Study on the Cytoskeleton of Cultured Goat Temporomandibular Joint Disc Cells

Abstract

Abstract: Objective: To study the morphology and quantify the cytoskeleton (CSK)-actin in primary and passaged goat temporomandibular joint disc (TMJ) cells, and to illustrate the differences of actin protein during passaging using laser scanning confocal microscope (LCSM). Methods: The primary, 1st, 2nd, 3rd and 4th (P0, P1, P2, P3, P4) TMJ disc cells were inoculated into 6-well plates with square coverslips placed in each well. The CSK-actin of each generation of TMJ disc cells was stained by immunofluorescence staining and observed under the LCSM and later quantitatively analyzed by a fluorescence intensity software. Results: Filamentous actin mainly located uniformly at the outer periphery of cell membrane. The CSK-actin became thicker and clearer during cell passaging, and thick fiber bundles formed obviously in P4 generation. The fluorescence intensity also increased during passaging and was significantly higher in P4 than in P1, P2 and P3. Conclusion: The morphology of the CSK-actin changed during the passaging of TMJ disc cells. This finding might provide basis for seed cell choose in TMJ disc engineering.

Key words: TMJ disc cell, Passage, Cytoskeleton, Laser scanning confocal microscope

CLC Number:

R782.6

KONG Nan-nan, ZHANG Wen-xia, KANG Hong, BAO Guang-jie. Study on the Cytoskeleton of Cultured Goat Temporomandibular Joint Disc Cells[J]. Journal of Oral Science Research, 2015, 31(10): 953-956.

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