Study of Fluid Shear Stress on Osteogenic Differentiation of Human Adipose-derived Stromal Cells in Vitro

Abstract

Abstract: Objective: To investigate the influence of fluid shear stress (FSS) on osteogenic differentiation of human adipose-derived stromal cells (hADSCs) in vitro. Methods: hADSCs were cultured and proliferated in vitro, and subjected to a FSS of 3 dyne/cm2 for 30min every day. Then the hADSCs were observed under inverted microscope. The growth curve was evaluated by cell differentiation ability which was measured by MTT. Moreover, the osteoblastic differentiation ability was also tested by ALP activity and mineralization activity. Results: After treated by FSS, hADSCs were characterized by large size with a relatively large nucleus and plenty cytoplasm. The orientation of hADSCs growth was consistent with the direction of FSS and the growth rate was enhanced by FSS. What's more, ALP activity and mineralization activity of hADSCs were both increased significantly after FSS stimulation. Conclusion: hADSCs exhibited the osteoblastic differentiation ability after treated with FSS, which could be used as the seed cells for bone tissue engineering.

Key words: Fluid shear stress, Adipose-derived stromal cell, Osteogenic differentiation, Bone tissue engineering

CLC Number:

R782.2

CUI Jun, JIA Li, LIU Xue-hen, CUI Guang-hui. Study of Fluid Shear Stress on Osteogenic Differentiation of Human Adipose-derived Stromal Cells in Vitro[J]. Journal of Oral Science Research, 2015, 31(10): 1020-1023.

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