Effects of Cytochalasin B on Function and Actin Cytoskeleton of Goat Temporomandibular Joint Disc Cells.

doi:10.13701/j.cnki.kqyxyj.2016.08.001

Abstract

Abstract: Objective: To investigate the relationship between cytoskeletal integrity and the functional changes of temporomandibular join disc cells by cytochalasin B(CB). Methods: Taking cell proliferation and morphology as evaluating parameters, different concentrations of CB were added to TMJ disc cells to get an optimal working concentration. Cells exposing to the optimal concentration of CB, apoptosis, adhesion and actin were observed. Results: CB of 2 μmol/L obviously inhibited the proliferative activity of TMJ disc cells. However, as the culturing went on, the inhibition rate significantly reduced. No effect was observed with regard to cell apoptosis. The TMJ disc cell adhesion rate reduced. Compare with the control group, cells gradually lost ecptomas in the treatment group, becoming smoother; actin filaments obviously fractured and fluorescence intensity decreased. Moreover, actin mRNA expression reduced. Conclusion: CB may inhibit TMJ disc cell morphology and function by destroying the structure of actin.

Key words: Temporomandibular join disc, Cytochalasin B, Actin, Cytoskeleton

CLC Number:

R782.6

ZHANG Wen-xia, KANG Hong, LV Wei, LI Yan-mei, ZHONG Ni, BAO Guang-jie. Effects of Cytochalasin B on Function and Actin Cytoskeleton of Goat Temporomandibular Joint Disc Cells.[J]. Journal of Oral Science Research, 2016, 32(8): 775-779.

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