Temporal and Spatial Expression of Pik3cb at Different Developmental Stages of Mouse Mandibular First Molar.

doi:10.13701/j.cnki.kqyxyj.2017.03.005

Abstract

Abstract: Objective: To detect the expression pattern and variation of Pik3cb at different developmental stages of mouse mandibular first molar. Methods: The probe for in situ hybridization was prepared. Mouse embryos at different times were obtained to get cryostat serial sections of tooth germ for ISH. Results: In bud period, Pik3cb obviously expressed in dental lamina epithelium. In bonnet period, the Pik3cb began to express in the enamel epithelium and dental papilla. In bell stage, the expression of Pik3cb was especially within the enamel epithelium and subsequently in the new formation of hard tissues surrounding the columnar cells into glaze. Conclusion: Pik3cb expresses in dental epithelial cells and ameloblasts and it is likely to associate with ameloblastoma through PI3K/PTEN/AKT/mTOR signaling pathway.

Key words: PI3K/PTEN/AKT/mTOR, Molar tooth germ , Tooth development, In situ hybridization(ISH)Ameloblastoma

CLC Number:

Q132

ZHANG Yang, CHAI Ji-hua, REN Ming-xin, WANG Ge. Temporal and Spatial Expression of Pik3cb at Different Developmental Stages of Mouse Mandibular First Molar.[J]. Journal of Oral Science Research, 2017, 33(3): 254-257.

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