Temporal and Spatial Expression of Ddit3 during the First Mandibular Mouse Molar Tooth Germs Development.

doi:10.13701/j.cnki.kqyxyj.2016.04.004

Abstract

Abstract: Objective: To investigate the temporal and spatial expression pattern of Ddit3 during the first molar development and tentatively uncover the functional role of Ddit3 during tooth development. Methods: Time pregnancy ICR mouse embryos was obtained and serially sectioned for immunofluorescence and immunocytochemistry staining. Results: During the early development, Ddit3 was mainly expressed in the cytoplasm of mouse first molar tooth germ. From the early bell stage, Ddit3 was not only expressed in the cytoplasm, but also weakly expressed in the nucleus. Expression pattern was as follows: in the laminae stage, there was no expression of Ddit3 neither in oral epithelium and mesenchymal cells; in the bud stage, the expression of Ddit3 was located in the cytoplasm of dental epithelium cells as well as in the cap stage; in the early bell stage, Ddit3 had an expression in the cytoplasm of ameloblasts, pre-odontoblasts and dental papilla cells. Ddit3 was also expressed in the nucleus of those cells. In the late bell stage, Ddit3 was expressed in the cytoplasm of high columnar ameloblasts and odontoblasts nearby the newly formed hard tissues. Furthermore, Ddit3 was also expressed in the nucleus of the most ameloblasts, odontoblasts and dental papilla cells. Conclusion: Ddit3 may play a vital role in the differentiation of ameloblasts, odontoblasts and biomineralization process.

CLC Number:

Q132

YU Miao, WU Yan-ru, HAN Yu-ting, TENG Fang-jun, HUANG Yi-qing, WANG Jia-wei.. Temporal and Spatial Expression of Ddit3 during the First Mandibular Mouse Molar Tooth Germs Development.[J]. Journal of Oral Science Research, 2016, 32(4): 331-334.

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