DING Shui-qing, CHEN Jian-rong, LI Dong-ping

2015, 31(10): 1037-1039.

Abstract ( 181 )

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Objective: To compare the diagnostic value of cone beam CT (CBCT) and periapical film in tooth root fracture. Methods: Thirty-seven teeth from 35 patients were included in this study whose roots were fracture confirmed after extraction or surgical exploration. All teeth were examined by both CBCT and periapical film. Radiological images were analyzed and compared. Results: Of the 37 fractured teeth, CBCT detected 35 teeth and periapical film detected 16 teeth. Conclusion: The accuracy of CBCT in detection of tooth root fracture was significantly higher than that of periapical film. The CBCT clearly showed the location and type of root fracture. The CBCT held important clinical value in the treatment planning of tooth root fracture.

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Expression of HMGB1 in Tongue Squamous Cell Carcinoma

LI Jing, ZOU Tin-qian, WANG Wei, LI Chen-feng, ZENG Tao

2015, 31(10): 1040-1042.

Abstract ( 142 )

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Objective: To investigate the expression and biological significance of HMGB1 proteinin tissue specimens of tongue squamous cell carcinoma (TSCC), and study the correlation between HMGB1 and clinical features of the patients. Methods: The expression of HMGB1 protein was evaluated by immunohistochemical staining in 51 cases of TSCC specimens and further correlated with clinical pathologic parameters of the patients using statistical methods. Results: The positive rates of HMGB1 in TSCC tissues were 92.2%. The expression of HMGB1 was not significantly correlated with tumor histological differentiation, however it was closely correlated with lymph node metastasis (P<0.05) in TSCC. There were 81.2% high expression of HMGB1 in lymph node metastasis, while only 32.6 % in no metastasis cases. The Kaplan-Meier survival analysis showed that patients with strong expression of HMGB1 had poorer overall survival rate compared with that in patients with relatively low HMGB1 expression (P<0.05) . Conclusion: HMGB1 over-expression was closely associated with metastasis and poorer prognosis inpatients with TSCC.

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Research Progress of Freeze-drying and Combined Application of PRP and PRF

LIU Li-sha, ZHANG Bin, LI Yin

2015, 31(10): 1047-1049.

Abstract ( 187 )

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Platelet concentrates for surgical use are innovative tools of regenerative medicine. Platelet-rich plasma (PRP) is the first generation of platelet concentrate that has been used widely to accelerate soft-tissue and hard-tissue healing. Platelet-rich fibrin (PRF), a new generation of platelet concentrates, is first described by Choukroun et al.It has been shown to have several advantages over traditional PRP. Freeze-drying is the conventional method to save biological products andis also an effective means to control scaffold pore size and preserve its composition. This article reviewed PRP, PRF, freeze-drying and the combined application in organization development and regeneration.

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Current Concepts in Mechanical Stimuli on Bone Remodeling

WANG Zhu-yu, ZHANG Xiao-lei

2015, 31(10): 1050-1052.

Abstract ( 198 )

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A wide range of researches have demonstrated that mechanical stimuli play an important role in bone formation and remodeling. Osteoclasts, osteoblasts and osteocytes are able to perceive external mechanical stimuli through various sensors such as integrins, focal adhesion and cadherin, and then activate downstream signaling pathways including MAPK pathways, β-catenin pathways, and GTPase cascades, to affect the development and metabolism of bone tissue. This review intends to expound recent progress in this field, of signaling pathways related to mechanical stimuli.

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Research Progress of Icariin in Oral Molecular Biology and Cell Physiology

GAO Jie, ZHANG Ji-xia

2015, 31(10): 1053-1056.

Abstract ( 141 )

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In recent years, the research of icariin in oral medicine is growing. To further enhance the research of icariin in prevention and treatment of oral disease, after reviewed the domestic and international studies, this article systematically illustrated the molecular biology and cell physiological effects of icariin on tooth development, periodontal tissues, and alveolar bone tissue.

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