Construction of Streptococcus Viridans LuxS Gene Homologous Recombinant  Plasmid

Abstract

Abstract: Objective: To construct a recombination plasmid containing erythromycin resistance gene and the upstream and downstream homologous sequence of LuxS gene of streptococcus viridans (S. viridans) with multidrug resistance. Methods: Erythromycin resistance gene and the upstream and downstream homologous sequence of LuxS were cloned respectively by using plasmid PMG36E and DNA of S. viridans template. Then the genes were ligated into multiple cloning site (MCS) of vector pUC19 and transformed into competent E. coli. Finally, the transformants which were resistant to erythromycin and ampicillin were selected. Results: Erythromycin resistance gene and the upstream and downstream homologous sequence of LuxS were successfully ligated into enzyme digestion site of vector pUC19 accurately. The PCR agarose gel electrophoretic analysis and the sequencing results were correct. Conclusion: The S. viridans recombinant plasmid with LuxS geneknock-out was constructed and could be used for constructing S. viridans LuxS mutans in future.

Key words: Streptococcus viridans, LuxS gene, Vector pUC19, Plasmid, PMG36E

CLC Number:

R780.1

LU Xiao, LIU Shao-juan, ZHANG Jin-cai, LIU Qin, LIN Shan, PENG Xiang-ming. Construction of Streptococcus Viridans LuxS Gene Homologous Recombinant Plasmid[J]. Journal of Oral Science Research, 2015, 31(10): 984-987.

References

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Construction of Streptococcus Viridans LuxS Gene Homologous Recombinant Plasmid

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