Differential Expression of Candida Albicans ALS Gene Family During Biofilm Formation In Vitro

Abstract

Abstract: Objective: To investigate the differential expression of Candida albicans (C. albicans) agglutinin-like sequence (ALS) gene family during biofilm formation on biomaterial surfaces in vitro. Methods: The biofilm model of C. albicans standard strain ATCC 10231 and ATCC 90028 was established in vitro. Expression of the eight members in C. albicans ALS gene family was studied by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) of total RNA from the in vitro biofilm samples on biomaterial surfaces at three distinct developmental phases. Results: ALS1, ALS2, ALS3 and ALS5 transcripts were detected predominantly and consistently over time during biofilm formation. The expression of ALS6, ALS7 and ALS9, especially ALS4, was weak. Transcript amounts of ALS1, ALS2 and ALS3 were up-regulated significantly in intermediate phase and then down-regulated in maturation phase. Strain variability of ALS gene expression was not observed in this study. Conclusion: ALS1, ALS2, ALS3 and ALS5 are predominantly expressed during biofilm formation in vitro. Targeted inhibition of these ALS genes provides potential therapeutic targets to control C. albicans biofilm-associated diseases.

Key words: Candida albicans, Agglutinin-like sequence, Biofilm

CLC Number:

R780.1

HE Miao, SONG Guang-tai, BIAN Zhuan. Differential Expression of Candida Albicans ALS Gene Family During Biofilm Formation In Vitro[J]. Journal of Oral Science Research, 2015, 31(10): 957-960.

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