Effect of Mechanical Injury on the Expression of Notch Signaling and Apoptosis in Odontoblasts of Mouse Incisor.

doi:10.13701/j.cnki.kqyxyj.2016.08.002

Abstract

Abstract: Objective: To investigate Notch expression and apoptosis in odontoblasts of mouse incisor after mechanical injury. Methods: Dental mechanical injury model was established through the cavity preparation on the mandibular incisor of mice. The expressions of Notch1 and Notch2 in the odontoblasts were observed by immunohistochemical staining, and apoptosis of the odontoblasts was observed by TUNEL staining. Results: The expression of Notch in odontoblasts were activated by mechanical injury and showed a dynamic change. One day after injury, the expressions of Notch1 and Notch2 were positive; 3 days after injury, the expressions of Notch1 and Notch2 were significantly increased and were intensively positive; 5 days after injury, Notch1 and Notch2 were still positive; 7 days after injury, the expressions of Notch1 and Notch2 became weak. In control, expressions of Notch1 and Notch2 were very weak. Mechanical injury leaded to massive apoptosis in dental pulp. Apoptosis staining in the odontoblasts was obvious 1 day after injury, and the apoptotic cells decreased gradually from 3-7 days after injury. Few apoptotic odontoblasts were observed in the control group. Concomitant pattern of the cell apoptosis and Notch expression in the odontoblasts were exhibited after mechanical injury of tooth. Conclusion: Dynamic activation of Notch signaling might be connected with apoptosis regulation in odontoblasts after mechanical injury.

Key words: Odontoblast, Notch signaling, Apoptosis, Mechanical injury

CLC Number:

R780.1

YIN Qiu-rong, WANG Sheng-chao, LUO Ying, TONG Juan, HU Yi. Effect of Mechanical Injury on the Expression of Notch Signaling and Apoptosis in Odontoblasts of Mouse Incisor.[J]. Journal of Oral Science Research, 2016, 32(8): 780-784.

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