In Vitro Inhibitive Activity of Metabolites of Bacillus Subtilis Isolated from Oral Cavity

Abstract

Abstract: Objective: To isolate Bacillus subtilis and verify whether its metabolic product could inhibit the major cariogenic bacteria. Methods: The dental plaque was achieved by scratching method, and Bacillus subtilis were then isolated using plate culture method, which were identified through morphology and 16S rDNA gene sequence analysis. The inhibitory activity of the metabolic products against the major cariogenic bacteria was observed by use of the agar well diffusion method. Results: Three strains of bacteria were isolated from the dental plaque, which were identified as Bacillus subtilis through morphology and 16S rDNA gene sequence analysis. The metabolic products showed significant inhibitory activity to Streptococcus mutans, Viscous actinomycetes and Lactobacillus acidophilus, especially Viscous actinomycetes. Conclusion: Bacillus subtilis can be isolated from the dental plaque, and its metabolic products can inhibit the major cariogenic bacteria. Bacillus subtilis is expected to play an important role in prevention and control of caries disease as an inhibitive bacterium.

Key words: Bacillus subtilis, Metabolic product, Inhibitive activity, Cariogenic bacteria

CLC Number:

R780.1

YOU Xiang-lei, WANG Shao-guo, ZENG Sa, CHE Chun-xiao, ZHOU Jian-ye, Wang Jia-jia, LI Zhi-qiang, HE Xiang-yi. In Vitro Inhibitive Activity of Metabolites of Bacillus Subtilis Isolated from Oral Cavity[J]. Journal of Oral Science Research, 2015, 31(10): 991-994.

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