Construction of Doxycycline-inducible Adenoviral Vector-mediated Expression of Basic Fibroblast Growth Factor in Vitro

Journal of Oral Science Research ›› 2015, Vol. 31 ›› Issue (9): 851-855.

Construction of Doxycycline-inducible Adenoviral Vector-mediated Expression of Basic Fibroblast Growth Factor in Vitro

SONG Ke, CHEN Mei-ling, SHI Qi, QING Ying, CAO Ying-guang

Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Received:2015-07-27 Online:2015-09-28 Published:2016-05-04

Abstract

Abstract: Objective: To establish the dox-inducible adenoviral vector and to evaluate its ability to deliver bFGF to cells in bone tissue engineering. Methods: The adenoviral vector pAAV-S3-bFGF was constructed. Adenovirus was packaged in HEK293 cells and then infected the mouse osteoblastic cells (MC3T3-E1). The efficiency of infection was evaluated by real-time PCR and Western blot. Results: The recombinant adenovirus rAAV2-tet-off-bFGF was regulated by Dox. MC3T3-E1 cells could be efficiently infected. Additionally, the expression of osteogenesis-related genes was significantly increased in vitro. Conclusion: The rAAV2-tet-off-bFGF can deliver exogenous genes efficiently into MC3T3-E1 cells, and has the potential to be used in bone tissue engineering.

Key words: Adeno-associated virus, Gene delivery, Basic fibroblast growth factor, Tetracycline regulated system

CLC Number:

R782.1

SONG Ke, CHEN Mei-ling, SHI Qi, QING Ying, CAO Ying-guang. Construction of Doxycycline-inducible Adenoviral Vector-mediated Expression of Basic Fibroblast Growth Factor in Vitro[J]. Journal of Oral Science Research, 2015, 31(9): 851-855.

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