Abstract: Objective: To construct cells sheet from rat adipose derived mesenchymal stem cells (ADSCs), and investigate its adhesion and proliferation on dentin disc and ceramic bovine bone (CBB). Methods: ADSCs were extracted from adipose tissue of mice at 7 days of age. MTT was used to determine the proliferation capacity of ADSCs and ADSCs sheet on CBB and dentin disc. Multilineage differentiation of ADSCs into osteoblasts, adipocytes, and chondrocytes was also detected. The adhesion ability of ADSCs on CBB and dentin discs was examined by scanning electron microscope (SEM). Results: Purified ADSCs cells had excellent proliferation capacity. They could differentiate into osteoblasts, adipocytes, and chondrocytes. SEM results showed that ADSCs sheet had better adhesion on dentin disc than on CBB paticles. Conclusion: ADSCs sheet has higher proliferation and adhesion ability on dentin disc than CBB particles, which lays the foundation for cells sheet to treat periodontal defects.

Key words: Adipose derived stem cells, Cells sheet, Periodontal tissue regeneration, Dentin disc